PGL3对水稻叶绿素合成和叶片衰老的影响

PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice # # PGL3 对水稻叶绿素合成和叶片衰老的影响 1 2 first-author 2 2 2 2 2 2 2 2 2 corresp ‡Corresponding Authors ‡Corresponding Authors †E-mail:fy_555500@163.comwangshusl@126.com †E-mail:fy_555500@163.comwangshusl@126.com fy_555500@163.com 1 corresp ‡Corresponding Authors ‡Corresponding Authors †E-mail:fy_555500@163.comwangshusl@126.com †E-mail:fy_555500@163.comwangshusl@126.com fy_555500@163.com College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China Rice leaf color mutants play a great role in research about the formation and development of chloroplasts and the genetic mechanism of the chlorophyll (Chl) metabolism pathway. pgl3 is a rice leaf color mutant derived from Xiushui11 ( Oryza sativa L. spp. japonica ), treated with ethyl methane sulfonate (EMS). The mutant exhibited a pale-green leaf ( pgl ) phenotype throughout the whole development as well as reduced grain quality. Map-based cloning of PGL3 revealed that it encodes the chloroplast signal recognition particle 43 kDa protein (cpSRP43). PGL3 affected the Chl synthesis by regulating the expression levels of the Chl synthesis-associated genes. Considerable reactive oxygen species were accumulated in the leaves of pgl3 , and the transcription levels of its scavenging genes were down-regulated, indicating that pgl3 can accelerate senescence. In addition, high temperatures could inhibit the plant’s growth and facilitate the process of senescence in pgl3 . 目的 2 研究 PGL3 的遗传机制与生物学功能. 创新点 2 研究一个水稻叶色突变体的鉴定与基因克隆,并探讨其对叶绿素合成和叶片衰老的影响. 方法 2 通过乙基甲磺酸(EMS)诱变,获得了一个淡绿叶突变体 pgl3 ,运用图位克隆法对 PGL3 进行定位,并对 PGL3 的功能进行研究. 结论 2 PGL3 通过调节叶绿素合成相关基因的表达水平影响叶绿素合成. pgl3 叶片中清除活性氧基因转录水平下调,导致活性氧大量积累,表明 pgl3 可以加速衰老.此外,高温可以抑制植物的生长并加速 pgl3 衰老. Pale-green leaf Chlorophyll synthesis Reactive oxygen species Senescence Rice 淡绿叶 叶绿素合成 活性氧 衰老 水稻 Introduction 1 The production yield is largely determined by the process of photosynthesis, which provides the raw materials for all plant products (Richards, 2000 2000 ). Chlorophyll (Chl) is pivotal to photosynthesis as it captures light energy, transforms it into chemical energy, and plays an important role in plant life activities (Lee et al., 2005 2005 ). For almost all plants, photosynthesis is the main energy source. Thus, increasing Chl content (Huang et al., 2013 2013 ) and extending the duration of photosynthesis (Long et al., 2006 2006 ) could substantially enlarge photosynthetic products, and thereby increase crop yields. Chl-deficient genes usually directly or indirectly affect chloroplast development and Chl metabolism, then alter Chl content and finally diversify the leaf color (Ramundo et al., 2014 2014 ). The considerable variation of Chl content regularly leads to the alteration of leaf color, so Chl-deficient mutation is the same as leaf color mutation (Zhang et al., 2013 2013 ). Leaf color mutants are very useful and are regarded as important genetic materials in the exploration of Chl synthesis and degradation mechanisms (Deng et al., 2014 2014 ). Leaf color mutants have very extensive sources and mainly originate from natural and artificial mutation. Leaf color mutants can be distinguished by their pale green, yellow, and albino color (Carol et al., 1999 1999 ). In recent years, more and more leaf color mutants have not only provided us many genetic materials, but also facilitated the understanding of the mechanisms of leaf color formation, chloroplast development, and Chl biosynthesis and degradation. Chl synthesis is a complex reaction process involving many enzymes in rice. OsGluRS encodes glutamyl-tRNA synthetase, which is the key enzyme in the initial step of Chl synthesis, and its mutant is characterized by yellow green leaves and is sensitive to temperature (Liu WZ et al., 2007 2007 ). OsChlH , OsChlI , and OsChlD encode H, I, and D subunits of magnesium chelatase, respectively, and any variation of them will cause the color to change (Goh et al., 2004 2004 ; Zhang et al., 2006 2006 ). OsDVR encodes divinyl reductase, which is essential for Chl biosynthesis, as well as its mutant exhibiting a yellow-green leaf phenotype, which reduces Chl level and arrests chloroplast development (Wang P et al., 2010 2010 , 2013 2013 ). A characteristic of leaf senescence is Chl degradation, which is not as complicated as Chl synthesis, but only a few related genes have been cloned up to now (Zhang et al., 2013 2013 ). NYC1 catalyzes Chl b to 7-hydroxymethyl Chl a and is necessary for the first step of Chl b degradation (Kusaba et al., 2007 2007 ). NOL , as the highly homologous gene of NYC1 , also encodes a Chl b reductase, and its mutant shows a stay-green phenotype and is very similar to the nyc1 mutant. NYC1 and NOL are localized on the thylakoid membrane and form a complex, which exercises the function of Chl b reductase (Sato et al., 2009 2009 ). In autumn, plant leaves generally change from green to yellow or red because of Chl degradation combined with carotenoid retention or anthocyanin accumulation, which is one of the important signs of leaf senescence (Park et al., 2007 2007 ). The slow Chl degradation in the senescence process can lead to a late-green phenotype, which can delay the senescence process, prolong the photosynthesis time, and increase the crop yield to some extent (Wu et al., 2012 2012 ). The process of senescence will produce large amounts of reactive oxygen species (ROS), mainly due to the process of cell death and cell membrane rupture (Piquery et al., 2000 2000 ). ROS control many different processes in plants, whereas, being toxic molecules, they are also capable of injuring cells (Mittler et al., 2004 2004 ). In this study, we characterized a rice mutant, pgl3 , which displayed pale-green leaves ( pgl ) and an insensitivity to heat and darkness treatments. Map-based cloning showed that PGL3 encodes a chloroplast signal recognition particle, OscpSRP43 . Here, we present the results of phenotypic and physiological characterizations and the expression analysis of associated genes, which indicate that PGL3 plays an important role in Chl synthesis and leaf senescence in rice. Materials and methods 1 Plant materials and growing conditions 2 The pgl3 mutant was derived from Xiushui11 ( Oryza sativa L. spp. japonica ) and treated by ethyl methane sulfonate (EMS). The plants were grown in a paddy field during natural seasons at the China National Rice Research Institute (Hangzhou, Zhejiang Province and Lingshui, Hainan Province, China). The chamber conditions of etiolated seedlings were as follows: the rice seeds were pre-soaked for 2 d and continued to germinate for 5 d in the dark. They were then transferred to light for 24 h. The mutant and wild-type (WT) Xushui11 in the heat treatment group were grown in an artificial climate chest under the growth condition of 42 °C for 16 h (day) and 35 °C for 8 h (night). The growing conditions were measured as previously described by Yang et al. ( 2016 2016 ). Map-based cloning of PGL3 and complementation assay 2 The pgl3 mutant was crossed with CH113 ( O. sativa L. spp. indica ) to generate an F 2 mapping population. One was mixed with 20 individuals with the pale-green leaf phenotype of pgl3 , and the other contained 20 individuals with a normal green phenotype of WT. A total of 181 simple sequence repeat (SSR) markers covering 12 chromosomes of rice were used for initial localization. For further mapping, 34 insertion-deletion (InDel) markers were designed using the Primer 5.0 software after comparison of the sequences between the japonica cultivar Nipponbare and the indica cultivar 9311. Sequences of primer pairs for mapping are listed in Table S1 S1 . Target DNA fragments in this region were amplified from pgl3 and WT plants, then sequenced and compared using DNAstar. A 6.2-kb genomic region, including 1.3-kb entire open reading frame (ORF) of PGL3 , a 4.1-kb upstream region, and a 0.8-kb downstream region (Lv et al., 2015 2015 ). The 6.2-kb fragment was cloned into the binary vector pCAMBIA1300 to generate the transformation construct, PGL3 -COM. The resulting construct was introduced into the embryogenic calli generated from the mature seed embryos of pgl3 using the Agrobacterium -mediated transformation method (Liu XQ et al., 2007 2007 ). RNA extraction and qRT-PCR 2 Total RNA was extracted from rice issues using a Total RNA Extraction kit (Axygen, cat No. AP-MN-MS-RNA-250, USA). The RNA was pre-treated with DNase I and used for cDNA synthesis using an RT-PCR kit (Promega, http://www.promega.com http://www.promega.com ). Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using a SYBR Premix Ex Taq kit (TaKaRa Bio Inc., China) in an Applied Biosystems ® 7500 Real-Time PCR System (Invitrogen, USA). Each analysis was repeated three times. The rice Actin 1 gene was used as a normalization control (Zhu et al., 2006 2006 ). Sequences of primer pairs for RT-PCR analysis are listed in Table S2 S2 . Transmission electron microscopy analysis 2 Leaves, 10 d after flowering, were fixed in a solution of 2.5% glutaraldehyde in phosphate buffer (pH 7.2) for 4 h at 4 °C. The polymerization and staining of the leaf samples were based on the method of Li et al. ( 2011 2011 ). The chloroplast ultrastructure of samples was observed by transmission electron microscope at Zhejiang University, Hangzhou, China. Chl content measurement and grain quality analysis 2 The total Chl from 0.4 g fresh leaves was extracted with 80% acetone in the dark at 28 °C for 24 h. The extract was analyzed using a spectrophotometer with light absorption values of 470, 645, and 663 nm (Wellburn, 1994 1994 ). The percentage of grains with chalkiness and the degree of endosperm chalkiness were measured as previously described by Zhou et al. ( 2015 2015 ). Three biological repeats were performed for each analysis. Detection of ROS 2 To monitor superoxide accumulation, leaves were excised and immediately placed in a 0.5 mg/ml nitroblue tetrazolium (NBT) solution in 10 mmol/L potassium phosphate buffer (pH 7.6) at 25 °C for 3 h in the dark. For hydrogen peroxide detection, excised leaves were treated with 1 mg/ml diaminobenzidine (DAB) in 50 mmol/L Tris acetate buffer (pH 5.0) at 25 °C for 24 h in the dark (Fukao et al., 2011 2011 ). The staining and bleaching of the samples was performed as previously described by Wang YH et al. ( 2013 2013 ). Results 1 Characterization of pgl3 2 The leaf color mutation was isolated from the mutagenesis of cultivar “Xiushui11” using EMS, which exhibited a pale-green leaf phenotype throughout the entire developmental process (Figs. 1a–1d 1a–1d ). The mutant was named pgl3 according to the phenotype. Leaves of the pgl3 mutant had a 68% and 70% reduction of Chl a and a 78% and 70% reduction of Chl b levels compared with those in WT at seedling and heading stages, respectively (Figs. 1e and 1f 1e and 1f ). Furthermore, the higher ratio of Chl a/Chl b may be due to the potential of Chl b synthesis in suffering a more severe decline than Chl a in the pgl3 mutant at seedling stage (data not shown). In addition, the Chl and carotene (Car) levels were dramatically decreased in pgl3 compared with WT. The pgl3 phenotypic characterizations indicated that PGL3 was essential for Chl synthesis. The low levels of Chl and Car may affect the growth and development of plant and further influence yield and quality of rice. As expected, pgl3 displayed shorter plant height, less tillers, and lower grain yield per plant than those of the WT (data not shown). The percentage of grains with chalkiness and the degree of endosperm chalkiness in pgl3 were higher than those in WT (Fig. S1 S1 ). Plants of WT (a) and pgl3 (b) at the seedling stage (bar, 2 cm) and plants of WT (c) and pgl3 (d) at the heading stage (bar, 10 cm). Contents of photosynthetic pigments at the seedling stage (e) and the heading stage (f). The values are presented as the mean±standard deviation (SD) from three biological replicates. ** Highly significance at P ≤0.01 vs. WT Map-based cloning of PGL3 2 To determine the genetic control of the pale-green leaf phenotype, we used a map-based cloning strategy to isolate the PGL3 gene. The pgl3 mutant was crossed with indica cultivar CH113 to generate a segregation population for gene mapping. The F 1 plants exhibited the normal green phenotype, suggesting that the mutant phenotype was controlled by a recessive gene(s). Among the F 2 individuals, 2313 were green leaf phenotype plants and 736 were pale-green leaf phenotype plants, fitting to a 3:1 ratio ( χ 2 =1.205 < =3.84). These results indicated that the mutation was controlled by a single recessive gene. The PGL3 locus was roughly mapped between markers RM22 and RM5474 on chromosome 3. The location of the PGL3 locus was ultimately limited to an 85-kb region between the markers A1 and A2, which included eleven ORFs (Fig. 2a 2a ). A sequencing analysis revealed that pgl3 had C-to-G and A-to-G nucleotide substitutions at positions 113 and 999 of the gene LOC_Os03g03990, resulting in Leu-to-Phe and Asp-to-Asn changes at 32nd and 328th amino acids, respectively (Fig. 2b 2b ). This gene encodes the chloroplast signal recognition particle 43 kDa protein (cpSRP43), which had been previously reported by Lv et al. ( 2015 2015 ) and Wang et al. ( 2016 2016 ). In the remainder of this paper, pgl3 will continue to use the mutant line name PGL3 as the gene name. For these reasons, this gene was identified as the candidate gene of pgl3 that caused the phenotype of pale-green leaves during the whole growth stage. (a) PGL3 locus was mapped to a region between markers RM22 and RM5474 on chromosome 3 (Chr.3). Fine-mapping of PGL3 to an 85-kb genomic DNA region between two makers Y1 and Y2. A bacterial artificial chromosome (BAC) contig (AC098693) cover the PGL3 locus. (b) Sixteen ORFs were predicted in the mapped region and sequence comparison revealed two substitutions of C to G and A to G in LOC_Os03g03990 in the pgl3 mutant. (c) Phenotype comparisons between WT and complemented plant (COM). (d) Chl a, Chl b, total Chl, and Car content of leaf in WT and complemented plant. The values are presented as the mean±SD from three biological replicates To further confirm the identity of the candidate gene as PGL3 , we performed a genetic complementation test by transforming the WT gene (LOC_Os03g03990) into the pgl3 mutant. All the positive transgenic lines completely rescued the pgl3 phenotypes, including leaf color and Chl and Car content (Figs. 2c and 2d 2c and 2d ). These results confirmed that PGL3 was indeed LOC_Os03g03990. PGL3 is required for Chl synthesis 2 We first compared PGL3 transcript levels in the pgl3 mutant and WT plants using RT-PCR. The transcript levels of PGL3 were always significantly decreased in pgl3 compared with that of WT (Fig. 3a 3a ). Next, we addressed the question of whether the pgl3 mutation affected the transcript of other genes associated with Chl synthesis. Compared with the WT, the expression of DVR (encoding divinyl reductase) was down-regulated and PORA (encoding NADPH-dependent protochlorophyllide oxidoreductase) was obviously down-regulated while the expression of others (glutamyl-tRNA reductase ( HEMA ), protochlorophyllide oxidoreductase B ( PORB ), and yellow green leaf 1 ( YGL1 )) was similar to that of the WT (data not shown). The changes in expression levels of these genes may lead to lower Chl content in pgl3 than in WT. (a) RT-PCR analysis of Chl synthesis-associated genes in WT and pgl3 . (b) Comparison of greening speed in WT and pgl3 etiolated seedlings that were exposed to light for 0–24 h. (c, d, e) Chl a, Chl b, and Chl content in the WT and pgl3 seedlings during greening. (f, g, h) RT-PCR analysis of Chl synthesis-associated genes in WT and pgl3 seedlings during greening: DVR , PORA , and PGL3 . The values are presented as the mean±SD from three biological replicates. * P ≤0.05, ** P ≤0.01 vs. WT To further clarify the effects of PGL3 on Chl synthesis, we subjected the WT and pgl3 etiolated seedlings to light for 24 h. The seedlings began to synthesize Chl after being exposed to light. Over 24 h of light growth, the WT had turned normal green early, and pgl3 was still albino (Fig. 3b 3b ). Comparing with that of its WT, the Chl synthesis rates of Chl a, Chl b, and Chl decreased remarkably in the mutant (Figs. 3c–3e 3c–3e ). At the same time, the expression of Chl synthesis-related genes was also detected in different periods. The expression levels of DVR and PGL3 were down-regulated and especially PGL3 was almost entirely unexpressed in pgl3 while the expression level of PORA decreased sharply after being exposed to light, revealing that it was very high in the dark and began to decrease in the light (Figs. 3f 3f ‒3h). This showed that PORA may have no effect on the greening process. These results demonstrated that PGL3 was necessary for Chl synthesis under light. pgl3 accelerates leaf senescence 2 ROS can cause extensive cell injury or death and become a marker of senescence (Davletova et al., 2005 2005 ). Superoxide anion (O 2 − ) and hydrogen peroxide (H 2 O 2 ) in the leaves of plants can be detected by NBT and DAB, respectively. In order to induce leaf senescence, the leaves of WT and pgl3 were cut and placed in the dark at 28 °C for 5 d. NBT and DAB staining of WT and mutant leaves showed that the leaf color of pgl3 was deeper than that of WT, indicating that the content of ROS in pgl3 leaves was higher than that in WT during the process of senescence (Figs. 4a–4c 4a–4c ). Next, transmission electron microscopic analysis was performed to determine if high ROS content in the pgl3 mutant affects the leaf senescence. The results revealed that the number and size of chloroplasts per cell in the mutant were very similar to those of the WT, whereas the grana stacks appeared less dense and a lot of plastoglobules were observed in the chloroplasts of pgl3 (Figs. 4d–4g 4d–4g ). These results indicated that the process of senescence was accelerated in the mutant. The transcription levels of ROS scavenging-related genes, OsAPX2 (encoding ascorbate peroxidase 2), OsCatB (encoding catalase), and OsPOD2 (encoding peroxidase precursor 2), were obviously down-regulated, while OsAPX1 (encoding ascorbate peroxidase 1) and OsPOD1 (encoding peroxidase precursor 1) showed no apparent change in pgl3 (Fig. 4h 4h ). The transcription level of senescence-inducible gene, OsAMTR1 (encoding aminotransferase), was obviously up-regulated in pgl3 . The changes in expression levels of these genes may be responsible for the leaf senescence exacerbated in pgl3 . (a) WT and pgl3 leaves were incubated in the dark for 0, 2, and 5 d. (b, c) WT and pgl3 leaves were stained with DAB (b) and NBT (c) during dark incubation. (d‒g) Ultrastructure of the chloroplast in the leaves of WT (d, e) and pgl3 (f, g). N, nucleus; C, chloroplast; P, plastoglobule. (h) RT-PCR analysis of senescence-associated genes in WT and pgl3 . The values are presented as the mean±SD from three biological replicates. * P ≤0.05, ** P ≤0.01 vs. WT pgl3 is sensitive to heat treatment 2 Continuous heat treatment will cause a reduction in cell-protective abilities and advance the aging process (Li et al., 2015 2015 ). We could see that WT and pgl3 exhibited a normal phenotype except for leaf color (Fig. 5a 5a ). pgl3 presented a severely wizened phenotype in the leaf tip under high temperatures for 5 d and the whole plant had become dry and withered under a high-temperature for 9 d, whereas there was no obvious signs of aging in the WT plants (Figs. 5b and 5c 5b and 5c ). The Chl a, Chl b, and Chl levels of pgl3 were rapidly decreased compared with WT during heat treatment (Figs. 5d–5f 5d–5f ). With the treatment of high temperatures, the electrolyte leakage was barely changed in WT, whereas it was increased sharply in pgl3 (Fig. 5g 5g ). These results indicate that PGL3 plays important roles in the process of heat tolerance. The plants of WT and pgl3 were grown under heat stress for 0 d (a), 5 d (b), and 9 d (c). The changes in Chl a (d), Chl b (e), and Chl (f) content under heat stress for 0, 1, 5, and 9 d. (g) Changes of electrolyte leakage under heat stress for 0, 1, 5, and 9 d. The values are presented as the mean±SD from three biological replicates 4 Discussion 2 In this study, the pgl3 plant mutant maintained a pale-green leaf color throughout its life cycle and had a sharp drop in Chl content compared with WT (Fig. 1 1 ). Those phenotypes were similar to the other leaf color mutants in rice although the pathways of those genes might be different. The leaf is the main organ for photosynthesis and the major source of carbohydrates. A Chl deficiency usually leads to the decline of supply nutrients for the rice grain at the filling stage, resulting in the reduction of grain yield and quality in pgl3 . AtcpSRP43 is a homolog of PGL3 and its mutant has similar phenotypes to pgl3 (Klimyuk et al., 1999 1999 ). By map-based cloning, the PGL3 gene was finally delimited in an 85-kb region on chromosome 3, including eleven ORFs (Fig. 2a 2a ). Sequence analysis showed that LOC_Os03g03990 was predicted to encode a cpSRP43 protein and to act as a chloroplast precursor, had two nucleotide substitutions in the coding region of pgl3 and led to the changes of the amino acid sequence coded (Fig. 2b 2b ). Furthermore, the leaf color and Chl content of pgl3 could be restored to the WT phenotype by transformation of the WT gene. Therefore, the gene LOC_Os03g03990 was indeed responsible for the pgl3 phenotype. Chl metabolism has been extensively studied in various organisms, and almost all of the Chl biosynthetic genes have been identified in higher plants (Nagata et al., 2005 2005 ). The Arabidopsis cpSRP43, together with cpSRP54, is involved in the pathway of light-harvesting chlorophyll-binding proteins (LHCPs) to the thylakoid (Groves et al., 2001 2001 ). The cpSRP pathway is necessary for the normal growth and development of a plant (Lv et al., 2015 2015 ). Therefore, the defect in OscpSRP43 seems to affect the transportation of LHCPs, leading to impaired Chl biosynthesis as well. Among the Chl synthesis-associated genes, we found that the expressions of three genes, including DVR , PORA , and PGL3 , were reduced at different levels at the seedling stage in pgl3 (Fig. 3a 3a ). The chlchlorophyll deficiency in pgl3 is likely due to the apparent down-regulation of DVR and PORA . PORA expression is repressed by light (Sakuraba et al., 2013 2013 ), which can explain why the expression level of PORA decreased sharply after being exposed to light, indicating that it was not essential for the process of greening. The cpSRP targets proteins in the chloroplast thylakoid membrane, and responds to high light intensity (Hutin et al., 2002 2002 ). Divinyl reductase converts 8-vinyl groups on various Chl intermediates to ethyl groups, which is indispensable for Chl biosynthesis (Wang et al., 2010 2010 ). The expression levels of PGL3 and DVR rapidly increased in WT plants after illumination, indicating that PGL3 and DVR may play a major role in Chl synthesis during greening in rice. ROS are thought to play a vital role in plant senescence (Mhamdi et al., 2010 2010 ). This is consistent with a loss in ROS scavenging capacity during the progression of senescence. So, the transcription levels of some ROS scavenging genes were detected, such as OsAPX1 , OsAPX2 , OsCatB , OsPOD1 , and OsPOD2 . For example, overexpression of OsAPX1 in rice prevents the over-accumulation of H 2 O 2 and reduces lipid peroxidation, thereby protecting plant development under stress (Sato et al., 2011 2011 ). The transcription levels of all ROS scavenging genes, which have a similar function, were obviously down-regulated except for OsPOD1 in pgl3 (Fig. 4h 4h ). Under normal conditions, redundant ROS were cleared by the ROS scavenging system in the plant. So pgl3 accumulated considerable ROS, which were detected by DAB and NBT during the aging process (Figs. 4b and 4c 4b and 4c ). The PGL3 may function as a regulator, which directly or indirectly regulates the level of ROS accumulation. Accumulation of unnecessary ROS induced the premature senescence of leaves and may even be involved in programmed cell death. This can explain why the characteristics of premature senescence exhibited early in pgl3 . Furthermore, OsAMTR1 is a specific expression gene of the aging process, which can be used as a molecular marker during the aging process (Liang et al., 2015 2015 ). OsAMTR1 , as a senescence-induced gene, was significantly higher in pgl3 compared with that in the WT. In addition, the number of plastoglobules also can be a sign of senescence, and it strongly increases during light stress, senescence, and in mutants blocked during thylakoid formation (Austin et al., 2006 2006 ; Bréhélin et al., 2007 2007 ). Although the WT produced some plastoglobules attached to thylakoid membranes, the number of plastoglobules in WT is far less than that in pgl3 at the heading stage (Fig. 4d 4d ). A rise in conjugated ROS led to leaf senescence in pgl3 . The process of senescence usually shows some important characteristics such as visible color changes, reduction in photosynthesis, and the degeneration of chloroplasts (Huang et al., 2016 2016 ). Leaf senescence will be accelerated when plants are exposed to environmental stresses, such as drought and heat conditions, which cause more ROS generation (Lee and Park, 2012 2012 ). During high temperature treatment, the plant of pgl3 grew extremely slowly and quickly turned withered and even died (Figs. 5a–5c 5a–5c ). Through the characterization of Chl content and electrolyte leakage, pgl3 demonstrated the characteristics of premature senescence (Figs. 5d–5g 5d–5g ). We may therefore infer that stable chloroplast structures can hold a steady level of electrolytes, and the aim of delaying leaf senescence has been achieved. In conclusion, we have conducted a study on a pale-green leaf mutant throughout the development of Xiushui11 rice. This mutation was controlled by a single nuclear gene PGL3 , which encodes for the cpSRP43 protein. PGL3 responded positively to the abiotic stress to delay senescence mainly by regulating the expression levels of ROS scavenging-associated genes. These results will not only provide a basis for further research on the PGL3 gene, but will also accelerate the explanation of the mechanism of OsSRP43 protein involved in Chl synthesis and ROS scavenging to rice senescence. References JR Austin , E Frost , PA Vidi , 等 . Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes . Plant Cell , 2006 . 18 ( 7 ): 1693 - 1703 . 16731586 DOI: 10.1105/tpc.105.039859 http://doi.org/10.1105/tpc.105.039859 . Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes C Bréhélin , F Kessler , KJ van Wijk . Plastoglobules: versatile lipoprotein particles in plastids . Trends Plant Sci , 2007 . 12 ( 6 ): 260 - 266 . 17499005 DOI: 10.1016/j.tplants.2007.04.003 http://doi.org/10.1016/j.tplants.2007.04.003 . Plastoglobules: versatile lipoprotein particles in plastids P Carol , D Stevenson , C Bisanz , 等 . Mutations in the Arabidopsis gene IMMUTANS cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation . Plant Cell , 1999 . 11 ( 1 ): 57 - 68 . 9878632 DOI: 10.1105/tpc.11.1.57 http://doi.org/10.1105/tpc.11.1.57 . Mutations in the Arabidopsis gene IMMUTANS cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation S Davletova , L Rizhsky , HJ Liang , 等 . Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis . Plant Cell , 2005 . 17 ( 1 ): 268 - 281 . 15608336 DOI: 10.1105/tpc.104.026971 http://doi.org/10.1105/tpc.104.026971 . Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis XJ Deng , HQ Zhang , Y Wang , 等 . Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid ( Oryza sativa L. ssp . indica) PLoS ONE , 2014 . 9 ( 6 ): e99564 DOI: 10.1371/journal.pone.0099564 http://doi.org/10.1371/journal.pone.0099564 . Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L. ssp T Fukao , E Yeung , J Bailey-Serres . The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice . Plant Cell , 2011 . 23 ( 1 ): 412 - 427 . 21239643 DOI: 10.1105/tpc.110.080325 http://doi.org/10.1105/tpc.110.080325 . The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice CH Goh , KH Jung , SK Roberts , 等 . Mitochondria provide the main source of cytosolic ATP for activation of outward-rectifying K + channels in mesophyll protoplast of chlorophyll-deficient mutant rice ( OsCHLH ) seedlings . J Biol Chem , 2004 . 279 ( 8 ): 6874 - 6882 . 14660680 DOI: 10.1074/jbc.M309071200 http://doi.org/10.1074/jbc.M309071200 . Mitochondria provide the main source of cytosolic ATP for activation of outward-rectifying K+ channels in mesophyll protoplast of chlorophyll-deficient mutant rice (OsCHLH) seedlings MR Groves , A Mant , A Kuhn , 等 . Functional characterization of recombinant chloroplast signal recognition particle . J Biol Chem , 2001 . 276 ( 30 ): 27778 - 27786 . 11356852 DOI: 10.1074/jbc.M103470200 http://doi.org/10.1074/jbc.M103470200 . Functional characterization of recombinant chloroplast signal recognition particle JL Huang , F Qin , GC Zang , 等 . Mutation of OsDET1 increases chlorophyll content in rice . Plant Sci , 2013 . 210 241 - 249 . 23849131 DOI: 10.1016/j.plantsci.2013.06.003 http://doi.org/10.1016/j.plantsci.2013.06.003 . Mutation of OsDET1 increases chlorophyll content in rice QN Huang , YF Shi , XB Zhang , 等 . Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice . J Integr Plant Biol , 2016 . 58 ( 1 ): 12 - 28 . 26040493 DOI: 10.1111/jipb.12372 http://doi.org/10.1111/jipb.12372 . Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice C Hutin , M Havaux , JP Carde , 等 . Double mutation cpSRP43 – /cpSRP54 – is necessary to abolish the cpSRP pathway required for thylakoid targeting of the light-harvesting chlorophyll proteins . Plant J , 2002 . 29 ( 5 ): 531 - 543 . 11874567 DOI: 10.1046/j.0960-7412.2001.01211.x http://doi.org/10.1046/j.0960-7412.2001.01211.x . Double mutation cpSRP43–/cpSRP54– is necessary to abolish the cpSRP pathway required for thylakoid targeting of the light-harvesting chlorophyll proteins VI Klimyuk , F Persello-Cartieaux , M Havaux , 等 . A chromodomain protein encoded by the Arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting . Plant Cell , 1999 . 11 ( 1 ): 87 - 99 . 9878634 DOI: 10.1105/tpc.11.1.87 http://doi.org/10.1105/tpc.11.1.87 . A chromodomain protein encoded by the Arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting M Kusaba , H Ito , R Morita , 等 . Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence . Plant Cell , 2007 . 19 ( 4 ): 1362 - 1375 . 17416733 DOI: 10.1105/tpc.106.042911 http://doi.org/10.1105/tpc.106.042911 . Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence S Lee , C Park . Regulation of reactive oxygen species generation under drought conditions in Arabidopsis . Plant Signal Behav , 2012 . 7 ( 6 ): 599 - 601 . 22580707 DOI: 10.4161/psb.19940 http://doi.org/10.4161/psb.19940 . Regulation of reactive oxygen species generation under drought conditions in Arabidopsis S Lee , JH Kim , ES Yoo , 等 . Differential regulation of chlorophyll a oxygenase genes in rice . Plant Mol Biol , 2005 . 57 ( 6 ): 805 - 818 . 15952067 DOI: 10.1007/s11103-005-2066-9 http://doi.org/10.1007/s11103-005-2066-9 . Differential regulation of chlorophyll a oxygenase genes in rice W Li , SH Zhong , GJ Li , 等 . Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence . Cell Res , 2011 . 21 ( 5 ): 835 - 848 . 21221134 DOI: 10.1038/cr.2011.4 http://doi.org/10.1038/cr.2011.4 . Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence XM Li , DY Chao , Y Wu , 等 . Natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of African rice . Nat Genet , 2015 . 47 ( 7 ): 827 - 833 . 25985140 DOI: 10.1038/ng.3305 http://doi.org/10.1038/ng.3305 . Natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of African rice CZ Liang , GY Zheng , WZ Li , 等 . Melatonin delays leaf senescence and enhances salt stress tolerance in rice . J Pineal Res , 2015 . 59 ( 1 ): 91 - 101 . 25912474 DOI: 10.1111/jpi.12243 http://doi.org/10.1111/jpi.12243 . Melatonin delays leaf senescence and enhances salt stress tolerance in rice WZ Liu , YP Fu , GC Hu , 等 . Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice ( Oryza sativa L.) . Planta , 2007 . 226 ( 3 ): 785 - 795 . 17541632 DOI: 10.1007/s00425-007-0525-z http://doi.org/10.1007/s00425-007-0525-z . Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.) XQ Liu , XQ Bai , XJ Wang , 等 . OsWRKY71, a rice transcription factor, is involved in rice defense response . J Plant Physiol , 2007 . 164 ( 8 ): 969 - 979 . 16919842 DOI: 10.1016/j.jplph.2006.07.006 http://doi.org/10.1016/j.jplph.2006.07.006 . OsWRKY71, a rice transcription factor, is involved in rice defense response SP Long , XG Zhu , SL Naidu , 等 . Can improvement in photosynthesis increase crop yields? . Plant Cell Environ , 2006 . 29 ( 3 ): 315 - 330 . 17080588 DOI: 10.1111/j.1365-3040.2005.01493.x http://doi.org/10.1111/j.1365-3040.2005.01493.x . Can improvement in photosynthesis increase crop yields? XG Lv , YF Shi , X Xu , 等 . Oryza sativa chloroplast signal recognition particle 43 (OscpSRP43) is required for chloroplast development and photosynthesis . PLoS ONE , 2015 . 10 ( 11 ): e0143249 26600124 DOI: 10.1371/journal.pone.0143249 http://doi.org/10.1371/journal.pone.0143249 . Oryza sativa chloroplast signal recognition particle 43 (OscpSRP43) is required for chloroplast development and photosynthesis A Mhamdi , G Queval , S Chaouch , 等 . Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models . J Exp Bot , 2010 . 61 ( 15 ): 4197 - 4220 . 20876333 DOI: 10.1093/jxb/erq282 http://doi.org/10.1093/jxb/erq282 . Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models R Mittler , S Vanderauwera , M Gollery , 等 . Reactive oxygen gene network of plants . Trends Plant Sci , 2004 . 9 ( 10 ): 490 - 498 . 15465684 DOI: 10.1016/j.tplants.2004.08.009 http://doi.org/10.1016/j.tplants.2004.08.009 . Reactive oxygen gene network of plants N Nagata , R Tanaka , S Satoh , 等 . Identification of a vinyl reductase gene for chlorophyll synthesis in Arabidopsis thaliana and implications for the evolution of Prochlorococcus species . Plant Cell , 2005 . 17 ( 1 ): 233 - 240 . 15632054 DOI: 10.1105/tpc.104.027276 http://doi.org/10.1105/tpc.104.027276 . Identification of a vinyl reductase gene for chlorophyll synthesis in Arabidopsis thaliana and implications for the evolution of Prochlorococcus species SY Park , JW Yu , JS Park , 等 . The senescence-induced staygreen protein regulates chlorophyll degradation . Plant Cell , 2007 . 19 ( 5 ): 1649 - 1664 . 17513504 DOI: 10.1105/tpc.106.044891 http://doi.org/10.1105/tpc.106.044891 . The senescence-induced staygreen protein regulates chlorophyll degradation L Piquery , C Davoine , C Huault , 等 . Senescence of leaf sheaths of ryegrass stubble: changes in enzyme activities related to H 2 O 2 metabolism . Plant Growth Regul , 2000 . 30 ( 1 ): 71 - 77 . DOI: 10.1023/A:1006308928018 http://doi.org/10.1023/A:1006308928018 . Senescence of leaf sheaths of ryegrass stubble: changes in enzyme activities related to H2O2 metabolism S Ramundo , D Casero , T Mühlhaus , 等 . Conditional depletion of the Chlamydomonas chloroplast ClpP protease activates nuclear genes involved in autophagy and plastid protein quality control . Plant Cell , 2014 . 26 ( 5 ): 2201 - 2222 . 24879428 DOI: 10.1105/tpc.114.124842 http://doi.org/10.1105/tpc.114.124842 . Conditional depletion of the Chlamydomonas chloroplast ClpP protease activates nuclear genes involved in autophagy and plastid protein quality control RA Richards . Selectable traits to increase crop photosynthesis and yield of grain crops . J Exp Bot , 2000 . 51 ( Suppl 1 ): 447 - 458 . 10938853 DOI: 10.1093/jexbot/51.suppl_1.447 http://doi.org/10.1093/jexbot/51.suppl_1.447 . Selectable traits to increase crop photosynthesis and yield of grain crops Y Sakuraba , ML Rahman , SH Cho , 等 . The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions . Plant J , 2013 . 74 ( 1 ): 122 - 133 . 23289852 DOI: 10.1111/tpj.12110 http://doi.org/10.1111/tpj.12110 . The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions Y Sato , R Morita , S Katsuma , 等 . Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice . Plant J , 2009 . 57 ( 1 ): 120 - 131 . 18778405 DOI: 10.1111/j.1365-313X.2008.03670.x http://doi.org/10.1111/j.1365-313X.2008.03670.x . Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice Y Sato , Y Masuta , K Saito , 等 . Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa . Plant Cell Rep , 2011 . 30 ( 3 ): 399 - 406 . DOI: 10.1007/s00299-010-0985-7 http://doi.org/10.1007/s00299-010-0985-7 . Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa P Wang , J Gao , C Wan , 等 . Divinyl chlorophyll (ide) a can be converted to monovinyl chlorophyll (ide) a by a divinyl reductase in rice . Plant Physiol , 2010 . 153 ( 3 ): 994 - 1003 . 20484022 DOI: 10.1104/pp.110.158477 http://doi.org/10.1104/pp.110.158477 . Divinyl chlorophyll (ide) a can be converted to monovinyl chlorophyll (ide) a by a divinyl reductase in rice P Wang , C Wan , Z Xu , 等 . One divinyl reductase reduces the 8-vinyl groups in various intermediates of chlorophyll biosynthesis in a given higher plant species, but the isozyme differs between species . Plant Physiol , 2013 . 161 ( 1 ): 521 - 534 . 23154534 DOI: 10.1104/pp.112.208421 http://doi.org/10.1104/pp.112.208421 . One divinyl reductase reduces the 8-vinyl groups in various intermediates of chlorophyll biosynthesis in a given higher plant species, but the isozyme differs between species YH Wang , LR Zhang , LL Zhang , 等 . A novel stress-associated protein SbSAP14 from Sorghum bicolor confers tolerance to salt stress in transgenic rice . Mol Breed , 2013 . 32 ( 2 ): 437 - 449 . DOI: 10.1007/s11032-013-9882-4 http://doi.org/10.1007/s11032-013-9882-4 . A novel stress-associated protein SbSAP14 from Sorghum bicolor confers tolerance to salt stress in transgenic rice ZW Wang , TQ Zhang , YD Xing , 等 . YGL9 , encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development . J Integr Agric , 2016 . 15 ( 5 ): 944 - 953 . DOI: 10.1016/s2095-3119(15)61310-7 http://doi.org/10.1016/s2095-3119(15)61310-7 . YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development AR Wellburn . The spectral determination of chlorophylls a and b , as well as total carotenoids, using various solvents with spectrophotometers of different resolution . J Plant Physiol , 1994 . 144 ( 3 ): 307 - 313 . DOI: 10.1016/S0176-1617(11)81192-2 http://doi.org/10.1016/S0176-1617(11)81192-2 . The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution XY Wu , BK Kuai , JZ Jia , 等 . Regulation of leaf senescence and crop genetic improvement . J Integr Plant Biol , 2012 . 54 ( 12 ): 936 - 952 . 23131150 DOI: 10.1111/jipb.12005 http://doi.org/10.1111/jipb.12005 . Regulation of leaf senescence and crop genetic improvement YL Yang , J Xu , LC Huang , 等 . PGL , encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice . J Exp Bot , 2016 . 67 ( 5 ): 1297 - 1310 . 26709310 DOI: 10.1093/jxb/erv529 http://doi.org/10.1093/jxb/erv529 . PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice F Zhang , X Luo , B Hu , 等 . YGL138 ( t ), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice . Rice , 2013 . 6 ( 1 ): 7 24280537 DOI: 10.1186/1939-8433-6-7 http://doi.org/10.1186/1939-8433-6-7 . YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice H Zhang , J Li , JH Yoo , 等 . Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development . Plant Mol Biol , 2006 . 62 ( 3 ): 325 - 337 . 16915519 DOI: 10.1007/s11103-006-9024-z http://doi.org/10.1007/s11103-006-9024-z . Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development L Zhou , S Liang , K Ponce , 等 . Factors affecting head rice yield and chalkiness in indica rice . Field Crops Res , 2015 . 172 1 - 10 . DOI: 10.1016/j.fcr.2014.12.004 http://doi.org/10.1016/j.fcr.2014.12.004 . Factors affecting head rice yield and chalkiness in indica rice YY Zhu , T Nomura , YH Xu , 等 . ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice . Plant Cell , 2006 . 18 ( 2 ): 442 - 456 . 16399803 DOI: 10.1105/tpc.105.038455 http://doi.org/10.1105/tpc.105.038455 . ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice Compliance with ethics guidelines Jing YE, Yao-long YANG, Xing-hua WEI, Xiao-jun NIU, Shan WANG, Qun XU, Xiao-ping YUAN, Han-yong YU, Yi-ping WANG, Yue FENG, and Shu WANG declare that they do not have any conflict of interest. This article does not contain any studies with human or animal subjects performed by any of the authors. 10.1631/jzus.B1700337 Project supported by the National Natural Science Foundation of China (No. 31521064), the Chinese Academy of Agricultural Sciences (No. CAAS-ASTIP-201X-CNRRI), and the Zhejiang Provincial Natural Science Foundation of China (Nos. LQ15C130001 and LY16C060003) 2017-07-03 2017-10-18 2022-01-05 Copyright © Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2018 2018 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 04 19