一种具有生物相容性和机械强度的新型多组分协同生物墨水用于促进软骨再生

马梓钧; 贾雯丹; 王晓圆; 成荣; 韩璐; 李梦; 杨晓宁; 桑胜波

doi:10.1631/jzus.B2500343

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一种具有生物相容性和机械强度的新型多组分协同生物墨水用于促进软骨再生

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Research Article | Updated：2025-12-31

一种具有生物相容性和机械强度的新型多组分协同生物墨水用于促进软骨再生
Novel multi-component synergistic bioink that balances biocompatibility and mechanical strength for cartilage regeneration
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2025年26卷第12期页码：1156-1171
Affiliations：

1.Shanxi Key Laboratory of Artificial Intelligence & Micro Nano Sensors, College of Integrated Circuits, Taiyuan University of Technology, Taiyuan 030024, China
2.Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China
3.Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030024, China
Funds：

the Project(JCKY2024408C010);the Shanxi Province Key Research and Development Project(202302130501006);the National Natural Science Foundation of China(82403350;51975400;62031022);the Shanxi Provincial Key Medical Scientific Research Project(2020XM06);the Shanxi Provincial Basic Research Project(202103021223040);the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2021L044);the Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(2022SX-TD026)
DOI：10.1631/jzus.B2500343
中图分类号：
CSTR：
收稿：2025-06-17，

修回：2025-10-04，

纸质出版：2025-12-15
Accepted：

马梓钧,贾雯丹,王晓圆等.一种具有生物相容性和机械强度的新型多组分协同生物墨水用于促进软骨再生[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(12):1156-1171.

Zijun MA, Wendan JIA, Xiaoyuan WANG, et al. Novel multi-component synergistic bioink that balances biocompatibility and mechanical strength for cartilage regeneration[J]. Journal of Zhejiang University-SCIENCE B, 2025, 26(12): 1156-1171.
马梓钧,贾雯丹,王晓圆等.一种具有生物相容性和机械强度的新型多组分协同生物墨水用于促进软骨再生[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(12):1156-1171. DOI： 10.1631/jzus.B2500343.

Zijun MA, Wendan JIA, Xiaoyuan WANG, et al. Novel multi-component synergistic bioink that balances biocompatibility and mechanical strength for cartilage regeneration[J]. Journal of Zhejiang University-SCIENCE B, 2025, 26(12): 1156-1171. DOI： 10.1631/jzus.B2500343.

摘要

水凝胶因其类似于细胞外基质的多孔网络结构，已成为软骨组织工程领域必不可少的支架材料。其中，甲基丙烯酸明胶（GelMA）水凝胶因其优异的生物相容性、生物降解性和可调的光交联性能而广泛应用于生物墨水开发。然而，纯GelMA的高生物相容性往往以机械强度为代价，限制了其在软骨再生中的适用性。为了克服这种制约关系，本研究开发了基于GelMA、丝素蛋白（SF）和聚环氧乙烷（PEO）的复合生物墨水，用于制备多孔水凝胶支架，然后从形貌、孔隙率、亲水性、机械强度、流变行为、可印刷性和细胞相容性等方面对其进行了系统表征。在这种设计中，PEO作为致孔剂产生高度多孔的结构（孔隙率高达88%），而SF补偿了PEO造成的机械损失，使支架能够保持高达29.10 kPa的压缩强度。在测试的配方中，10% GelMA/1% SF/1.5% PEO（1%=0.01 g/mL）生物墨水表现出优异的打印性能、机械性能和细胞相容性，并且在打印后支持软骨细胞对II型胶原和聚集蛋白聚糖的牢固沉积。这项工作为平衡生物墨水的生物相容性和机械性能提供了一种多功能策略，为下一代软骨组织工程支架提供了一个有前景的平台。

Abstract

Hydrogels

owing to their porous network structure resembling the extracellular matrix (ECM)

have become essential scaffold materials in the field of cartilage tissue engineering. Among them

gelatin methacrylate (GelMA) hydrogels are widely used in bioink development due to their excellent biocompatibility

biodegradability

and tunable photo-crosslinking properties. However

the high biocompatibility of pure GelMA often comes at the cost of mechanical strength

limiting its applicability in cartilage regeneration. To overcome this trade-off

this study developed composite bioinks based on GelMA

silk fibroin (SF)

and polyethylene oxide (PEO) for fabricating porous hydrogel scaffolds

which were then systematically characterized in terms of morphology

porosity

hydrophilicity

mechanical strength

rheological behavior

printability

and cytocompatibility. In this design

PEO serves as a porogen to generate highly porous structures (porosity up to 88%)

while SF compensates for the mechanical loss caused by PEO

enabling the scaffold to retain a compression strength of up to 29.10 kPa. Among the tested formulations

the 10% GelMA/1% SF/1.5% PEO (1%=0.01 g/mL) bioink exhibited excellent printability

mechanical integrity

and cytocompatibility

and it supported a robust deposition of collagen II and aggrecan by chondrocytes after printing. This work provides a versatile strategy for balancing the biocompatibility and mechanical robustness in bioinks

offering a promising platform for next-generation cartilage tissue engineering scaffolds.

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references

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