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Neuropeptide Y receptor Y8b (
npy8br ) regulates feeding and digestion in Japanese medaka (Oryzias latipes ) larvae: evidence from gene knockoutNeuropeptide Y receptor Y8b (
npy8br ) regulates feeding and digestion in Japanese medaka (Oryzias latipes ) larvae: evidence from gene knockout- 神经肽受体Y8b（
npy8br ）调节日本青鳉（Oryzias latipes ）仔鱼的摄食和消化： 来自基因敲除的证据 - Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Vol. 25, Issue 7, Pages: 605-616(2024)
- Affiliations：
1.College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China
2.Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China
- Funds：
DOI：10.1631/jzus.B2300312
CLC：- CSTR：
Published： 15 July 2024 ，
Published Online： 05 June 2024 ，
Received： 09 May 2023 ，
Revised： 05 September 2023 ，
- Accepted：
贾晓丹,陆可,梁旭方.神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化： 来自基因敲除的证据[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(07):605-616.
Xiaodan JIA, Ke LU, Xufang LIANG. Neuropeptide Y receptor Y8b (
npy8br ) regulates feeding and digestion in Japanese medaka (Oryzias latipes ) larvae: evidence from gene knockout. [J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 25(7):605-616(2024)贾晓丹,陆可,梁旭方.神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化： 来自基因敲除的证据[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(07):605-616. DOI： 10.1631/jzus.B2300312.
Xiaodan JIA, Ke LU, Xufang LIANG. Neuropeptide Y receptor Y8b (
npy8br ) regulates feeding and digestion in Japanese medaka (Oryzias latipes ) larvae: evidence from gene knockout. [J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 25(7):605-616(2024) DOI： 10.1631/jzus.B2300312.
摘要
神经肽Y受体Y8（NPY8R）是一种鱼类特异性受体，具有NPY8AR和NPY8BR两种亚型。生理过程中表达水平的变化以及心室注射后体内调节过程表明，NPY8BR在摄食调节中发挥着重要作用；目前只在少数鱼类中发现有这种作用。为了更好地了解NPY8BR的生理功能，特别是消化方面，我们利用CRISPR/Cas9技术构建了
npy8br
-/-
日本青鳉（
Oryzias latipes
）。实验结果表明，
npy8br
基因缺失会影响青鳉仔鱼的摄食和消化能力，并最终影响其生长。具体来说，缺失
npy8br
会导致鳉鱼仔鱼摄食量减少和食欲相关基因（
npy
、
agrp
）表达水平降低。饲喂10 d（饲喂第10天）的
npy8br
-/-
青鳉仔鱼在摄食8 h后消化道内仍有未完全消化的卤虫（
Artemia nauplii
），消化相关基因（
amy
、
lpl
、
ctra
和
ctrb
）mRNA表达量显著降低，且淀粉酶、胰蛋白酶和脂肪酶活性也显著降低。
npy8br
的缺失抑制了青鳉仔鱼的生长，显著降低了生长相关基因（
gh
和
igf1
）的表达。肠道组织切片显示，
npy8br
-/-
青鳉仔鱼肠道受损，肠壁变薄，肠绒毛变短。本研究首次成功构建了鱼类的
npy8br
基因敲除模型，并证明
npy8br
在消化过程发挥着重要作用。
Abstract
Neuropeptide Y receptor Y8 (NPY8R) is a fish-specific receptor with two subtypes
NPY8AR and NPY8BR. Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation; this has been found in only a few fish
at present. In order to better understand the physiological function of
npy8br
especially in digestion
we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology to generate
npy8br
-/-
Japanese medaka (
Oryzias latipes
). We found that the deletion of
npy8br
in medaka larvae affected their feeding and digestion ability
ultimately affecting their growth. Specifically
npy8br
deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes (
npy
and
agrp
).
npy8br
-/-
medaka larvae fed for 10 d (10th day of feeding) still had incompletely digested brine shrimp (
Artemia nauplii
) in the digestive tract 8 h after feeding
the messenger RNA (mRNA) expression levels of digestion-related genes (
amy
lpl
ctra
and
ctrb
) were significantly decreased
and the activity of amylase
trypsin
and lipase also significantly decreased. The deletion of
npy8br
in medaka larvae inhibited the growth and significantly decrease
d the expression of growth-related genes (
gh
and
igf1
). Hematoxylin and eosin (H
&
E) sections of intestinal tissue showed that
npy8br
-/-
medaka larvae had damaged intestine
thinned intestinal wall
and shortened intestinal villi. So far
this is the first
npy8br
gene knockout model established in fish and the first demonstration that
npy8br
plays an important role in digestion.
关键词
神经肽Y受体Y8（npy8br）日本青鳉(Oryzias latipes)敲除摄食消化
Keywords
Neuropeptide Y receptor Y8b (npy8br)Japanese medaka (Oryzias latipes)KnockoutFeedingDigestion
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