Evaluation of organ distribution of microcystins in the freshwater phytoplanktivorous fish Hypophthalmichthys molitrix

Evaluation of organ distribution of microcystins in the freshwater phytoplanktivorous fish Hypophthalmichthys molitrix first-author corresp ‡Corresponding Author ‡Corresponding Author †E-mail:zjy@zju.edu.cn †E-mail:zjy@zju.edu.cn zjy@zju.edu.cn School of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China School of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China To evaluate the public health risk of exposure to microcystins in fish food in China, the distribution pattern of microcystin-LR and microcystin-RR in various organs (liver, intestine, kidney, muscle and lipid) of the dominant freshwater phytoplanktivorous fish Hypophthalmichthys molitrix in Hangzhou, China’s Tiesha River was investigated with the method of HPLC-ESI-MS analysis. The distribution of microcystins was different in the fish organs and the major total microcystins (microcystin-LR and microcystin-RR) were present in the intestines (6.49 μg/g fresh weight), followed by the livers (4.52 μg/g fresh weight) and the muscles (2.86 μg/g fresh weight). Microcystins were detected in kidneys (1.35 μg/g fresh weight), but not detected in lipid. The results suggested that the mean daily intake from fish was 0.03 μg/kg body weight which was very close to the recommended WHO tolerable daily intake (TDI) level of 0.04 μg/kg body weight per day, and local people were warned they may have health risk if they consumed fish from the river. Microcystin Organ distribution Bioaccumulation Fish Risk INTRODUCTION 1 Cyanobacterial blooms are becoming an important water quality problem all over the world including China. Microcystins are the most widespread type of cyanobacterial toxins in freshwater systems, which represent a potential stress and hazard to aquatic animal population (Huynh-Delerme et al., 2005 2005 ). Bioaccumulation of microcystins by fish has been reported by several authors (Tencalla et al., 1994 1994 ; de Magalhães et al., 2001 2001 ) and microcystins are also believed to be involved in the large-scale die-off of fish (Ibelings et al., 2005 2005 ). Because fish is at the most important trophic level in the aquatic food chain, microcystins may pose a common health risk to human through a food-chain transfer process in the case of fish as an important human daily food source. In 1998, heavy cyanobacterial blooms were observed in the Tiesha River, with Microcystis aeruginosa being a predominant species in the bloom material (Wang et al., 2003 2003 ). It was reported that this bloom was toxic, with colorimetric protein phosphatases inhibition assay revealing that microcystin levels in surface water were higher than the WHO maximum allowable concentration for MCYST (microcystin) in drinking water of 1 μg/L (Falconer et al., 1994 1994 ). Although some measures such as using the phytoplanktivorous freshwater fish to digest cyanobacteria have been applied to control these blooms in the river, recently we found trace level of microcystin-LR and microcystin-RR in the river. H . molitrix has been the dominant species in the Tiesha River and is often harvested by local fishermen for food. As phytoplanktivorous fish, H . molitrix are especially important to human because of its role in aquatic ecosystems as direct consumers of phytoplankton and potential for biological management of algal blooms. They may digest much more toxic cyanobacteria in normal diets compared with the herbivorous and carnivorous fish. This study was aimed at examining the distribution patterns of microcystins in H . molitrix and at evaluating the effects of bioaccumulated microcystins in the organs and the risks for fish food chain in the Tiesha River. MATERIALS AND METHODS 1 Chemicals 2 Microcystin-LR (CAS No. 101043-37-2, C 49 H 74 N 10 O 12 , molecular weight: 995.2, purity > 95%) and microcystin-RR (CAS No. 111755-37-4, C 49 H 75 N 13 O 12 , molecular weight: 1038.2, purity > 95%) were purchased from Sigma (St. Louis, MO, USA). Methanol and acetonitrile were HPLC grade, and all other reagents were analytical grade. Standard solutions were prepared in methanol and stored at −20 °C. The Oasis HLB (hydrophilic-lipophilic balance) solid phase extraction cartridges were purchased from Waters Corporation (Milford, MA, USA). Cleaning-up method for fish samples 2 The analyzed fish ( H . molitrix ) harvested by local fishermen in the Tiesha River were dissected into five parts: intestine, liver, muscle, kidney and lipid, then immediately frozen at −20 °C by liquid nitrogen in the laboratory. The purification of microcystins for analysis was based on de Magalhães et al.( 2001 2001 ) with some modification. Each fresh tissue sample (0.5 g) was extracted twice by 75% methanol (5 ml) with a supersonic instrument for 30 min. The methanol extract was centrifuged at 12 000 g/min for 10 min. Then the extract was mixed three times with 2 ml hexane. Hexane layers were discarded and the methanol extract was diluted with 10 ml water. The extract was concentrated and cleaned using an Oasis HLB solid phase extraction cartridge. The cartridges were washed with 2 ml of water after the adsorption of the targeted compounds. The cartridges were eluted with 1 ml of 90% methanol with 0.1% trifluoroacetic acid (TFA). The elution was evaporated dry, and the residue was dissolved in 0.2 ml of water. The final water solution was subjected to HPLC-ESI-MS analysis. HPLC-ESI-MS analysis 2 An agilent 1100 HPLC system (Palo Alto, CA, USA) equipped with degasser, autosampler, a binary pump, and a mass spectrometer was used to analyze specific microcystins. The separation was performed using a 5 μm Zorbax SB-C18 column (150 mm×2.0 mm i.d.). The column temperature was set at 30 °C, controlled by temperature controller TC100 and HT-130 column heater. The mobile phase consisted of acetonitrile and water (0.5% formic acid) at a ratio of 35/65 (v/v). The flow rate was set at 0.3 ml/min. Sample injection volumes were typically 20 μl. The mass spectrometer was a Quattro Micro (Micromass, Manchester, UK) triple quadrupole MS with electrospray ionization (ESI). Samples were introduced directly to the mass spectrometer via the HPLC column. The capillary voltage was set at 4.0 kV and the fragment voltage at 100 V. The desolvation gas (nitrogen) temperature and flow-rate were set at 350 °C and 11 L/min, respectively. The ion source temperature was set at 120 °C. The instrument was operated in the positive ion mode. In full-scan mode, mass spectra of 500 to 1200 m / z were obtained. In the selected-ion monitoring (SIM) mode, two ions, 519.9 (microcystin-RR, [M+2H] 2+ ) and 995.6 (microcystin-LR, [M+H] + ) were monitored. During the HPLC-ESI-MS analysis we obtained a calculated limit of detection (LOD) of 0.02 μg/g fresh tissue. Recovery percentages were evaluated from spiked samples with recoveries of 74.2% for microcystin-LR and 88.4% for microcystin-RR, respectively. RESULTS 1 Standards and samples mass spectrum 2 Depending on the amino acids in the microcystin structure, mono [M+H] + or double charged [M+2H] 2+ ions were obtained. The mass spectra of microcystin-LR showed mainly the single charged ions [M+H] + at 995.6 (Fig. 1a 1a ). But the main mass peak observed for microcystin-RR was that of the double charged ion [M+2H] 2+ at 519.9 m / z , probably due to the presence of two arginine residues (Fig. 1b 1b ). All ESI+mass spectra obtained were similar to those reported in the literature (Barco et al., 2002 2002 ). The chromatograms of the intestine of H . molitrix were measured in full scan mode (Fig. 2 2 ) and SIM mode (Fig. 3 3 ). We found that the SIM mode was over 8 times that of the full scan extraction ion mode for both two microcystins in HPLC-ESI-MS determination. Detected microcystins in different organs 2 Fig. 4 4 presents the distribution of microcystins in the organs of the fish H . molitrix . The highest level of microcystin-LR was found in the intestines, followed by the livers and kidneys. Small amounts of microcystin-LR were detected in the muscles. But microcystin-LR and microcystin-RR was not detected in the lipid. Microcystin-RR apparently showed the same distribution pattern as that of microcystin-LR. Furthermore, we found the concentration of microcystin-RR was about 2 times that of microcystin-LR. DISCUSSION 1 Bioaccumulation of microcystins in fish has been well documented (Malbrouck et al., 2003 2003 ; Mohamed et al., 2003 2003 ). Microcystins were often detected in livers, muscles and other tissues. Phytoplanktivorous, carnivorous, omnivorous and herbivorous fish have different microcystins patterns. In a sub-chronic toxicity experiment, Xie et al.( 2004 2004 ) found that microcystin-RR was detectable in the liver, muscle and blood samples of the phytoplanktivorous silver carp while no microcystin-LR was detectable in the muscle and blood samples in spite of the abundant presence of microcystin-LR in the intestines. Xie et al.( 2005 2005 ) found that no microcystins were detected in the intestines of the herbivorous Parabramis pekinensis , the carnivorous Culter illshaeformis and the Pseudobagrus fulvidraco , but in the phytoplanktivorous H . molitrix the highest amount of microcystins was detected in the intestines. In this study, the application of our proposed method showed the transfer of microcystins to different fish tissues (Fig. 4 4 ). We found the maximal total microcystins (microcystin-LR and microcystin-RR) were present in the intestines, followed by the livers and the muscles, but microcystins detected in kidneys were minimal. Dawson ( 1998 1998 ) found microcystins are always bioaccumulated by the fish liver according to the bile transfer pathway. But in this study, the highest levels of microcystins were in intestines. We found a large number of Microcystis aeruginosa in the dissected fish samples intestines, within microcystins concentration was relatively high. Microcystins were included in the final analysis. Specziár et al.( 1997 1997 ) reported that cyanobacteria Microcystis aeruginosa was more abundant in phytoplanktivorous than omnivorous fish, since omnivorous fish normally consume zooplankton and detritus. So our finding also confirmed that H. molitrix could graze high numbers of toxic Microcystis cells. de Maagd et al.( 1999 1999 ) found that log( n -octanol: water distribution ratio) [log(DOW)] of microcystin-LR was low, ranging from −1.76 to 2.18. These results suggested that microcystins could scarcely be accumulated through the epithelia of aquatic organisms. During this field study, there were no microcystins detected in the lipid tissues which further confirmed that the very low hydrophobicity and polar functions of microcystin-LR in neutral condition may inhibit their distribution into lipid tissues (Rivasseau et al., 1998 1998 ). We also found microcystins in the internal tissues (liver, kidney, muscle and intestine) of H . molitrix , so ingestion can be proposed as the most probable route of toxin uptake in this wild fish. For human health risk, two oral routes can lead to microcystins exposure: direct ingestion of water containing microcystins and the consumption of aquatic animals which have ingested cyanobacteria and accumulated microcystins (de Magalhães et al., 2001 2001 ). Thus, transfer of microcystins to humans through consumption of contaminated fish is a potential threat to human health. The WHO has determined a tolerable daily intake (TDI) value of 0.04 μg/kg body weight per day for microcystin-LR (Chorus and Bartram, 1999 1999 ). TDI is defined as the acceptable amount of a potentially toxic substance that can be consumed daily during a life period. According to Gupta et al.( 2003 2003 ), the LD 50 in mice for microcystin-RR is about 5 times that for microcystin-LR, so coefficient of 0.2 was used to convert microcystin-RR into microcystin-LR equivalent. Based on our results, for a 60 kg adult, who ingests on the average 100 g of fish a day, the concentration of microcystin-LR eq in fish samples reaches 1.8 μg/g fresh weight, representing an estimated daily intake of 0.03 μg/kg body weight. This is very close to the TDI value suggested by WHO. Microcystins are chemically heat stable (Harada et al., 1996 1996 ), and their structures are not broken down by cooking. So consuming toxic fishes in the Tiesha River is clearly a health risk to the local public. Therefore, the long-term impact of mirocystins to the local public health needs to be further investigated in the future. References M Barco , J Rivera , J Caixach . 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Organ distribution and bioaccumulation of microcystins in freshwater fish at different trophic levels from the eutrophic lake Chaohu, China . Environ Toxicol , 2005 . 20 ( 3 ): 293 - 300 . 15892067 DOI: 10.1002/tox.20120 http://doi.org/10.1002/tox.20120 . Organ distribution and bioaccumulation of microcystins in freshwater fish at different trophic levels from the eutrophic lake Chaohu, China The authors would like to thank Ph.D candidate Xiaodan Wu for having supported part of this work. 10.1631/jzus.2007.B0116 Project (No. Y505257) supported by the Natural Science Foundation of Zhejiang Province, China 2006-01-16 2006-05-01 2022-01-05 Copyright © 2007, Journal of Zhejiang University Science 2007 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 02 08