Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal

Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal 1 2 first-author †E-mail:zhang-wj1022@tom.comzrxu@zju.edu.cn †E-mail:zhang-wj1022@tom.comzrxu@zju.edu.cn zhang-wj1022@tom.com 1 corresp ‡Corresponding Author ‡Corresponding Author †E-mail:zhang-wj1022@tom.comzrxu@zju.edu.cn †E-mail:zhang-wj1022@tom.comzrxu@zju.edu.cn zhang-wj1022@tom.com 1 1 Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310029, China Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310029, China College of Animal Science and Technology, Shihezi University, Shihezi 832003, China College of Animal Science and Technology, Shihezi University, Shihezi 832003, China The objective of this work was to investigate the effect of six individual strains of fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal (CSM). Six groups of disinfected CSM substrate were incubated for 48 h after inoculation with either of the fungi C . capsuligena ZD-1, C . tropicalis ZD-3, S . cerevisae ZD-5, A . terricola ZD-6, A . oryzae ZD-7, or A . niger ZD-8. One not inoculated group (substrate) was used as a control. Levels of initial and final free gossypol (FG), crude protein (CP), amino acids (AA) and in vitro digestibility were assayed. The experiment was done in triplicate. The experimental results indicated that microbial fermentation could greatly decrease ( P < 0.05) FG levels in CSM. The detoxification efficiency differed between the species of microorganisms applied. From the perspective of reducing CSM potential toxicity, C . tropicalis ZD-3 was most successful followed by S . cerevisae ZD-5 and A . niger ZD-8. They could reduce FG levels of CSM to 29.8, 63.07 and 81.50 mg/kg based on DM (dry matter), respectively, and their detoxification rates were 94.57%, 88.51% and 85.16%, respectively. If crude protein, amino acids content and their in vitro digestibility were also taken into account, A . niger ZD-8 may be the best choice. The CP content of CSM substrate fermented by C . tropicalis ZD-3 and A . niger ZD-8 were improved by 10.76% and 22.24%; the TAA (total amino acids) contents were increased by 7.06% and 11.46%, and the EAA (essential amino acids) were raised by 7.77% and 12.64%, respectively. Especially, the levels of methionine, lysine and threonine were improved greatly ( P < 0.05). The in vitro CP digestibility of CSM fermented by C . tropicalis ZD-3 and A. niger ZD-8 was improved by 13.42% and 18.22%, the TAA were increased by 17.75% and 22.88%, and the EAA by 16.61% and 21.01%, respectively. In addition, the in vitro digestibility of methionine, lysine and threonine was also improved greatly ( P < 0.05). Fungi Free gossypol Solid substrate fermentation Cottonseed meal Detoxification Nutritional value INTRODUCTION 1 The potential of cottonseed meal (CSM) protein for animal nutrition is limited by the presence of gossypol (C 30 H 30 O 8 ), a toxic polyphenolic pigment (Francis et al., 2001 2001 ). It is produced in the seeds of the cotton plant, and feeding diets containing gossypol cause negative effects such as growth depression and intestinal and other internal organ abnormalities (Berardi and Goldblatt, 1980 1980 ; Robinson et al., 2001 2001 ). Its negative effect on animal health has long been recognized, and the toxic effect of gossypol is much greater for non-ruminants than ruminants due to binding of free gossypol (FG) to soluble proteins in the rumen (Willard et al., 1995 1995 ). Thus, if FG was transformed into bound gossypol (BG), it would not harm animals, because BG cannot be absorbed through the digestive tract. Cottonseeds are commonly processed into oil and meal, which may contain high concentrations of the toxin, and further processing is necessary to reduce it to permissible levels. The development of glandless cottonseed by plant breeders to overcome this problem was limited due to the poorer yields and the increased susceptibility of this crop to insects and diseases. Consequently glandless cottonseed accounts for less than 0.5% of the total crop in the world. A number of methods have been developed for removing gossypol from cottonseed including solvent extraction of free gossypol (Damaty and Hudson, 1975 1975 ; Canella and Sodini, 1977 1977 ; Cherry and Gray, 1981 1981 ; Rahma and Narasingo Rao, 1984 1984 ), ferrous sulfate treatment (Tabatabai et al., 2002 2002 ; Barraza et al., 1991 1991 ), calcium hydroxide treatment (Nagalakshmi et al., 2002 2002 ; 2003 2003 ), microbial fermentation (Wu and Chen, 1989 1989 ; Shi et al., 1998 1998 ) and so on. These methods play an important role in detoxification of CSM, but the reduction of gossypol using solvent system suffers from the difficulty of totally removing residual solvents which have potentially harmful effects, as adversely affect the flavor. Ferrous sulfate treatment causes feed to become black which affects feed quality, and calcium hydroxide treatment often leads to the problem of reduced vitamin content. Microbial fermentation should be a kind of promising detoxification method, but literature on CSM fermentation is scarce, and the microbial species for investigation are limited. The objective of this work was to study the effect of selected fungi on the reduction of gossypol levels during solid substrate fermentation of CSM. Several representative fungal strains are bred by Zhejiang University, China. We examined whether the selected fungi through fermentation leads to reduction of the gossypol levels; which strain is more effective; and whether the amino acids (AA) profile and their in vitro digestibility are changed, especially essential amino acids (EAA) such as lysine and methionine. MATERIALS AND METHODS 1 Substrate treatment 2 CSM was obtained from Shihezi district, Xinjiang autonomous region, China. This material was mixed with corn flour and wheat bran, the ratio of CSM:corn flour:wheat bran was 7:2:1. Then the mixture was moistened and the ratio of mixture versus water was 1:0.8. Afterwards, it was autoclaved at 112.6 °C for 20 min. Microorganisms and inoculum 2 The strains Candida capsuligena ZD-1, Candida tropicalis ZD-3, Saccharomyces cerevisae ZD-5, Aspergillus terricola ZD-6, Aspergillus oryzae ZD-7, and Aspergillus niger ZD-8 were used in this work. They were all bred and collected by the Feed Science Institute, Zhejiang University. Among them, C . capsuligena ZD-1 and A . oryzae ZD-7 (used as reference research) could not be grown on Czapek’s medium containing 0.5% of gossypol instead of sucrose as carbon source, but the other four strains grew well. The four strains were bred by a screening method of increasing gossypol concentration in Czapek’s medium without sugar step by step, ultraviolet irradiation, chemical mutagen treatment and so on, at last rejuvenation by malt extract medium. Stock cultures were maintained on malt extract agar slants. Yeasts inocula were grown in 50 ml malt extract (5 °Bè) in 150 ml conical flasks at 30 °C for 48 h at 200 r/min. Filamentous fungal spores were washed from a 7-day agar slant culture with 10 ml sterile distilled water, and 5 ml aliquots were added to 100 g of sterilized solid substrate consisting of soybean meal and wheat bran (6:4, w/w), with adjusted moisture of 50% in 500 ml conical flasks, and incubated at 30 °C for 3 d in a 95% relative humidity chamber, then oven dried at 45 °C for 24 h, and processed into flour for the experimental inoculation. Solid substrate fermentation 2 The treated substrate 100 g in each 500 ml conical flask was then inoculated with 5 ml either of yeast inocula, or 1% (w/w) mycelial inocula of filamentous fungi, and incubated at 30 °C for 48 h in a 95% relative humidity chamber. Triplicate flasks were set up for each experimental variation. Sample processing 2 After fermentation was completed, every flask of fermented substrate was dried in an oven at 60 °C for 48 h respectively, and dry weight loss was determined. Then they were processed into flour for related index determination. Related index assay 2 The dry matter (DM) content was measured by dying at 105 °C for 5 h. The crude protein (CP) assay was by Kjeldahl method (AOAC, 1999 1999 ). FG was determined by the official method of the American Oil Chemists Society (AOCS, 1989 1989 ). Amino acids assay was performed by the Center of Analysis and Measurements of Zhejiang University according to the AOAC ( 1984 1984 ) method, using Beckman 6300 (Beckman Instrument, USA). In vitro digestibility determination 2 Analysis procedure used was after the method of in vitro digestibility determination published by Sakamoto et al.( 1980 1980 ) and with little changes. Fermented or non-fermented CSM (10 g, exactly weighed) were put into 250 ml conical flasks. Then 30 ml 0.1 mol/L HCl and 30 mg pepsin were added and blended evenly and incubated at 39 °C at 150 r/min for 4 h. Then pH was adjusted to 7.0 and 30 ml of 40 U/ml trypsin solution was added, and blended again, then incubated at 39 °C and 150 r/min for 4 h. After digestion was completed, the digested suspension was centrifuged at 4000 r/min (1200×g) for 15 min. The sediment obtained was oven dried for nutrient assay. In vitro nutrient digestibility (%)=(original nutrient amount–residual nutrient amount)/original nutrient amount×100%. Statistical analysis 2 One-way analysis of variance was performed using the General Linear Models Procedures of the SAS software (SAS, 1999 1999 ). Differences among means were tested using Duncan’s multiple range tests. A significance level of 0.05 was used. RESULTS AND DISCUSSION 1 Residual free gossypol and detoxification efficiency 2 Residual FG levels of fermented CSM substrate from different treatments were significantly lower ( P < 0.05) than control (substrate), indicating fermentation could decrease the FG content of CSM (Table 1 1 ). Means in a column without common superscript differ significantly ( P < 0.05) Treatment FG (mg/(kg DM)) Detoxification efficiency (%) CP (%DM) CP increase percentage (%) Substrate (control) 549.06±9.87 a − 23.79±0.11 c − C . capsuligena ZD-1 145.51±10.02 c 73.50 25.92±0.23 b 8.95 C . tropicalis ZD-3 29.80±5.28 f 94.57 26.35±0.34 b 10.76 S . cerevisae ZD-5 63.07±7.38 e 88.51 26.43±0.09 b 11.10 A . terricola ZD-6 93.87±9.93 d 82.91 26.21±0.17 b 10.17 A . oryzae ZD-7 178.39±8.86 b 67.51 26.04±0.14 b 9.46 A . niger ZD-8 81.50±4.77 d 85.16 29.08±0.21 a 22.24 The effect from the microbial species on FG contents could be differentiated statistically ( P < 0.05) as follows: C . tropicalis ZD-3 fermented CSM had lowest level, the amount of FG being found to be 29.8 mg/kg based on DM, detoxification efficiency reaching up to 94.57%; followed by S . cerevisae ZD-5, A . niger ZD-8 and A . terricola ZD-6, of which the FG levels in fermented substrate were up to 63.07, 81.50 and 93.87 mg/kg in DM respectively. Their detoxification efficiency reached 88.51%, 85.16% and 82.91% respectively. Although the effect of C . capsuligena ZD-1 and A . oryzae ZD-7 treatments was not better than those of other microflora, FG contents were reduced significantly ( P < 0.05) to safety level. The effect of this decrease may have been caused by the binding of FG to protein or amino acids secreted by microorganisms, or by introducing microbial enzymes degrading gossypol, or by both. Crude protein determination 2 Table 1 1 presents the results of CP determination. It is evident from the results that CSM fermentation by different microbial strains improved CP content significantly ( P < 0.05), CP increased from 8.95% to 22.24%. Of all fungal treatments, A . niger ZD-8 fermentation efficiency was highest, CP content was increased by 22.24%. However, the contribution to CP increase of C . capsuligena ZD-1, C . tropicalis ZD-3, S . cerevisae ZD-5, A . terricola ZD-6 and A . oryzae ZD-7 did not differ significantly from each other. The additional amount of CP in CSM substrate was mainly due to the growth of microflora. Microbes converted substrate protein and other nutrients into microbial cell protein, secreted enzymes, and other biological products, consumed carbohydrate to supply energy, and meanwhile released CO 2 and H 2 O, as well as some volatile materials, thus leading to a CP content increase per unit. Among the six fungal strains used in this study, were three strains belonging to yeast, i.e., C . capsuligena ZD-1, C . tropicalis ZD-3 and S . cerevisae ZD-5; and three strains belonging to filamentous fungi, i.e., A . terricola ZD-6, A . oryzae ZD-7 and A . niger ZD-8. Among the three yeast strains, detoxification efficiency and CP increase were highest for C . tropicalis ZD-3. The detoxification characteristics were according to Shi et al.( 1998 1998 ) and Yang et al.( 2000 2000 ), whereas the fermentation of C . tropicalis ZD-3 was more efficient. S . cerevisae ZD-5 is widely used in the feed fermentation industry, however, our trial results showed that S . cerevisae ZD-5 had better detoxification efficiency, although not more efficient than C . tropicalis ZD-3. It could decrease FG level in CSM substrate up to 63.07 mg/kg, which is far less than the restricted level of 100 mg/kg in swine feed. Therefore, S . cerevisae ZD-5 could be also applied for detoxification of CSM. The strain ZD-1 of C . capsuligena is mainly used in feed yeast production employing decomposed cellulose. It had ordinary effect on gossypol detoxification. The results presented above suggest that strain ZD-3 of C . tropicalis would be the first choice for microfloral detoxification of gossypol followed by S . cerevisae ZD-5. Concerning filamentous fungi, the present results showed that A . niger ZD-8 was far more effective than the other two strains. It not only had a higher detoxification efficiency, but also had higher CP increase during solid fermentation of CSM. A characteristic of A . niger ZD-8 was that it had a good smell during CSM fermentation, and had a faster growth rate, and so is fit for fermenting CSM to reduce FG concentration. The strain of A . terricola ZD-6 had relatively good detoxification ability, and improving protein quality was common, and it does not have a good smell during fermentation. It could grew well on Czapek’s medium containing 0.5% gossypol instead of sucrose as carbon source, therefore, A . terricola ZD-6 was a good biological engineering species for investigating the enzyme needed for gossypol degradation. The detoxification efficiency of strain A . oryzae ZD-7 was lower and it was not fit for fermentation detoxification. The results in the present study demonstrated that C . tropicalis ZD-3 and A . niger ZD-8 are two better strains for fermentation detoxification of FG in CSM. Amino acids assay of CSM fermented by C . tropicalis ZD-3 and A . niger ZD-8 2 Amino acids content of CSM fermented by C . tropicalis ZD-3 and A . niger ZD-8 is presented in Table 2 2 . The results showed that the TAA (total amino acids) and EAA of fermented CSM were increased significantly ( P < 0.05) compared with negative control, the TAA of substrate fermented by C . tropicalis ZD-3 and A . niger ZD-8 were improved by 7.06% and 11.46%, and the EAA were increased by 7.77% and 12.64%, respectively. Especially, the levels of methionine, lysine and threonine were improved greatly ( P < 0.05), and the effectiveness of A . niger ZD-8 was superior to that of C . tropicalis ZD-3. Means in a row without common superscript differ significantly ( P < 0.05); DM: Dry matter; TAA: Total amino acids; EAA: Essential amino acids The amino acid is an essential amino acid Nutrition composition Substrate (control, C ) C . tropicalis ZD-3 ( T 1 ) ( T 1 − C )/ C ×100% A . niger ZD-8 ( T 2 ) ( T 2 − C )/ C ×100% DM 89.87 88.63 − 88.15 − Asp 2.35 a 2.35 a 0 2.47 a 5.28 Thr * * 0.82 b 0.95 a 15.56 1.02 a 24.31 Ser 1.05 b 1.16 a 10.32 1.22 a 15.85 Glu 5.29 a 5.58 a 5.46 5.38 a 1.74 Gly 1.04 b 1.15 a 10.40 1.24 a 18.63 Ala 1.11 b 1.27 b 13.88 1.51 a 35.68 Cys * * 0.50 a 0.52 a 4.05 0.58 a 16.78 Val * * 1.03 b 1.17 a 13.54 1.28 a 24.12 Met * * 0.22 c 0.30 b 33.11 0.35 a 57.36 Ile * * 0.76 c 0.89 b 17.15 1.06 a 40.43 Leu * * 1.61 c 1.82 b 13.10 2.01 a 24.80 Tyr 0.66 b 0.73 a 10.61 0.82 a 24.20 Phe * * 1.20 b 1.40 a 16.24 1.34 a 10.91 Lys * * 1.04 b 1.16 a 11.20 1.10 ab 5.57 His * * 0.60 a 0.62 a 3.25 0.64 a 6.25 Arg * * 2.65 a 2.42 b −8.46 2.37 b −10.35 Pro 0.86 b 0.93 a 8.23 1.02 a 18.79 TAA 22.80 b 24.41 ab 7.06 25.41 a 11.46 EAA 10.44 b 11.25 a 7.77 11.76 a 12.64 In vitro CP and AA digestibility of CSM fermented by C . tropicalis ZD-3 and A . niger ZD-8 2 The results of in vitro CP and AA digestibility of CSM fermented by C . tropicalis ZD-3 and A . niger ZD-8 are presented in Table 3 3 . The results demonstrated that in vitro CP and AA digestibility of fermented CSM was increased significantly ( P < 0.05) compared with control, the in vitro CP digestibility of CSM fermented by C . tropicalis ZD-3 and A . niger ZD-8 was improved by 13.42% and 18.22%, the TAA were increased by 17.75% and 22.88%, and the EAA by 16.61% and 21.01%, respectively. In addition, the in vitro digestibility of methionine, lysine and threonine was also improved greatly ( P < 0.05). The experimental results suggested that the fermentation efficiency and digestibility of A . niger ZD-8 was superior to that of C . tropicalis ZD-3. Means in a row without common superscript differ significantly ( P < 0.05); DM: Dry matter; TAA: Total amino acids; EAA: Essential amino acids The amino acid is an essential amino acid Nutrition composition Substrate (control, C ) C . tropicalis ZD-3 ( T 1 ) ( T 1 − C )/ C ×100% A . niger ZD-8 ( T 2 ) ( T 2 − C )/ C ×100% CP 44.79 c 50.80 b 13.42 52.95 a 18.22 Asp 41.38 b 54.22 a 31.03 56.83 a 37.34 Thr * * 65.74 b 74.91 a 13.95 77.06 a 17.22 Ser 40.48 b 52.11 a 28.73 53.56 a 32.31 Glu 38.76 b 48.04 a 23.94 49.94 a 28.84 Gly 34.44 c 49.17 a 42.77 51.67 a 50.03 Ala 52.58 a 52.34 a −0.46 54.53 a 3.71 Cys * * 44.62 c 49.96 b 11.97 52.87 a 18.49 Val * * 49.08 a 49.65 a 1.16 50.88 a 3.67 Met * * 49.71 b 65.38 a 31.52 67.05 a 34.88 Ile * * 39.09 c 49.33 b 26.20 52.09 a 33.26 Leu * * 38.25 c 46.89 b 22.59 49.12 a 28.42 Tyr 44.29 c 49.28 b 11.27 51.55 a 16.39 Phe * * 37.73 b 47.97 a 27.14 49.73 a 31.80 Lys * * 50.08 c 58.04 b 15.89 61.17 a 22.14 His * * 51.66 c 62.80 b 21.56 64.36 a 24.58 Arg * * 68.98 b 72.13 ab 4.57 74.54 a 8.06 Pro 31.69 c 34.62 b 9.25 39.85 a 25.75 TAA 45.80 c 53.93 a 17.75 56.28 a 22.88 EAA 49.49 b 57.71 a 16.61 59.89 a 21.01 CONCLUSION 1 From the results of the present study it could be concluded that microbial fermentation is instrumental in reducing FG levels in CSM. 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Selection and study of high effective microbiology for digesting free gossypol and proper fermentation processing data for detoxification in cottonseed cakes The authors wish to express their gratitude to Prof. Li Weifen, Ph.D., Yan Xianghua, Wang Yanbo, Ma Jifeng, Jiang Junfang, Tao Xin and Huang Qichun for skillful technical assistance. 10.1631/jzus.2006.B0690 Project (No. 30471255) supported by the National Natural Science Foundation of China 2005-08-24 2006-02-21 2006-08-15 2022-01-05 Copyright © 2006, Journal of Zhejiang University Science 2006 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 09 07