基于<italic style="font-style: italic">gN</italic>基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用

金玉兰; 季暄; 董伟仁; 黄彦明; 周继勇; 顾金燕

doi:10.1631/jzus.B2500729

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基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用

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Correspondence | Updated：2026-08-13

基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用
Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2026年27卷第8期页码：923-926
Affiliations：

1.The Experimental Teaching Center, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
2.MOA Key Laboratory of Animal Virology, Zhejiang University Center for Veterinary Sciences, Hangzhou 310058, China
Funds：

the Hangzhou Chengxi Sci-Tech Innovation Corridor Management Committee(B231220.0005-46);the National Key Research and Development Plan of China(2022YFD1800804)
DOI：10.1631/jzus.B2500729
中图分类号：
CSTR：
收稿：2025-11-13，

修回：2026-02-24，

网络首发：2026-08-13，

纸质出版：2026-08-13
Accepted：

金玉兰,季暄,董伟仁等.基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(08):923-926.

Yulan JIN, Xuan JI, Weiren DONG, et al. Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(8): 923-926. DOI: 10.1631/jzus.B2500729.
金玉兰,季暄,董伟仁等.基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(08):923-926. DOI： 10.1631/jzus.B2500729.

Yulan JIN, Xuan JI, Weiren DONG, et al. Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(8): 923-926. DOI: 10.1631/jzus.B2500729. DOI：

摘要

伪狂犬病是由伪狂犬病病毒（PRV）引起的一种严重危害全球养猪业的传染病，由于不同基因型毒株的致病性与流行特征存在差异，临床上需建立一种能够快速、准确区分PRV基因I型和基因II型的分型检测方法。本研究首次以

基因为靶标，基于其保守区域鉴别稳定差异序列，成功建立了一种TaqMan三重实时荧光定量聚合酶链式反应（PCR）检测方法。该方法不仅可检测PRV感染，而且能有效区分其基因型。该检测方法具有特异性强、灵敏度高、重复性好及操作简便等优点。该检测体系仅需一对引物和三个探针即可完成分型鉴定，极大简化了反应体系，降低了引物错配及非特异性扩增的风险。对实验动物来源样本初步应用的结果显示，该方法能准确检测PRV的感染情况及其基因型。本研究为PRV的分子流行病学监测提供了实用、高效的技术工具，也为猪群中PRV的净化工作提供了有力支持。

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基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用

Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus

DOI：10.1631/jzus.B2500729

摘要

Abstract

关键词

Keywords

references