刚地弓形虫三种线粒体Mg<sup>2+</sup>/Mn<sup>2+</sup>依赖性蛋白磷酸酶（PPMs）存在功能冗余

盛楷茵; 陈学秋; 阳毅敏; 夏洁; 宋凯悦; Chaoqun YAO; 杨怡; 杜爱芳; 马光旭

doi:10.1631/jzus.B2400308

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刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余

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Correspondence | Updated：2025-04-23

刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余
Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2025年26卷第4期页码：405-408
Affiliations：

1.College of Animal Sciences, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China
2.ZJU-Xinchang Joint Innovation Centre (Tianmu Laboratory), Gaochuang Hi-Tech Park, Xinchang 312500, China
3.Department of Biomedical Sciences and One Health Center for Zoonoses and Tropical Infectious Diseases, Ross University School of Veterinary Medicine, Basseterre KN-0101, The Federation of Saint Kitts and Nevis
Funds：

the National Natural Science Foundation of China(31672543);the Zhejiang Province “Sannongliufang” Science and Technology Cooperation Project(2020SNLF007)
DOI：10.1631/jzus.B2400308
中图分类号：
CSTR：
收稿日期：2024-06-18，

修回日期：2024-09-06，

纸质出版日期：2025-04-23
Accepted：

盛楷茵,陈学秋,阳毅敏等.刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(04):405-408.

Kaiyin SHENG, Xueqiu CHEN, Yimin YANG, et al. Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(4): 405-408.
盛楷茵,陈学秋,阳毅敏等.刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(04):405-408. DOI： 10.1631/jzus.B2400308.

Kaiyin SHENG, Xueqiu CHEN, Yimin YANG, et al. Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(4): 405-408. DOI： 10.1631/jzus.B2400308.

摘要

Abstract

弓形虫（

Toxoplasma gondii

）是一种专性胞内寄生的顶复门原虫，可以感染包括人在内的几乎所有温血动物，引起全球性的人畜共患弓形虫病，从而危害人类健康和畜牧业发展，并造成经济损失。刚地弓形虫独特的线粒体是弓形虫病防控的理想靶细胞器。线粒体中的Mg

/Mn

依赖性蛋白磷酸酶（PPMs）在细胞生长、能量代谢和环境应激中发挥着重要的作用。然而，人们对弓形虫的线粒体PPMs知之甚少。在本研究中，我们鉴定出三种PPMs，即

PPM8（TGGT1_218590）、

PPM10（TGGT1_254410）和

PPM19（TGGT1_275840），均定位于弓形虫速殖子的线粒体基质中。这三个PPMs中任一PPM编码基因的缺失（Δ

ppm8

、Δ

ppm10

或Δ

ppm19

）均会导致弓形虫速殖子生长抑制与增殖能力减弱，以及另外两个PPMs编码基因信使RNA（mRNA）水平的升高，但不影响弓形虫速殖子在小鼠体内的毒力。Δ

ppm8

ppm10

或Δ

ppm8

ppm19

双重缺失可显著导致弓形虫速殖子体外生长抑制与增殖减缓。由于潜在的突变致死效应，本研究未获得Δ

ppm10

ppm19

和Δ

ppm8

ppm10

ppm19

缺失虫株。上述结果表明，弓形虫线粒体PPMs间存在功能冗余，是弓形虫线粒体生物学研究的新发现，为具有重要兽医和医学意义的弓形虫的防控靶点的发掘提供了新思路。

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Keywords

references

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刚地弓形虫三种线粒体Mg2+/Mn2+依赖性蛋白磷酸酶（PPMs）存在功能冗余

Functional redundancy of three mitochondrial Mg2+/Mn2+-dependent protein phosphatases (PPMs) in Toxoplasma gondii

DOI：10.1631/jzus.B2400308

摘要

Abstract

关键词

Keywords

references

刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余

Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii