气液相等离子体诱变技术结合高通量筛选提升裂殖壶菌中二十碳五烯酸产量的应用

余超; 朱佳林; 吴金勇; 陈祥松; 陆姝欢; 李翔宇; 赵洒; 朱薇薇; 舒敏; 吴绵斌; 姚建铭

doi:10.1631/jzus.B2400427

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气液相等离子体诱变技术结合高通量筛选提升裂殖壶菌中二十碳五烯酸产量的应用

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Research Article | Updated：2026-03-18

气液相等离子体诱变技术结合高通量筛选提升裂殖壶菌中二十碳五烯酸产量的应用
Integrative combination of gas‒liquid-phase plasma mutagenesis and high-throughput screening to enhance eicosapentaenoic acid production by Schizochytrium sp.
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2026年27卷第3期页码：250-263
Affiliations：

1.University of Science and Technology of China, Hefei 230026, China
2.Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
3.Cabio Biotech (Wuhan) Biotechnology Co., Ltd., Wuhan 430223, China
4.Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310030, China
5.Ningbo Global Innovation Center, Zhejiang University, Ningbo 315100, China
Funds：

the National Key Research and Development Program of China(2021YFC2100800)
DOI：10.1631/jzus.B2400427
中图分类号：
CSTR：
收稿：2024-08-19，

修回：2024-10-13，

网络首发：2025-12-22，

纸质出版：2026-03-18
Accepted：

余超,朱佳林,吴金勇等.气液相等离子体诱变技术结合高通量筛选提升裂殖壶菌中二十碳五烯酸产量的应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(03):250-263.

Chao YU, Jialin ZHU, Jinyong WU, et al. Integrative combination of gas‒liquid-phase plasma mutagenesis and high-throughput screening to enhance eicosapentaenoic acid production by Schizochytrium sp.[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(3): 250-263.
余超,朱佳林,吴金勇等.气液相等离子体诱变技术结合高通量筛选提升裂殖壶菌中二十碳五烯酸产量的应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(03):250-263. DOI： 10.1631/jzus.B2400427.

Chao YU, Jialin ZHU, Jinyong WU, et al. Integrative combination of gas‒liquid-phase plasma mutagenesis and high-throughput screening to enhance eicosapentaenoic acid production by Schizochytrium sp.[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(3): 250-263. DOI： 10.1631/jzus.B2400427.

摘要

膳食中摄入二十碳五烯酸（eicosapentaenoic acid，EPA）可带来多种健康益处，如调节血液中甘油三酯水平和预防心血管疾病。尽管裂殖壶菌（

Schizochytrium

sp.）具备天然合成EPA的能力，但其产量偏低，这导致裂殖壶菌无法在工业化生产中得到广泛应用。因此，本研究利用气液相等离子体诱变技术与高通量筛选相融合的研究策略，有效提高了裂殖壶菌生产EPA的效率。首先，利用气液相等离子体诱变技术获得一系列裂殖壶菌突变株，并利

用近红外光谱（NIRS）技术快速准确地筛选出高产EPA的突变株M7-25，同时遗传稳定性实验表明该突变株生产稳定性良好。经过对培养基配方的优化，M7-25的EPA产量从0.45 g/L显著提升至1.70 g/L。在此基础上，通过调整氨水和葡萄糖的联合补料策略，在7 L发酵罐中，EPA产量达到2.08 g/L。这是目前利用诱变技术在裂殖壶菌中报道的最高EPA产量，彰显了其在工业化生产中具有较大潜在应用价值。

Abstract

Dietary consumption of eicosapentaenoic acid (EPA) offers diverse health benefits

such as the regulation of blood triglycerides and the prevention of cardiovascular diseases. EPA is naturally synthesized by

Schizochytrium

sp.; however

its low production level limits its potential for industrial application. The goal of this study was to increase EPA productivity in

Schizochytrium

sp. by gas‒liquid-phase plasma (GLPP) mutagenesis combined with a high-throughput screening method. First

a diverse array of mutants was generated through GLPP mutagenesis. Next

the mutants with elevated EPA productivity were identified through near-infrared spectroscopy (NIRS). Notably

the M7-25 mutant demonstrated the highest and most consistent EPA production. After the culture medium was optimized

the EPA titer increased from 0.45 to 1.70 g/L. Finally

a cofermentation strategy using ammonia and glucose feeding was employed

and the EPA titer reached 2.08 g/L in a 7-L fermenter. This study reports the highest EPA titer achieved in

Schizochytrium

sp. via mutagenesis to date

highlighting its great market potential for industrial production.

关键词

Keywords

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