一株去除废水中氨氮的新型细菌：油橄榄假单胞菌QS-7

邱浩; 廖敏; 谢晓梅; 陆欣悦; 袁枫; 罗哲; 樊春林

doi:10.1631/jzus.B2400257

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Research Article | Updated：2026-03-18

一株去除废水中氨氮的新型细菌：油橄榄假单胞菌QS-7
A novel bacterial strain for the removal of ammonia nitrogen from wastewater: Pseudomonas oleovorans QS-7
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2026年27卷第3期页码：236-249
Affiliations：

1.College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China
2.National Demonstration Center for Experimental Environmental and Resources Education (Zhejiang University), Hangzhou 310058, China
3.Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Hangzhou 310058, China
4.Ningbo Ferno Biotechnology Co., Ltd., Ningbo 315502, China
Funds：
DOI：10.1631/jzus.B2400257
中图分类号：
CSTR：
收稿：2024-05-21，

修回：2024-10-19，

网络首发：2025-11-24，

纸质出版：2026-03-18
Accepted：

邱浩,廖敏,谢晓梅等.一株去除废水中氨氮的新型细菌：油橄榄假单胞菌QS-7[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(03):236-249.

Hao QIU, Min LIAO, Xiaomei XIE, et al. A novel bacterial strain for the removal of ammonia nitrogen from wastewater: Pseudomonas oleovorans QS-7[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(3): 236-249.
邱浩,廖敏,谢晓梅等.一株去除废水中氨氮的新型细菌：油橄榄假单胞菌QS-7[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(03):236-249. DOI： 10.1631/jzus.B2400257.

Hao QIU, Min LIAO, Xiaomei XIE, et al. A novel bacterial strain for the removal of ammonia nitrogen from wastewater: Pseudomonas oleovorans QS-7[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(3): 236-249. DOI： 10.1631/jzus.B2400257.

摘要

过量氨氮对水环境构成严重的威胁。具有同步硝化反硝化（SND）功能的细菌，是实现污水中氨氮高效去除的有效生物工具。本文首次报道从养猪场沼气处理系统中分离得到的油橄榄假单胞菌（

Pseudomonas oleovorans

）QS-7具有SND功能，能够高效去除水中氨氮。通过检测氮代谢相关的关键酶、功能基因以及氮代谢过程的氮平衡实验，我们推测该菌株QS-7的SND途径为：

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110658&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110657&type=

5.75733376

3.47133350

→NH

OH→

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110637&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110619&type=

5.67266655

3.47133350

→

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110683&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110662&type=

5.67266655

3.47133350

→

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110637&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110619&type=

5.67266655

3.47133350

→NO→N

O→N

。实验表明，菌株QS-7在100 mg/L氨氮培养基中，最大去除率达98.6%，且在18小时内的最大氨氮降解率为9.2 mg/(L∙h)。该菌株对硝态氮和亚硝态氮也表现出一定的去除能力，在以二者为唯一氮源（100 mg/L）的体系中，最大去除率分别为54.22%和73.93%。以氨氮（100 mg/L）为唯一氮源的氮代谢平衡实验中，同化作用（56.1%）是去除氨氮的主要方式，其次是转化为N

（43.6%）。同时，反应过程中未检测到

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110671&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110664&type=

5.67266655

3.47133350

的存在，且NO

的产量极低，这表明QS-7的脱氨过程环境友好。研究进一步确定QS-7的最佳环境条件是：碳源为柠檬酸钠，C/N=10，pH=7.0，摇床转速150 r/min，温度30 ℃。上述结果表明，菌株QS-7可为SND技术的发展提供菌种资源与理论依据。

Abstract

Excessive ammonia nitrogen has been demonstrated to cause a serious hazard to water environments. Bacteria performing simultaneous nitrification and denitrification (SND) can be effective biological instruments to remove ammonia nitrogen completely from effluents. For the first time

Pseudomonas oleovorans

QS-7 with SND function

isolated from the biogas treatment system of a pig farm

was found to efficiently remove ammonia nitrogen. Through the determination of key enzymes and functional genes related to the nitrogen metabolism of strain QS-7

combined with nitrogen balance measurements of the nitrogen metabolic process

it was speculated that the SND pathway of the novel strain is

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110639&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110627&type=

5.75733376

3.47133350

→NH

OH→

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110640&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110613&type=

5.67266655

3.47133350

→

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110632&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110631&type=

5.67266655

3.47133350

→

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110640&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110613&type=

5.67266655

3.47133350

→NO→N

O→N

. QS-7 exhibited 98.6% ammonia nitrogen removal and a maximum ammonia degradation rate of 9.2 mg/(L·h) at 18 h in 100 mg/L ammonia nitrogen solution. This strain also has a certain capacity to remove nitrate and nitrite nitrogen; the maximum removal efficiencies were 54.22% and 73.93%

respectively

in systems with 100 mg/L of nitrate or nitrite nitrogen as

the sole nitrogen source. Nitrogen metabolic balance analysis for QS-7

using ammonia (100 mg/L) as the sole nitrogen source

demonstrated that assimilation (56.1%) is the main mode of nitrogen removal

followed by conversion to N

(43.6%). Meanwhile

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110640&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110613&type=

5.67266655

3.47133350

was not detected

and almost no NO

was produced

which indicates that the nitrogen removal process of QS-7 is environmentally friendly. The optimal environmental conditions for QS-7 were found to be sodium citrate as the carbon source

C/N=10

pH=7.0

150 r/min

and 30 ℃. The above results indicate that QS-7 may provide a material and conceptual basis for the advancement of SND technology.

关键词

Keywords

references

Chen MP , He TX , Wu QF , et al. , 2023 . Enhanced heterotrophic nitrification and aerobic denitrification performance of Glutamicibacter arilaitensis EM-H8 with different carbon sources . Chemosphere , 323 : 138266 . https://doi.org/10.1016/j.chemosphere.2023.138266 https://doi.org/10.1016/j.chemosphere.2023.138266

Chen XL , Li SF , Zhang WX , et al. , 2024 . A newly isolated Rhodococcus sp. S2 from landfill leachate capable of heterotrophic nitrification and aerobic denitrification . Water , 16 ( 3 ): 431 . https://doi.org/10.3390/w16030431 https://doi.org/10.3390/w16030431

Cui Y , Cui YW , Huang JL , 2021 . A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification . Bioresour Technol , 333 : 125189 . https://doi.org/10.1016/j.biortech.2021.125189 https://doi.org/10.1016/j.biortech.2021.125189

Dong YT , Yuan HP , Zhang RN , et al. , 2019 . Removal of ammonia nitrogen from wastewater: a review . Trans ASABE , 62 ( 6 ): 1767 - 1778 . https://doi.org/10.13031/trans.13671 https://doi.org/10.13031/trans.13671

Gao Y , Zhu JW , Wang KY , et al. , 2023 . Discovery of a heterotrophic aerobic denitrification Pseudomonas sp. G16 and its unconventional nitrogen metabolic pathway . Bioresour Technol , 387 : 129670 . https://doi.org/10.1016/j.biortech.2023.129670 https://doi.org/10.1016/j.biortech.2023.129670

Ge QL , Yue XP , Wang GY , 2015 . Simultaneous heterotrophic nitrification and aerobic denitrification at high initial phenol concentration by isolated bacterium Diaphorobacter sp. PD-7 . Chin J Chem Eng , 23 ( 5 ): 835 - 841 . https://doi.org/10.1016/j.cjche.2015.02.001 https://doi.org/10.1016/j.cjche.2015.02.001

Gui MY , Chen Q , Ni JR , 2017 . Effect of sulfamethoxazole on aerobic denitrification by strain Pseudomonas stutzeri PCN-1 . Bioresour Technol , 235 : 325 - 331 . https://doi.org/10.1016/j.biortech.2017.03.131 https://doi.org/10.1016/j.biortech.2017.03.131

He TX , Xie DT , Ni JP , et al. , 2020 . Nitrous oxide produced directly from ammonium, nitrate and nitrite during nitrification and denitrification . J Hazard Mater , 388 : 122114 . https://doi.org/10.1016/j.jhazmat.2020.122114 https://doi.org/10.1016/j.jhazmat.2020.122114

He TX , Chen MP , Ding CY , et al. , 2021 . Hypothermia Pseudomonas taiwanensis J488 exhibited strong tolerance capacity to high dosages of divalent metal ions during nitrogen removal process . Bioresour Technol , 341 : 125785 . https://doi.org/10.1016/j.biortech.2021.125785 https://doi.org/10.1016/j.biortech.2021.125785

Hu B , Lu JY , Qin YX , et al. , 2023 . A critical review of heterotrophic nitrification and aerobic denitrification process: influencing factors and mechanisms . J Water Process Eng , 54 : 103995 . https://doi.org/10.1016/j.jwpe.2023.103995 https://doi.org/10.1016/j.jwpe.2023.103995

Huang F , Pan LQ , Lv N , et al. , 2017 . Characterization of novel Bacillus strain N31 from mariculture water capable of halophilic heterotrophic nitrification-aerobic denitrification . J Biosci Bioeng , 124 ( 5 ): 564 - 571 . https://doi.org/10.1016/j.jbiosc.2017.06.008 https://doi.org/10.1016/j.jbiosc.2017.06.008

Huang XF , Li WG , Zhang DY , et al. , 2013 . Ammonium removal by a novel oligotrophic Acinetobacter sp. Y16 capable of heterotrophic nitrification-aerobic denitrification at low temperature . Bioresour Technol , 146 : 44 - 50 . https://doi.org/10.1016/j.biortech.2013.07.046 https://doi.org/10.1016/j.biortech.2013.07.046

Huang XJ , Xu Y , He TX , et al. , 2019 . Ammonium transformed into nitrous oxide via nitric oxide by Pseudomonas putida Y-9 under aerobic conditions without hydroxylamine as intermediate . Bioresour Technol , 277 : 87 - 93 . https://doi.org/10.1016/j.biortech.2019.01.040 https://doi.org/10.1016/j.biortech.2019.01.040

James SN , Vijayanandan A , 2023 . Recent advances in simultaneous nitrification and denitrification for nitrogen and micropollutant removal: a review . Biodegradation , 34 ( 2 ): 103 - 123 . https://doi.org/10.1007/s10532-023-10015-8 https://doi.org/10.1007/s10532-023-10015-8

Ke X , Liu C , Yu H , et al. , 2024 . Coculture of the heterotrophic nitrification-aerobic denitrification strains Acinetobacter sp. A12 and Paracoccus sp. T8 enables effective biological nitrogen removal from landfill leachate . ACS EST Water , 4 ( 4 ): 1786 - 1797 . https://doi.org/10.1021/acsestwater.3c00789 https://doi.org/10.1021/acsestwater.3c00789

Lei X , Jia YT , Chen YC , et al. , 2019 . Simultaneous nitrification and denitrification without nitrite accumulation by a novel isolated Ochrobactrum anthropic LJ81 . Bioresour Technol , 272 : 442 - 450 . https://doi.org/10.1016/j.biortech.2018.10.060 https://doi.org/10.1016/j.biortech.2018.10.060

Lei Y , Wang YQ , Liu HJ , et al. , 2016 . A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium . Appl Microbiol Biotechnol , 100 ( 9 ): 4219 - 4229 . https://doi.org/10.1007/s00253-016-7290-5 https://doi.org/10.1007/s00253-016-7290-5

Li SS , He ZM , Li C , et al. , 2024 . Nitrogen removal by heterotrophic nitrification-aerobic denitrification bacteria: a review . Desalin Water Treat , 317 : 100227 . https://doi.org/10.1016/j.dwt.2024.100227 https://doi.org/10.1016/j.dwt.2024.100227

Liao M , Luo YX , Xu N , et al. , 2022 . Nitrogen removal and metabolic pathway of Enterobacter cloacae DK-6 . Environ Technol Inno , 28 : 102630 . https://doi.org/10.1016/j.eti.2022.102630 https://doi.org/10.1016/j.eti.2022.102630

Liu TF , Wang BJ , Liu M , et al. , 2023 . Stutzerimonas frequens strain TF18 with superior heterotrophic nitrification-aerobic denitrification ability for the treatment of aquaculture effluent . Process Biochem , 130 : 156 - 165 . https://doi.org/10.1016/j.procbio.2023.04.018 https://doi.org/10.1016/j.procbio.2023.04.018

Liu WF , Wu YT , 2021 . Simultaneous nitrification, denitrification and electricity recovery of Halomonas strains in single chamber microbial fuel cells for seawater sewage treatment . J Environ Chem Eng , 9 ( 6 ): 106761 . https://doi.org/10.1016/j.jece.2021.106761 https://doi.org/10.1016/j.jece.2021.106761

Ma F , Sun YL , Li A , et al. , 2015 . Activation of accumulated nitrite reduction by immobilized Pseudomonas stutzeri T13 during aerobic denitrification . Bioresour Technol , 187 : 30 - 36 . https://doi.org/10.1016/j.biortech.2015.03.060 https://doi.org/10.1016/j.biortech.2015.03.060

Ma ML , Gui Q , Zheng WS , et al. , 2024 . Nitrogen removal mechanism and microbial community changes of the MBR bioaugmented with two novel fungi Pichia kudriavzevii N7 and Candida tropicalis N9 . Water , 16 ( 5 ): 757 . https://doi.org/10.3390/w16050757 https://doi.org/10.3390/w16050757

Ouyang L , Wang KJ , Liu XY , et al. , 2020 . A study on the nitrogen removal efficacy of bacterium Acinetobacter tandoii MZ-5 from a contaminated river of Shenzhen, Guangdong Province, China . Bioresour Technol , 315 : 123888 . https://doi.org/10.1016/j.biortech.2020.123888 https://doi.org/10.1016/j.biortech.2020.123888

Philippot L , Andert J , Jones CM , et al. , 2011 . Importance of denitrifiers lacking the genes encoding the nitrous oxide reductase for N 2 O emissions from soil . Glob Chang Biol , 17 ( 3 ): 1497 - 1504 . https://doi.org/10.1111/j.1365-2486.2010.02334.x https://doi.org/10.1111/j.1365-2486.2010.02334.x

Provolo G , Perazzolo F , Mattachini G , et al. , 2017 . Nitrogen removal from digested slurries using a simplified ammonia stripping technique . Waste Manage , 69 : 154 - 161 . https://doi.org/10.1016/j.wasman.2017.07.047 https://doi.org/10.1016/j.wasman.2017.07.047

Ren JL , Tang JJ , Min HP , et al. , 2024 . Nitrogen removal characteristics of novel bacterium Klebsiella sp. TSH15 by assimilatory/dissimilatory nitrate reduction and ammonia assimilation . Bioresour Technol , 394 : 130184 . https://doi.org/10.1016/j.biortech.2023.130184 https://doi.org/10.1016/j.biortech.2023.130184

Ren YX , Yang L , Liang X , 2014 . The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter junii YB . Bioresour Technol , 171 : 1 - 9 . https://doi.org/10.1016/j.biortech.2014.08.058 https://doi.org/10.1016/j.biortech.2014.08.058

Sanjrani MA , Nafees A , Sakina R , et al. , 2022 . A review on application of external carbon sources for denitrification for wastewater treatment . Global NEST J , 24 ( 1 ): 105 - 118 .

Silva LCF , Lima HS , de Oliveira Mendes TA , et al. , 2019 . Heterotrophic nitrifying/aerobic denitrifying bacteria: ammonium removal under different physical-chemical conditions and molecular characterization . J Environ Manage , 248 : 109294 . https://doi.org/10.1016/j.jenvman.2019.109294 https://doi.org/10.1016/j.jenvman.2019.109294

Wu SQ , LV N , Zhou Y , et al. , 2023 . Simultaneous nitrogen removal via heterotrophic nitrification and aerobic denitrification by a novel Lysinibacillus fusiformis B301 . Water Environ Res , 95 ( 3 ): e10850 . https://doi.org/10.1002/wer.10850 https://doi.org/10.1002/wer.10850

Xi HP , Zhou XT , Arslan M , et al. , 2022 . Heterotrophic nitrification and aerobic denitrification process: promising but a long way to go in the wastewater treatment . Sci Total Environ , 805 : 150212 . https://doi.org/10.1016/j.scitotenv.2021.150212 https://doi.org/10.1016/j.scitotenv.2021.150212

Xia L , Li XM , Fan WH , et al. , 2020 . Heterotrophic nitrification and aerobic denitrification by a novel Acinetobacter sp. ND7 isolated from municipal activated sludge . Bioresour Technol , 301 : 122749 . https://doi.org/10.1016/j.biortech.2020.122749 https://doi.org/10.1016/j.biortech.2020.122749

Xie FX , Thiri M , Wang H , 2021 . Simultaneous heterotrophic nitrification and aerobic denitrification by a novel isolated Pseudomonas mendocina X49 . Bioresour Technol , 319 : 124198 . https://doi.org/10.1016/j.biortech.2020.124198 https://doi.org/10.1016/j.biortech.2020.124198

Xu N , Liao M , Liang YQ , et al. , 2021 . Biological nitrogen removal capability and pathways analysis of a novel low C/N ratio heterotrophic nitrifying and aerobic denitrifying bacterium ( Bacillus thuringiensis strain WXN-23) . Environ Res , 195 : 110797 . https://doi.org/10.1016/j.envres.2021.110797 https://doi.org/10.1016/j.envres.2021.110797

Yang L , Wang XH , Cui S , et al. , 2019 . Simultaneous removal of nitrogen and phosphorous by heterotrophic nitrification-aerobic denitrification of a metal resistant bacterium Pseudomonas putida strain NP5 . Bioresour Technol , 285 : 121360 . https://doi.org/10.1016/j.biortech.2019.121360 https://doi.org/10.1016/j.biortech.2019.121360

Zhang MM , He TX , Chen MP , et al. , 2022 . Ammonium and hydroxylamine can be preferentially removed during simultaneous nitrification and denitrification by Pseudomonas taiwanensis EN-F2 . Bioresour Technol , 350 : 126912 . https://doi.org/10.1016/j.biortech.2022.126912 https://doi.org/10.1016/j.biortech.2022.126912

Zhang N , Chen H , Lyu Y , et al. , 2019 . Nitrogen removal by a metal-resistant bacterium, Pseudomonas putida ZN1, capable of heterotrophic nitrification-aerobic denitrification . J Chem Technol Biot , 94 ( 4 ): 1165 - 1175 . https://doi.org/10.1002/jctb.5863 https://doi.org/10.1002/jctb.5863

Zhang WW , Andong ZY , Zhang M , et al. , 2014 . Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in <math id="M89"><mi mathvariant="normal">N</mi><msubsup><mrow><mi mathvariant="normal">H</mi></mrow><mrow><mn mathvariant="normal">4</mn></mrow><mrow><mo>+</mo></mrow></msubsup></math> https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110857&type= https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=102110846&type= 5.75733376 3.47133350 -N removal . Ann Microbiol , 64 ( 3 ): 1231 - 1238 . https://doi.org/10.1007/s13213-013-0764-0 https://doi.org/10.1007/s13213-013-0764-0

Zhang YT , Yin SH , Li HY , et al. , 2022 . Treatment of ammonia-nitrogen wastewater by the ultrasonic strengthened break point chlorination method . J Water Process Eng , 45 : 102501 . https://doi.org/10.1016/j.jwpe.2021.102501 https://doi.org/10.1016/j.jwpe.2021.102501

Zhao B , He YL , Hughes J , et al. , 2010 . Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoaceticus HNR . Bioresour Technol , 101 ( 14 ): 5194 - 5200 . https://doi.org/10.1016/j.biortech.2010.02.043 https://doi.org/10.1016/j.biortech.2010.02.043

Zhao B , An Q , He YL , et al. , 2012 . N 2 O and N 2 production during heterotrophic nitrification by Alcaligenes faecalis strain NR . Bioresour Technol , 116 : 379 - 385 . https://doi.org/10.1016/j.biortech.2012.03.113 https://doi.org/10.1016/j.biortech.2012.03.113

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