Novel insights into cell cycle regulation of cell fate determination

Novel insights into cell cycle regulation of cell fate determination 细胞周期调控细胞命运决定的新见解 1 first-author 1 2 3 corresp ‡Corresponding Author ‡Corresponding Author †E-mail:liuf@ioz.ac.cn †E-mail:liuf@ioz.ac.cn liuf@ioz.ac.cn State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China University of Chinese Academy of Sciences, Beijing 100049, China The stem/progenitor cell has long been regarded as a central cell type in development, homeostasis, and regeneration, largely owing to its robust self-renewal and multilineage differentiation abilities. The balance between self-renewal and stem/progenitor cell differentiation requires the coordinated regulation of cell cycle progression and cell fate determination. Extensive studies have demonstrated that cell cycle states determine cell fates, because cells in different cell cycle states are characterized by distinct molecular features and functional outputs. Recent advances in high-resolution epigenome profiling, single-cell transcriptomics, and cell cycle reporter systems have provided novel insights into the cell cycle regulation of cell fate determination. Here, we review recent advances in cell cycle-dependent cell fate determination and functional heterogeneity, and the application of cell cycle manipulation for cell fate conversion. These findings will provide insight into our understanding of cell cycle regulation of cell fate determination in this field, and may facilitate its potential application in translational medicine. 长期以来,干/祖细胞由于其具备的自我更新和多谱系分化能力,因而被视为生物体发育、稳态和再生过程中的一类重要细胞类型.干/祖细胞自我更新和多谱系分化之间的平衡是由细胞周期进程和细胞命运决定之间的协同调控来完成的.大量研究表明细胞周期状态可以决定细胞的命运,体现在处于不同细胞周期状态的细胞具有不同的分子特征和功能.目前,随着高分辨率的表观基因组学、单细胞转录组学和细胞周期实时标记系统的开发,我们对细胞周期如何调控细胞命运有了新的认识.本文总结了细胞周期调控细胞命运决定和功能异质性的分子机制,以及通过操纵细胞周期进而影响细胞命运转变的研究进展.这些发现将加深我们对细胞周期调控细胞命运决定机制的理解,同时也能促进其在转化医学中的潜在应用. Cell cycle Cell fate Heterogeneity Fate conversion Stem/progenitor cell 细胞周期 细胞命运 异质性 命运转变 干/祖细胞 Introduction 1 Cell proliferation is one of the fundamental biological activities that take place during development, homeostasis, and regeneration (Matson and Cook, 2017 2017 ). The sequence of stages during the cell proliferation process is generally termed the cell cycle, and is divided into a synthesis phase (S) and a mitotic segregation phase (M), with two intervenient gap phases (G1 and G2) preceding the S and M phases (Orford and Scadden, 2008 2008 ). The progression of the G1 phase is tightly regulated by a “start point” (in yeast) or a “restriction point” (in mammals) (Johnson and Skotheim, 2013 2013 ), which separates the G1 phase into early and late phases. A combination of intrinsic factors, such as the rate of protein synthesis, and extrinsic factors, such as mitogenic signals (growth factors, etc.), determines whether cells enter the cell cycle by passing through the “restriction point” (Zetterberg et al., 1995 1995 ). However, the absence of these essential factors results in cells exiting the cell cycle and entering a well-defined quiescent state known as the G0 phase. Cells already entering the cell cycle are further controlled by three “checkpoints”: the G1/S, G2/M, and mitotic spindle checkpoints (Hartwell and Weinert, 1989 1989 ; Pietenpol and Stewart, 2002 2002 ; Barnum and O'Connell, 2014 2014 ). At the molecular level, there is a growing body of evidence that cell cycle progression in eukaryotes is driven by an evolutionarily conserved central mechanism comprising cyclin-dependent kinases (CDKs), which are serine/threonine protein kinases that promote DNA synthesis and chromosome segregation by phosphorylating key targets. However, the catalytic activity of CDKs may be negatively regulated by CDK inhibitors (CKIs) (Lim and Kaldis, 2013 2013 ; Barnum and O'Connell, 2014 2014 ). According to their evolutionary origins, structures, and CDK specificities, two CKI gene families have been defined: the INK4 (inhibitors of CDK4/CDK6) family and the Cip/Kip family. The INK4 family includes p15 INK4b , p16 INK4a , p18 INK4c , and p19 INK4d ; and the Cip/Kip family includes p21 Waf1/Cip1 (p21, encoded by cdkn1a ), p27 Kip1 (p27, encoded by cdkn1b ), and p57 Kip2 (p57, encoded by cdkn1c ) (Harper et al., 1993 1993 ; Polyak et al., 1994 1994 ; Toyoshima and Hunter, 1994 1994 ; Lee et al., 1995 1995 ; Matsuoka et al., 1995 1995 ; Sherr and Roberts, 1999 1999 ; Besson et al., 2008 2008 ). The correct cooperation between cyclin, CDK, and CKI is critical to ensure the ordered progression through an intact cell cycle. Fate determination of stem/progenitor cells is strictly orchestrated by extrinsic signals and intrinsic regulators. Emerging evidence suggests that changes in cell cycle states affect the fate determination of stem/progenitor cells (Roccio et al., 2013 2013 ; Lauridsen et al., 2018 2018 ; Lu et al., 2018 2018 ). For example, previous studies on mammalian embryonic stem cells (ESCs) have demonstrated that cells in the G1 phase exhibit increased differentiation properties (Maimets et al., 2008 2008 ; Koledova et al., 2010 2010 ; Sela et al., 2012 2012 ), and cells in distinct stages of the G1 phase generate different germ layer cells (Dalton, 2013 2013 ; Pauklin and Vallier, 2013) (Fig. 1 1 ). These findings reveal that stem/progenitor cells stimulated by extrinsic signals remodel their cell cycle states—for example by changing the phase duration or arresting cycling progression—to activate intrinsically instructive signaling pathways (Lange et al., 2009 2009 ; Salomoni and Calegari, 2010 2010 ; Calder et al., 2013 2013 ; Dalton, 2015 2015 ; Mende et al., 2015 2015 ). In the early G1 phase, the TGF-β-SMAD2/3 pathway promotes the differentiation of ESCs into endodermal cells by activating endodermal genes, whereas the cyclin D-CDK4/6 complex governs the fate conversion of ESC-derived progenies from endoderm cells to neuroectoderm cells by phosphorylating SMAD2/3 in the late G1 phase. Once the cell cycle of ESCs is arrested in the S and G2 phases, DNA damage checkpoint factors ATM/ATR are activated, and subsequently stimulate p53 and cyclin B. Activated cyclin B enhances TGF-β/activin/nodal signaling, which can trigger the selective preference of pluripotency in ESCs. ESCs: embryonic stem cells; TGF-β: transforming growth factor-β; CDK: cyclin-dependent kinase; ATM: ataxia telangiectasia mutated; ATR: ATM and Rad3-related; CHEK2: checkpoint kinase 2 In the present review, we discuss recent advances in genomics profiling techniques and cell cycle reporter systems, with a focus on the cell cycle regulation of cell fate determination. First, we introduce the molecular mechanisms of cell cycle-dependent fate determination. We then describe the cell cycle regulation of cellular functional heterogeneity. Finally, we propose that cell cycle-dependent fate conversion may serve as a potential strategy for the treatment of cells with malignant transformations. Molecular mechanisms of cell cycle-dependent fate determination 1 Given that cell fate determination is intrinsically associated with cell cycle regulation (Dalton, 2015 2015 ; Vallier, 2015 2015 ), it is essential to fully understand the molecular features of cell cycle dynamics. To investigate cell cycle dynamics, chemical drugs have been widely employed to synchronize the cell cycle progression of various cells; however, such drugs can have side effects on cell differentiation (Pauklin and Vallier, 2013 2013 ). With the help of spatial-temporal observation systems for cell cycle progression (Sakaue-Sawano et al., 2008 2008 ; Coronado et al., 2013 2013 ; Zerjatke et al., 2017 2017 ), it is feasible to separate cells of interest into subpopulations with different cell cycle states, and to characterize their native properties. Furthermore, with the prevalent application of single-cell transcriptomics, there is emerging evidence that the cell cycle states of individual cells contribute to gene expression variation, and cell cycle-related genes also have an impact on cell type/subtype identification (Buettner et al., 2015 2015 ; McDavid et al., 2016 2016 ; Skinner et al., 2016 2016 ; Sun et al., 2017 2017 ; Lauridsen et al., 2018 2018 ). Moreover, several studies have also shown that developmental cells are characterized by different cell cycle states (Su et al., 2018 2018 ; Lu et al., 2019 2019 ). Overall, the revolution in technologies related to the cell cycle has boosted our understanding of cell cycle dynamics at the molecular level. G1 phase-dependent cell fate determination 2 G1 is crucial for cell fate determination, as reflected by the fact that stem/progenitor cells are particularly susceptible to differentiation signals in that phase. For instance, by employing the fluorescent ubiquitination-based cell cycle indicator (FUCCI) system to monitor the cell cycle states of human ESCs (hESCs), it has been revealed that ESCs respond to differentiation signals primarily occurring during a narrow window of the early G1 phase, whereas these cells remain insensitive during other cell cycle phases. Upon stimulation by SMAD2/3 transcription factors (TFs), hESCs differentiate into endodermal cells. In contrast, hESCs are converted into neuroectodermal cells following overexpression of the cyclin D-CDK4/6 complex. Mechanistically, the cyclin D-CDK4/6 complex inhibits the transcriptional activity of SMAD2/3 during the late G1 phase, thereby ensuring the fate conversion of hESC-derived progenies from endodermal to neuroectodermal cells (Pauklin and Vallier, 2013 2013 ). This result demonstrates that stem cells initiate fate determination via activation of cell cycle-regulated instructive factors in the G1 phase (Dalton, 2013 2013 , 2015 2015 ). Moreover, accumulating evidence suggests that a transient high expression of TFs, such as GATA6 and SOX17, in response to differentiation signals also occurs in the G1 phase in hESCs, and this transcriptional regulation is a major contributor to heterogeneity in those cells (Singh et al., 2013 2013 ). Furthermore, the transition from the M phase to the next G1 phase is associated with a dynamic change in the epigenetic landscape, involving such factors as chromosomal architecture (Thomson et al., 2004 2004 ; Dalton, 2015 2015 ), histone modification (Singh et al., 2013 2013 , 2015 2015 ; Gonzales et al., 2015 2015 ), and DNA methylation (Singh et al., 2013 2013 ; Ma et al., 2015 2015 ). Specifically, the epigenetic modification of 5-hydroxymethylcytosine (5hmC) peaks in the G1 phase and subsequently declines in the S phase. The 5-methylcytosine (5mC)/5hmC ratio during cell cycle progression may dictate active transcription in the G1 phase (Singh et al., 2013 2013 ). Notably, the cell cycle-dynamics of chromosomal organization have been profiled at single-cell resolution using high-resolution chromosome conformation capture techniques (Nagano et al., 2013 2013 ). It has been proposed that cell cycle progression makes a major contribution to chromosomal dynamics, and together with the accompanying gene regulatory network may be a prerequisite for cell fate determination (Nagano et al., 2017 2017 ) (Fig. 2 2 ). Taken together, these findings demonstrate that the G1 phase serves as a special window that enables the genetic/epigenetic regulation of cell fate-related genes to initiate the process of cell fate determination. (a) A schematic model showing the dynamics of chromosomal architecture during the cell cycle. (b) The potential mechanisms of cell cycle-dependent fate determination. Cell cycle-specific machinery, cooperating with epigenetic and genetic regulators, can directly orchestrate the cell fate determination of stem/progenitor cells. CDK: cyclin-dependent kinase G1 phase-independent cell fate determination 2 During cell differentiation, stem/progenitor cells experience various biological events, such as DNA damage, chromatin remodeling, and checkpoint activation, which lead to the downregulation of signaling pathways associated with pluripotency and the upregulation of differentiation-signaling pathways (Singh et al., 2013 2013 ; Akdemir et al., 2014 2014 ; Gonzales et al., 2015 2015 ). In addition to the role of the G1 phase in regulating stem/progenitor cell fate determination, the regulatory mechanisms of the S and G2 phases in such cell fate determination have also been gradually decoded. Systematic genomics studies have greatly advanced our knowledge of the regulatory network involved in hESC differentiation (Chia et al., 2010 2010 ). High-throughput RNA interference (RNAi) screening combined with small-molecule inhibitor treatment has revealed that the S and G2 phases have an intrinsic propensity to rapidly attenuate pluripotency in hESCs. Particularly when progression of the hESC S and G2 phases is perturbed, the DNA damage checkpoint factors ataxia telangiectasia mutated (ATM)/ATM and Rad3-related (ATR) stimulate the activity of p53/cyclin B, and subsequently enhance transforming growth factor-β (TGF-β)/activin/nodal signaling, which can trigger a selective preference for pluripotency (Betschinger et al., 2013 2013 ; Gonzales et al., 2015 2015 ) (Fig. 1 1 ). Taken together, these studies demonstrate that stem/progenitor cells in the G1 phase respond sensitively to differentiation signals, and subsequently lose their pluripotency in the S and G2 phases, indicating that stem/progenitor cells initiate cell fate determination in the G1 phase while committing to a specified fate in the S and G2 phases (Vallier, 2015 2015 ). Dynamic changes to epigenetic modification, such as chromatin remodeling, also occur in the S and M phases (Fig. 2 2 ), and may play a role in cell fate determination. Two essential cell cycle events occur in the S and M phases, and result in chromatin remodeling: first, new DNA synthesized in the S phase is assembled with newly synthesized histones to re-establish chromatin and the corresponding epigenetic modifications; second, the loose chromatin is condensed into chromosomes in the M phase, numerous chromatin-remodeling complexes and transcriptional complexes dissociate from the chromosome, and the nuclear envelope ultimately decomposes (Ma et al., 2015 2015 ). Thus, histone acetylation, nucleosome remodeling, and widespread DNA demethylation, which take place during the S and M phases, contribute to the tightly regulated processes of cell fate determination (Singh et al., 2013 2013 ; Gonzales et al., 2015 2015 ). Cell cycle regulation of functional heterogeneity 1 Although the study of molecular mechanisms can help elucidate the interplay between cell cycle regulators and cell fate determinants, it is necessary to link molecular mechanisms to cellular functions in order to understand the contribution made by the cellular state to cell heterogeneity during development and homeostasis (Lein et al., 2017 2017 ; Haas et al., 2018 2018 ). For example, it has been demonstrated that quiescent and cycling stem/progenitor cells are distinguished by their metabolic states, morphologies, and differentiation outputs (Ohnuma and Harris, 2003 2003 ; Li and Clevers, 2010 2010 ; Lugert et al., 2010 2010 ; Salazar-Roa and Malumbres, 2017 2017 ; Haas et al., 2018 2018 ). In particular, quiescent mammalian hematopoietic stem cells (HSCs) have more robust long-term reconstitution ability in blood system-impaired recipients than cycling HSCs (Haug et al., 2008 2008 ; Li and Clevers, 2010 2010 ; Lauridsen et al., 2018 2018 ). This ability is associated with the low reactive oxygen species production, low molecular synthesis rate, and inactive metabolic state of HSCs (Ito and Suda, 2014 2014 ; Walter et al., 2015 2015 ; Qian et al., 2016 2016 ). Moreover, a recent study of Drosophila neural stem cells (NSCs) revealed that quiescent stem cells can be arrested in the G2 phase, as well as in the G0 phase, which is well known (Cheung and Rando, 2013 2013 ). Once stimulated by insulin signals, quiescent NSCs in the G2 phase re-enter the cell cycle before quiescent NSCs in the G0 phase. Functionally, this unexpected phenomenon may ensure the orderly development of neurons into appropriate neural circuits (Otsuki and Brand, 2018 2018 ). These results demonstrate that heterogeneous cell cycle states lead to differences in cellular function (Pauklin and Vallier, 2013 2013 ; Gonzales et al., 2015 2015 ; Vallier, 2015 2015 ; Gruenheit et al., 2018 2018 ). Cell cycle speed is also associated with differentiation outputs of mouse HSCs and progenitor cells (Lu et al., 2018 2018 ; Upadhaya et al., 2018 2018 ). These studies indicate that disparate cell cycle speeds may also serve as an essential determinant of cellular functional output. Taken together, heterogenous cell cycle states—such as quiescence versus cycling, G0 versus G2, and early G1 versus late G1—and disparate cell cycle speeds contribute to the functional heterogeneity of adult stem/progenitor cells. Cell fate conversion by manipulating cell cycle 1 Cell fate conversion through defined factors provides a novel strategy for tissue/organ regeneration and disease treatment (Lis et al., 2017 2017 ; Liu et al., 2017 2017 ). During development, TFs play a crucial role in the generation and maintenance of cells with distinct fates (Gurdon, 2016 2016 ). Several researchers have accomplished direct fate conversion by manipulating the master TFs in hematopoietic and nervous systems (Xie et al., 2004 2004 ; Vierbuchen et al., 2010 2010 ; Zhang et al., 2018 2018 ). As mentioned above, cell cycle regulators also participate in fate determination by remodeling cell cycle states or cooperating with TFs. Studies on pre-artery/artery specification have revealed that the reduced expression of G1 phase-specific genes resulting from changes in blood flow is required for the expression of artery-generating genes. Specifically, the Notch-GJA4-CKI and chicken ovalbumin upstream promoter transcription factor 2 (COUP-TF 2) signaling pathways are involved in the cycle regulation of pre-artery/artery specification (Fang et al., 2017 2017 ; Su et al., 2018 2018 ). Arresting the cell cycle in the G1 phase combined with p53 suppression can markedly improve the efficiency of fate conversion from human fibroblasts to dopaminergic neurons when mediated by TFs and microRNA. Mechanistically, highly efficient fate conversion requires the overexpression of epigenetic regulator Tet1 , and cell cycle-mediated remodeling of cellular states is essential for overcoming the fate conversion barrier (Jiang et al., 2015 2015 ). Fate conversion by manipulating the cell cycle has now been accomplished in several cell types, indicating its potential application in translational medicine and disease modeling. Concluding remarks 1 The development of high-resolution epigenome profiling techniques, single-cell transcriptomics, and cell cycle reporter systems has resulted in a better understanding of the relationships between molecular features, cell cycle progression, and fate determination (Fig. 3 3 ). At the molecular level, when cyclin-CDK/CKI complexes are coordinated with master TFs, they are capable of determining the fate of a cell. Specifically, cell fate determination occurs in the early G1 phase, and the initiation of cell differentiation takes place in the S and G2 phases. When responding to differentiation signals, stem/progenitor cells execute cell fate determination primarily in the early G1 phase owing to transcriptional restart and chromatin reorganization. Subsequently, stem/progenitor cells lose their pluripotency and commit to a specified fate in the S and G2 phases, when cells establish new chromatin/epigenetic modifications and undergo dramatic changes to their nuclear architectures. Furthermore, stem/progenitor cells in various cell cycle states—such as quiescent cells versus cycling cells, G0 quiescent cells versus G2 quiescent cells, and early G1 cells versus late G1 cells—are distinguished by their morphologies, metabolic states, self-renewal abilities, and differentiation outputs. Cell cycle speed and phase duration can also contribute to the functional heterogeneity of stem/progenitor cells. Although the cell cycle serves as an essential fate determinant, caution is required when analyzing cell cycle information from transcriptomics data (Sun et al., 2017 2017 ). Cell fate determination is attributed to epigenetic landscapes, transcription states, and the cell cycle states of stem/progenitor cells. Epigenomic analysis techniques, such as single-cell Hi-C (high-resolution chromosome conformation capture), can capture the chromosomal conformation of individual cells during the cell cycle. Transcriptomic analyses can reveal the transcription state during the cell cycle. Cell cycle reporter systems can dynamically trace cell cycle progression by live imaging Currently, studies on cell cycle-dependent cell fate determination are based on cell cycle perturbation. Therefore, the regulatory mechanisms underlying normal cell cycle progression in cell fate determination remain largely unknown. Furthermore, there is no comprehensive framework for the integrative analysis of the cell cycle dynamics of chromosomal architecture, epigenetic modification, or gene expression during cell fate determination (Nagano et al., 2017 2017 ). The establishment of a comprehensive framework would enable the elucidation of the molecular mechanisms underlying cell cycle-dependent fate determination on a genome-wide scale. Furthermore, using cell cycle reporter systems, it is now feasible to dynamically trace cell cycle progression. It would be helpful if future studies focused on the real-time visualization of the cell cycle dynamics of various fate-determined regulators at single-cell or subcellular resolution. However, it is still unclear whether the mechanisms identified in ESCs in vitro are conserved in the cell fate determination of adult stem/progenitor cells in vivo. Importantly, the direct targeting of cell cycle regulators by small molecules or by genetic perturbation has produced favorable results with regard to the suppression of pathologic neuron degeneration and tumorigenesis. However, these experiments were performed in vitro in mammalian tumor cell lines, or in vivo using genetically engineered disease models (Kar et al., 2006 2006 ; Chatterjee et al., 2016 2016 ; Biswas et al., 2017 2017 ; Tomás-Loba et al., 2019 2019 ). Therefore, the translation of basic research into clinical treatment remains a critical challenge. Addressing the questions mentioned above will promote our understanding of cell cycle regulation in cell fate determination, and may facilitate its application in translational medicine. Glossary 2 ESC (embryonic stem cell): a totipotent stem cell derived from the inner cell mass of a blastocyst that can differentiate into three germ layers and produce any type of cell within the embryo. HSC (hematopoietic stem cell): a multipotent stem cell residing in adult bone marrow that can yield all mature blood cells, including myeloid, lymphoid, and erythroid cells. NSC (neural stem cell): a multipotent stem cell residing in the hippocampal dentate gyrus of the adult brain that can differentiate into neurons, astrocytes, and oligodendrocytes. FUCCI (fluorescent ubiquitination-based cell cycle indicator): a reporter system that can be used to monitor the phase transition of the cell cycle in living cells. 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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes The study was also supported by the State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Compliance with ethics guidelines Su-wei GAO and Feng LIU declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects performed by either of the authors. 10.1631/jzus.B1900197 Project supported by the Ministry of Science and Technology of China (No. 2016YFA0100500), the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA16010207), and the National Natural Science Foundation of China (Nos. 31425016, 81530004, and 31830061) 2019-04-13 2019-04-24 2022-01-05 Copyright © Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2019 2019 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 06 20