Bamboo leaf extract improves spatial learning ability in a rat model with senile dementia

Bamboo leaf extract improves spatial learning ability in a rat model with senile dementia 竹叶提取物改善老年痴呆模型大鼠的空间学习能力 first-author corresp ‡Corresponding Author ‡Corresponding Author †E-mail:dingyuemin@zucc.edu.cn †E-mail:dingyuemin@zucc.edu.cn dingyuemin@zucc.edu.cn Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou 310015, China Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou 310015, China Senile dementia (SD) is a syndrome characterized by progressive neurological deterioration. Treatment for the disease is still under investigation. Bamboo leaf extract (B-extract) has been known for its biological efficacy in anti-oxidant and anti-cancer activities. However, study on B-extract for its protection against dementia is very limited. The effect of B-extract on a rat model with SD was examined. B-extract improved spatial learning ability of the dementia rats. The hippocampus of dementia model rats showed reduced levels of acetylcholine (ACh), epinephrine (E), norepinephrine (NE), and dopamine (DA), and increased activities of acetylcholine esterase (AChE) and monoamine oxidase (MAO). Treatment with B-extract 20 mg/(kg·d) for 7 weeks significantly inhibited the enzyme activity compared with untreated dementia rats, and raised the levels of ACh, E, and DA in the hippocampus. In addition, treatment with B-extract elevated the level of γ-aminobutyric acid (GABA), but reduced the level of glutamate (Glu) in the brain. These data suggest that B-extract might be a potential drug in treating impairment of spatial memory in dementia rats by regulating the central neurotransmitter function. 目的 2 竹叶提取物在抗氧化和抗肿瘤方面的生物学效应已经为人所知，但是它是否能防治痴呆还未见研究报道。本课题旨在研究竹叶提取物对老年痴呆大鼠的空间学习能力的影响及其可能机制。 创新点 2 本研究首次提出竹叶提取物能显著改善老年痴呆大鼠的空间学习能力，它能通过抑制胆碱酯酶和单胺氧化酶的活性来增加海马组织乙酰胆碱和单胺类递质的含量，并且调控谷氨酸和γ-氨基丁酸的含量，从而正面影响老年痴呆大鼠的神经功能。 方法 2 采用每日腹腔注射D-半乳糖（60mg/kg），6周末于侧脑室注射β-淀粉样物（Aβ）l-42（10µg）的方法制造老年痴呆大鼠模型。治疗组大鼠在造模过程中连续7周每日喂饲竹叶提取物（20mg/kg）。7周末用Morris水迷宫进行为期5天的定位航行试验，结束后取海马和皮质脑组织，用酶联免疫吸附测定法（ELISA）测定乙酰胆碱、单胺类递质、5-羟色胺、谷氨酸和γ-氨基丁酸含量及相关酶活性。 结论 2 竹叶提取物可能在改善老年痴呆大鼠空间记忆能力方面有潜在治疗价值，其作用可能通过调控脑内神经递质功能得以实现。 Bamboo leaf extract Dementia Alzheimer’s disease Neurotransmitter 竹叶提取物 痴呆 阿尔茨海默病 神经递质 Introduction 1 Senile dementia (SD) causes long term deficit in memory and cognition that is severe enough to affect a person’s daily functioning (Sharma and Singh, 2010 2010 ). It is reported that in China the number of people with dementia is 6–7 million with an incidence of 5%–7% of people over 65 years of age (Song and Wang, 2010 2010 ). Alzheimer’s disease (AD) is the most common type of dementia. It is estimated that there will be more than 80 million AD patients in the world by 2040 (Ferri et al., 2005 2005 ) and it has become the fourth largest fatal disease (Hoyert and Xu, 2012 2012 ). Treatment costs of AD patients amount to 604 billion US dollars per year in the world (Wimo et al., 2013 2013 ). Such huge costs have caused a great impact on social and economic development. However, at present, the etiology and disease progression of AD is not fully understood. The main hypotheses are as follows: apoptosis of the central cholinergic neurons and reduction of cholinergic neurotransmitters (Xie et al., 2006 2006 ; Wang and Ding, 2008 2008 ; Checler and Turner, 2012 2012 ), aggregation and deposition of amyloid-β (Aβ) (Hardy, 2006 2006 ), abnormal phosphorylation of tau protein (Lewczuk et al., 2004 2004 ; Iqbal et al., 2005 2005 ; Chun and Johnson, 2007 2007 ), and formation of a neurofibrillary tangle. In addition, theories such as neurotoxicity of the excitatory neurotransmitter, oxidative stress, and mitochondrial damage can also explain the causes of dementia to some extent and provide a new theoretical basis and therapeutic target for the development of novel drugs for AD (Tamagno et al., 2006 2006 ; Liu et al., 2010 2010 ). Current medications commercially available for the treatment of AD can improve cognitive ability and daily behavior of AD patients (Deguil et al., 2013 2013 ). However, these drugs can only alleviate the symptoms of patients instead of terminating or fundamentally reversing the dementia process (Wermuth, 2004 2004 ). Bamboo leaf is used to treat many diseases in Chinese traditional medicine. In recent years, studies have found that bamboo leaf extract (B-extract) has excellent biological efficacy in anti-oxidant activity, anti-aging activity, resistance to free-radical, and cardiovascular protection (Lee et al., 2008 2008 ; Choi et al., 2013 2013 ). Theoretically, these functions would have a therapeutic effect for AD. However, study on B-extract for its protection against neurodegenerative diseases and resistance to dementia is very limited. In this project, a rat model with SD was obtained by intracerebroventricular injection of amyloid peptide after chronic intraperitoneal injections of D -galactose ( D -gal) for 6 weeks (Wei et al., 2005 2005 ). During the period, the rats were fed with B-extract via gastric gavage for 7 consecutive weeks. Evaluation of behavioral and biochemical parameters was conducted to systematically study the effect of B-extract on the spatial cognitive abilities of dementia model rats and the underlying mechanisms. Materials and methods 1 Ethics statement 2 The experimental procedures were approved by the Animal Ethics Committee of Zhejiang University (Hangzhou, China) and were carried out in accordance with institutional guidelines. All efforts were made to minimize the number of animals used and their suffering. Reagents 2 B-extract was purchased from Johncan Bio-technology (Hangzhou, China). D -gal was purchased from Changqing Chemical (Hangzhou, China). Aβ 1–42 was purchased from GL Biochem (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) kits for acetylcholine (ACh), acetylcholine esterase (AChE), choline acetyltransferase (ChAT), monoamine oxidase (MAO), dopamine (DA), epinephrine (E), norepinephrine (NE), 5-hydroxy tryptamine (5-HT), glutamate (Glu), and γ-aminobutyric acid (GABA) were purchased from Jingtian Bio-Tech (Shanghai, China). Animal groups 2 Forty-five male Sprague Dawley rats (200±20) g), purchased from SLAC Laboratory (Shanghai, China), were randomly divided into three groups: control group, SD model group (SD), and bamboo leaf extract treatment group (B-extract+SD), with 15 rats in each group. The rats in the SD and B-extract+SD groups were injected intraperitoneally with D -gal (60 mg/(kg·d)) during the first 6 weeks to induce aging models and the rats in the control group were injected with the same volume of saline. At the same time, the rats in the treatment group were fed with B-extract (20 mg/(kg·d)) via gastric gavage, while the rats in the control group and the SD group were fed with equal amounts of physiological saline. At the end of the 6th week, the rats in the SD and B-extract+SD groups were anaesthetized with intraperitoneal injection of 2% (0.02 g/ml) of pentobarbital sodium (80 mg/kg). A stereotaxic apparatus (Stoelting, USA) was used to accurately position right lateral ventricle (1.0 mm behind anterior fontanelle, 1.5 mm right to raphe, and 3.5 mm under cortical surface), into which 5 μl of Aβ 1–42 (2 μg/μl) was slowly injected (Lamberty et al., 1992 1992 ). After that, the rats were fed with B-extract or saline by gastric gavage continuously for one more week before behavioral testing (Fig. 1 1 ). i.p.: intraperitoneal injection; i.c.v.: intracerebroventricular injection Morris water maze test 2 After seven weeks of B-extract treatment, all animals were tested for place navigation with the Morris water maze (RWD Life Science, Shenzhen, China) for five consecutive days. The rats were put into the water four times a day from different quadrants. The time that a rat took to find the platform (escape latency) was recorded. The experimental process was recorded by video and analysis software of small animal behavior (SMART 2.5, Harvard, USA) was used to determine escape latency. Neurotransmitter detection 2 Immediately after behavioral testing, the animals were anaesthetized and decapitated. Hippocampus and cortical tissues of the rats were quickly dissected out and homogenized with ultrasound and diluted with saline. The ELISA method was applied to determine the biochemical parameters and neurotransmitter content. The operation was performed according to the instructions of the kit. Briefly, the supernatant was taken out after centrifugalization at 12 000 r/min (4 °C) for 30 min, and the contents of ACh, DA, E, NE, 5-HT, Glu, and GABA as well as the activities of AChE, ChAT, and MAO were determined. Statistical analysis 2 Data were expressed as mean±standard error of the mean (SEM) and analyzed using SPSS 13.0 statistical software (SPSS, Chicago, IL, USA). Comparisons between groups were analyzed by two-way analysis of variance (ANOVA) or one-way ANOVA and Dunnett’s test. A level of P < 0.05 was considered statistically significant. Results 1 Effects of B-extract on spatial learning and memory of dementia rats 2 A rat model with SD was established and the dementia rats were fed with B-extract for 7 weeks. At the end of the 7th week, the rats were tested for spatial learning and memory with a 5-d Morris water navigation task. Subsequently, the average escape latency and path length between the point of origin and platform were measured every day (Fig. 2 2 ). As a result, the difference of the average latency and path length among the three groups on the first day of the test was not statistically significant ( P > 0.05). After training for two days, average escape latency and path length of the rats in SD group were longer than those of the rats in control group ( P < 0.05), suggesting that the rats in SD group had worse spatial memories. Compared with the rats in SD group, from Day 2, both average escape latency and path length of the rats in B-extract+SD group were significantly shortened ( P < 0.05), which indicated that B-extract could improve spatial memory ability of dementia rats. Average escape latency (a) and path length (b) of the dementia rats were measured with a Morris water maze. Data are expressed as mean±SEM ( n =15). * P < 0.05, ** P < 0.01 vs. control group; # P < 0.05, ## P < 0.01 vs. SD group. (c) Representative swim paths of the animals. The black circle represents the platform Effect of B-extract on the content of ACh, and the activities of AChE and ChAT in brain tissue 2 In order to clarify the possible mechanisms of B-extract improving the spatial memory ability of dementia rats, we determined the content of neurotransmitters, which might affect memory, and the activities of key enzymes to regulate the synthesis in the hippocampus and cerebral cortex in the brain. Fig. 3 3 shows the determination of ACh content, ChAT activity, and AChE activity in the hippocampus and cerebral cortex of the rats. We found that the content of ACh in the hippocampus and cerebral cortex of rats in SD group is lower than that in the control group ( P < 0.05). B-extract treatment, as expected, increases the ACh content in the hippocampus ( P < 0.05). However, the difference in ChAT activity among the groups has no statistical significance ( P > 0.05). Compared with the control group, AChE activity in the hippocampus and cerebral cortex of the rats in the SD group increases significantly ( P < 0.001) and AChE activity in the hippocampus and cerebral cortex of the rats in B-extract+SD group is significantly lower than that in SD group ( P < 0.05 and P < 0.001, respectively). Level of the total acetylcholine (ACh) content (a), activity of choline acetytransferase (ChAT) (b), and activity of acetylcholine esterase (AChE) (c) in the hippocampus and cortex of rats ( n =8). Data are expressed as mean±SEM. * P < 0.05, *** P < 0.001 vs. control group; # P < 0.05, ### P < 0.001 vs. SD group Effect of B-extract on the contents of E, NE, and DA, and the activity of MAO in brain tissue 2 Next we determined monoamine neurotransmitters and the activity of their key enzymes. The contents of E, NE, and DA in the hippocampus of the rats in SD group are significantly lower than those in the control group ( P < 0.05), and the activity of MAO in the hippocampus of the rats in the SD group is significantly higher than that in the control group ( P < 0.05). However, no significant change was observed in cortical tissue except for the DA content (Fig. 4 4 ). MAO catalyzes the deamination reaction, which is the main pathway to remove the monoamine neurotransmitters by enzymatic oxidation. Hence the decrease in monoamine neurotransmitters in the hippocampus of the rats in the SD group may be associated with increased MAO activity. At the same time, the contents of E and DA in the hippocampus of the rats in the B-extract+SD group are significantly higher than those in SD group ( P < 0.001 and P < 0.05, respectively), and MAO activity is lower than that in the SD group ( P < 0.05). It can be concluded that B-extract could increase the content of monoamine neurotransmitters in the hippocampus by reducing the activity of MAO. The contents of the total epinephrine (E) (a), norepinephrine (NE) (b), and dopamine (DA) (c), and activity of monoamine oxidase (MAO) (d) in the hippocampus and cortex of rats ( n =8). Data are expressed as mean±SEM. * P < 0.05, ** P < 0.01 vs. control group; # P < 0.05, ### P < 0.001 vs. SD group Effect of B-extract on the contents of 5-HT, Glu, and GABA in brain tissue 2 In addition, we studied the content of several important excitatory and inhibitory neurotransmitters in the hippocampus and cortex. Compared with the control group, the contents of 5-HT, Glu, and GABA in the brain tissues of the rats in the SD group do not change significantly ( P > 0.05). After treatment with B-extract, however, the content of Glu in the hippocampus of the rats in the B-extract+SD group is reduced by 28.6% ( P < 0.05) when compared with that of the SD group, while the content of GABA is increased by 18.2% in the B-extract+SD group ( P < 0.05; Fig. 5 5 ). Similarly, B-extract treatment also elevated the content of GABA in the cortex by 15.4%. The contents of the total 5-hydroxy tryptamine (5-HT) (a), glutamate (Glu) (b), and γ-aminobutyric acid (GABA) (c) in the hippocampus and cortex of rats ( n =8). Data are expressed as mean±SEM. ** P < 0.01, *** P < 0.001 vs. control group; # P < 0.05 vs. SD group Discussion 1 With the advancement of understanding of the pathogenesis of AD, a series of animal models of dementia have been developed (Ribeiro et al., 2013 2013 ; Neha et al., 2014 2014 ). An ideal animal model should exhibit all AD features in animals (Neha et al., 2014 2014 ). Aging animals are commonly employed in AD drug development because they mimic not only the morphological characteristics (Erickson and Barnes, 2003 2003 ; Gupta et al., 2013 2013 ) but also the pathophysiology (de Souza Silva et al., 2013 2013 ) of human Alzheimer’s disease. Chronic administration of D -gal induces changes that resemble natural aging in rodents, including neurodegeneration (Ho et al., 2003 2003 ; Zhang et al., 2005 2005 ) and cognitive dysfunction (Cui et al., 2006 2006 ; Wang et al., 2013 2013 ), and thus becomes a routinely used method to induce aging in rodents (Wei et al., 2005 2005 ). In addition, Aβ plaques are a histopathological hallmark of AD and intrahippocampal infusion or intracerebroventricular injection of Aβ has been widely applied in animal models of dementia, especially Alzheimer’s disease (McLarnon and Ryu, 2008 2008 ; Lopes et al., 2010 2010 ). In the present study, in order to mimic the natural development of AD, we adopted a combined administration (Qin et al., 2012 2012 ) of D -gal and Aβ 1–42 to generate rat models with SD. The typical clinical symptoms of SD include cognitive decline as well as loss and gradual deterioration of recent memory. The Morris water navigation task is widely used to study spatial learning and memory. Here, we measured spatial learning and memory of the rats by analyzing average escape latency. According to the behavioral evaluation results, the average escape latency in the SD group was longer than that in the control group, which indicated the decline of spatial memory in the dementia model. Memory formation is associated with cholinergic neuronal function of the central nervous system, especially in the hippocampus and basal ganglia. ACh is necessary for memory formation and is the physiological basis for long-term memory. Increase in synthesis and release of ACh or decrease in its degradation may improve learning and memory ability (Singh et al., 2013 2013 ). The synthesis of ACh is directly associated with the activity of ChAT. Studies have reported that dementia is related to the decrease in ChAT activity in the brain (Yamaguchi et al., 2002 2002 ). In the present study, the content of ACh in both the hippocampus and cerebral cortex of the SD group was lower than that of the control group, while ChAT activity does not change significantly. It suggests that the main cause for the reduced content of ACh in the cholinergic neuron in dementia rats is not the decreased activity of ChAT. AChE is a hydrolase in the nervous system to degrade ACh and terminate related nerve impulse. Cholinergic deficit is an early finding in SD and AChE has been the most viable therapeutic target for dementia (Naaz et al., 2013 2013 ). In the clinic, controlled use of AChE inhibitors has proved valuable for the treatment of dementia (Singh et al., 2013 2013 ). Compared with the control group, AChE activity in the hippocampus and cerebral cortex of the rats in the SD group significantly increased while it can returned to a normal level after B-extract treatment. Taking into account that AChE degrades ACh, the decreased content of ACh in SD group is likely caused by accelerated decomposition. In addition, studies have shown that there is increasing AChE and Aβ in senile plaques of AD patients (Alvarez et al., 1997 1997 ). AChE and Aβ may combine to form a stable compound, which is more toxic to neurons, and finally result in neuronal apoptosis (Golde et al., 2011 2011 ). Therefore increased AChE activity in the brain of AD rats may produce a more toxic effect on neurons. In the present study, we found that B-extract treatment could reduce AChE activity to maintain ACh content in hippocampal neurons of the dementia rats. It suggests that B-extract improves the ability of learning and memory in dementia model rats by regulating cholinergic metabolism. Monoamine neurotransmitters are the most important neurotransmitters in the brain, and play an irreplaceable role in the physiological activities of central nervous system. MAO catalyzes oxidative deamination of monoamine neurotransmitters such as E, NE, and DA, and generates hydrogen peroxide (Mandel et al., 2007 2007 ; Hureau et al., 2010 2010 ). The latter will combine with metal ions in the brain of AD rats to generate reactive oxygen species (ROS) (Alper et al., 1999 1999 ) and cause Aβ deposition. Aggregation and deposition of Aβ will in turn result in the increase in ROS in cells, enhance oxidative stress, and finally kill neurons (Marchi et al., 2012 2012 ). Here we found that in the hippocampus, which is most closely related to learning and memory, the activity of MAO in SD group increased significantly, while the content of E, NE, and DA decreased. We hypothesize that the increased deamination of MAO produces large amounts of hydrogen peroxide to greatly activate oxidative stress downstream and result in injury of hippocampal neurons. Interestingly, B-extract also effectively reduces the activity of MAO and increases the content of monoamine neurotransmitters in the hippocampus. Therefore, a protective effect of B-extract on neurons may be achieved through reducing the deamination of MAO and inhibiting the oxidative stress reaction. Excitatory neurotransmitter Glu in the hippocampus may regulate the process of learning and memory through its different types of receptors and signal transduction pathways. They are essential neurotransmitters to maintain the function of the hippocampus (Peng et al., 2011 2011 ). High concentration of Glu, however, produces oxidative stress toxicity and causes excitatory injury of neurons. The content of Glu in the hippocampus and cortex was determined in this study. We reported that although the content of Glu in SD group did not increase remarkably, B-extract could significantly reduce its content. In addition, we also measured the content of 5-HT in the hippocampus and cortex and found little effect of B-extract on the content of 5-HT. Interestingly, when the inhibitory amino acid GABA was determined, there was a great increase in the content of GABA in the hippocampal and cortex tissues, but its mechanism needs further study. It should be mentioned that parts of our experimental findings varied in the hippocampus and cortex. It is possible that hippocampal neurons might be more vulnerable to toxicity induced by various insults than cortical neurons. Alternatively, the changes in the cortex were too small to be reliably detected by our methods. Conclusions 1 B-extract has been extensively studied for its anti-oxidant activity and anti-tumor effect. However, to the best of our knowledge, the present study is the first investigation on the preventive and therapeutic effect of B-extract in the context of a SD model. In summary, the present study demonstrated that B-extract improved the learning and memory ability of dementia model rats by regulating the activities of the key enzymes for the biosynthesis and degradation of neurotransmitters, changing the contents of cholinergic, monoamine, and amino acid neurotransmitters in the hippocampus. Thus, the B-extract showed significant therapeutic effects. However, the detailed mechanism of this function needs further investigation. References G Alper , FK Girgin , M Ozgonul , 等 . MAO inhibitors and oxidant stress in aging brain tissue . Eur Neuropsychopharmacol , 1999 . 9 ( 3 ): 247 - 252 . 10208295 DOI: 10.1016/S0924-977X(98)00035-2 http://doi.org/10.1016/S0924-977X(98)00035-2 . 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All institutional and national guidelines for the care and use of laboratory animals were followed. 10.1631/jzus.B1400249 Project supported by the Zhejiang Provincial Natural Science Foundation of China (No. LY14H090002) and the National Undergraduate Training Programs for Innovation and Entrepreneurship of China (No. 201313021016) 2014-09-20 2015-06-16 2015-02-01 2022-01-05 Copyright © Zhejiang University and Springer-Verlag Berlin Heidelberg 2015 2015 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 07 16