circBDP1中A-G的新突变可影响牛脂肪细胞的增殖分化及胴体性状

蒋恩惠; 张驰原; 何卓远; 张彧丽; 杨钰塔; 潘传英; 姜富贵; 宋恩亮; 张思欢; 蓝贤勇

doi:10.1631/jzus.B2500084

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circBDP1中A-G的新突变可影响牛脂肪细胞的增殖分化及胴体性状

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Correspondence | Updated：2026-08-13

circBDP1中A-G的新突变可影响牛脂肪细胞的增殖分化及胴体性状
A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2026年27卷第8期页码：918-922
Affiliations：

1.College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
2.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
3.Institute of Animal Science and Veterinary, Shandong Academy of Agriculture Science, Jinan 250100, China
Funds：

the National Natural Science Foundation of China(32372852);the Science Fund for Distinguished Young Scholars of Shaanxi Province(2024JC-JCQN-30);the Shaanxi Provincial Innovation Leadership Program in Sciences and Technologies for Young and Middle-aged Scientists(2023SR205);the Project of Undergraduate Science and Technology Innovation Items of Northwest A&F University(202410712014)
DOI：10.1631/jzus.B2500084
中图分类号：
CSTR：
收稿：2025-02-23，

修回：2025-05-08，

网络首发：2026-08-13，

纸质出版：2026-08-13
Accepted：

蒋恩惠,张驰原,何卓远等.circBDP1中A-G的新突变可影响牛脂肪细胞的增殖分化及胴体性状[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(08):918-922.

Enhui JIANG, Chiyuan ZHANG, Zhuoyuan HE, et al. A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(8): 918-922.
蒋恩惠,张驰原,何卓远等.circBDP1中A-G的新突变可影响牛脂肪细胞的增殖分化及胴体性状[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(08):918-922. DOI： 10.1631/jzus.B2500084.

Enhui JIANG, Chiyuan ZHANG, Zhuoyuan HE, et al. A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(8): 918-922. DOI： 10.1631/jzus.B2500084.

摘要

脂肪沉积是影响牛生长发育及肉产量和品质的重要因素。前期发现环状RNA circBDP1可促进牛脂肪细胞的增殖与分化。本研究通过鉴定circBDP1基因（rs454813519）上的A>G突变，采用Sanger测序与Kompetitive Allele-Specific PCR（KASP）技术，在秦川牛及山东黑牛遗传资源群体（Shandong Black Cattle Genetic Resource

SDBCGR）中检测到circBDP1-SNP位点的三种基因型（AA、AG和GG）。关联分析结果表明，该circBDP1-SNP A>G变异与SDBCGR母牛胸肌、上脑、牛肋排等性状和公牛胴体重、秦川牛体长等性状相关。为揭示该突变的相关功能，本研究构建了携带circBDP1-SNP不同等位基因的circBDP1-G/A过表达载体，并转染至牛前体脂肪细胞中。EdU检测、流式细胞术及qRT-PCR等实验结果显示，与circBDP1-G相比，circBDP1-A可显著增强前脂肪细胞的增殖与分化。此外，据已发表的全球牛基因组数据推测，G等位基因可能源自印度瘤牛。综上，circBDP1基因上的circBDP1-SNP A>G突变影响前脂肪细胞增殖与分化，可作为肉牛分子辅助选择的DNA标记。

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