泛凋亡（PANoptosis）及其相关基因在急性肝衰竭中的作用：通过生物信息学分析和动物实验验证所获得的新见解

葛田田; 陈瑶; 庞澜天; 邵骏威; 陈智

doi:10.1631/jzus.B2300678

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泛凋亡（PANoptosis）及其相关基因在急性肝衰竭中的作用：通过生物信息学分析和动物实验验证所获得的新见解

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Research Article | Updated：2025-04-23

泛凋亡（PANoptosis）及其相关基因在急性肝衰竭中的作用：通过生物信息学分析和动物实验验证所获得的新见解
Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2025年26卷第4期页码：353-370
Affiliations：

1.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
2.Department of Infectious Diseases, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
3.Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou 310009, China
Funds：

the National Science and Technology Major Project of China(2018ZX10302206)
DOI：10.1631/jzus.B2300678
中图分类号：
CSTR：
收稿日期：2023-09-19，

修回日期：2024-04-23，

纸质出版日期：2025-04-23
Accepted：

葛田田,陈瑶,庞澜天等.泛凋亡（PANoptosis）及其相关基因在急性肝衰竭中的作用：通过生物信息学分析和动物实验验证所获得的新见解[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(04):353-370.

Tiantian GE, Yao CHEN, Lantian PANG, et al. Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(4): 353-370.
葛田田,陈瑶,庞澜天等.泛凋亡（PANoptosis）及其相关基因在急性肝衰竭中的作用：通过生物信息学分析和动物实验验证所获得的新见解[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(04):353-370. DOI： 10.1631/jzus.B2300678.

Tiantian GE, Yao CHEN, Lantian PANG, et al. Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(4): 353-370. DOI： 10.1631/jzus.B2300678.

摘要

泛凋亡具有焦亡、凋亡和坏死性凋亡的特征。尽管大量研究已证实各类型细胞的死亡在急性肝衰竭（ALF）中发挥着不同作用，但对它们间的互作关注较少。本研究旨在探讨泛凋亡在ALF中的作用，并挖掘可作为预防或治疗ALF的新靶点。本研究首先从基因表达综合数据库（GEO）中下载三个与ALF相关的数据集（GSE14668、GSE62029和GSE74000），从中筛选差异表达基因（DEGs），并通过加权基因共表达网络分析（WGCNA）对上述基因和泛凋亡相关基因集取交集得到枢纽基因；通过基因本体论（GO）、京都基因与基因组百科全书（KEGG）、蛋白质-蛋白质互作（PPI）和基因集富集分析（GSEA）确定枢纽基因功能；最后利用ALF小鼠和细胞模型进行验证。结果表明，在ALF患者肝脏样本中，7个枢纽基因（

BMF

、

BNIP3L

、

CASP1

、

RIPK3

、

UACA

、

UNC5B

、

ZBP1

）表达水平均上调；在ALF小鼠模型中，BNIP3L、RIPK3、P-RIPK3、UACA和cleaved caspase-1表达上调，而CASP1和UNC5B的表达下调；ZBP-1和BMF的表达仅在造模过程中升高，终末期则无明显变化。小鼠肝组织免疫荧光显示，这7个枢纽基因在巨噬细胞中均有表达；western blot结果表明，在脂多糖（LPS）/

-氨基半乳糖（

-gal）引起的ALF小鼠模型中，焦亡、凋亡和坏死性凋亡均有发生；ALF细胞模型实验显示，骨髓源巨噬细胞在LPS刺激后形成泛凋亡小体。综上，巨噬细胞的泛凋亡有可能加速ALF的进展，此外本研究鉴定并验证的7个ALF枢纽基因将有希望作为未来深入研究ALF的诊断标志物或新的治疗靶点。

Abstract

Background

PANoptosis has the features of pyroptosis

apoptosis

and necroptosis. Numerous studies have confirmed the diverse roles of various types of cell death in acute liver failure (ALF)

but limited attention has been given to the crosstalk among them. In this study

we aimed to explore the role of PANoptosis in ALF and uncover new targets for its prevention or treatment.

Methods

Three ALF-related datasets (GSE14668

GSE62029

and GSE74000) were downloaded from the Gene Expression Omnibus (GEO) database to identify differentially expressed genes (DEGs). Hub genes were identified through intersecting DEGs

genes obtained from weighted gene co-expression network analysis (WGCNA)

and genes related to PANoptosis. Gene Ontology (GO)

Kyoto Encyclopedia of Genes and Genomes (KEGG)

protein‍‒‍protein interaction (PPI) analyses and gene set enrichment analysis (GSEA) were performed to determine functional roles. Verification was performed using an ALF mouse model.

Results

Our results showed that expression of seven hub genes (B-cell lymphoma-2-modifying factor (

BMF

)

B-cell lymphoma-2-interacting protein 3-like (

BNIP3L

)

Caspase-1 (

CASP1

)

receptor-interacting protein kinase 3 (

RIPK3

)

uveal autoantigen with coiled-coil domains and ankyrin repeats protein (

UACA

)

uncoordinated-5 homolog B receptor (

UNC5B

)

and Z-DNA-binding protein 1 (

ZBP1

)) was up-regulated in liver samples of patients. However

in the ALF mouse model

the expression of BNIP3L

RIPK3

phosphorylated RIPK3 (P-RIPK3)

UACA

and cleaved caspase-1 was up-regulated

while the expression of CASP1 and UNC5B was down-regulated. The expression of ZBP1 and BMF increased only during the development of ALF

and there was no significant change in the end stage. Immunofluorescence of mouse liver tissue showed that macrophages

expressed all seven markers. Western blot results showed that pyroptosis

apoptosis

and necroptosis were always involved in lipopolysaccharide (LPS)/

-galactosamine (

-gal)‍-induced ALF mice. The ALF cell model showed that bone marrow-derived macrophages (BMDMs) form PANoptosomes after LPS stimulation.

Conclusions

Our results suggest that PANoptosis of macrophages promotes the development of ALF. The seven new ALF biomarkers identified and validated in this study may contribute to further investigation of diagnostic markers or novel therapeutic targets of ALF.

关键词

Keywords

references

Bernal W , Wendon J , 2013 . Acute liver failure . N Engl J Med , 369 ( 26 ): 2525 - 2534 . https://doi.org/10.1056/NEJMra1208937 https://doi.org/10.1056/NEJMra1208937

Chen YJ , Jiao DY , Liu Y , et al. , 2023 . FBXL4 mutations cause excessive mitophagy via BNIP3/BNIP3L accumulation leading to mitochondrial DNA depletion syndrome . Cell Death Differ , 30 ( 10 ): 2351 - 2363 . https://doi.org/10.1038/s41418-023-01205-1 https://doi.org/10.1038/s41418-023-01205-1

Choi SW , Oh H , Park SY , et al. , 2022 . Netrin-1 attenuates hepatic steatosis via UNC5b/PPARγ‍-‍mediated suppression of inflammation and ER stress . Life Sci , 311 : 121149 . https://doi.org/10.1016/j.lfs.2022.121149 https://doi.org/10.1016/j.lfs.2022.121149

Christgen S , Zheng M , Kesavardhana S , et al. , 2020 . Identification of the panoptosome: a molecular platform triggering pyroptosis, apoptosis, and necroptosis (panoptosis) . Front Cell Infect Microbiol , 10 : 237 . https://doi.org/10.3389/fcimb.2020.00237 https://doi.org/10.3389/fcimb.2020.00237

Duan YW , Chen SX , Li QY , et al. , 2022 . Neuroimmune mechanisms underlying neuropathic pain: the potential role of TNF-α-necroptosis pathway . Int J Mol Sci , 23 ( 13 ): 7191 . https://doi.org/10.3390/ijms23137191 https://doi.org/10.3390/ijms23137191

Fang YX , Ma KY , Huang YM , et al. , 2023 . Fibronectin leucine-rich transmembrane protein 2 drives monocyte differentiation into macrophages via the UNC5B-Akt/mTOR axis . Front Immunol , 14 : 1162004 . https://doi.org/10.3389/fimmu.2023.1162004 https://doi.org/10.3389/fimmu.2023.1162004

Galluzzi L , Kepp O , Krautwald S , et al. , 2014 . Molecular mechanisms of regulated necrosis . Semin Cell Dev Biol , 35 : 24 - 32 . https://doi.org/10.1016/j.semcdb.2014.02.006 https://doi.org/10.1016/j.semcdb.2014.02.006

Galluzzi L , Bravo-San Pedro JM , Vitale I , et al. , 2015 . Essential versus accessory aspects of cell death: recommendations of the NCCD 2015 . Cell Death Differ , 22 ( 1 ): 58 - 73 . https://doi.org/10.1038/cdd.2014.137 https://doi.org/10.1038/cdd.2014.137

Green DR , Kroemer G , 2005 . Pharmacological manipulation of cell death: clinical applications in sight? J Clin Invest , 115 ( 10 ): 2610 - 2617 . https://doi.org/10.1172/JCI26321 https://doi.org/10.1172/JCI26321

Guillot A , Tacke F , 2023 . Spatial dimension of macrophage heterogeneity in liver diseases . eGastroenterology , 1 ( 1 ): e000003 . https://doi.org/10.1136/egastro-2023-000003 https://doi.org/10.1136/egastro-2023-000003

Hao Y , Yang B , Yang JK , et al. , 2022 . ZBP1: a powerful innate immune sensor and double-edged sword in host immunity . Int J Mol Sci , 23 ( 18 ): 10224 . https://doi.org/10.3390/ijms231810224 https://doi.org/10.3390/ijms231810224

Huang L , An XZ , Zhu Y , et al. , 2022 . Netrin-1 induces the anti-apoptotic and pro-survival effects of B-ALL cells through the Unc5b-MAPK axis . Cell Commun Signal , 20 : 122 . https://doi.org/10.1186/s12964-022-00935-y https://doi.org/10.1186/s12964-022-00935-y

Huo CY , Tang YL , Li XS , et al. , 2023 . Melatonin alleviates lung injury in H1N1-infected mice by mast cell inactivation and cytokine storm suppression . PLoS Pathog , 19 ( 5 ): e1011406 . https://doi.org/10.1371/journal.ppat.1011406 https://doi.org/10.1371/journal.ppat.1011406

Jiang WK , Sun SK , Wang DY , et al. , 2022 . MicroRNA-22 suppresses NLRP3/CASP1 inflammasome pathway-mediated proinflammatory cytokine production by targeting the HIF-1α and NLRP3 in human dental pulp fibroblasts . Int Endod J , 55 ( 11 ): 1225 - 1240 . https://doi.org/10.1111/iej.13814 https://doi.org/10.1111/iej.13814

Karki R , Sundaram B , Sharma BR , et al. , 2021a . ADAR1 restricts ZBP1-mediated immune response and PANoptosis to promote tumorigenesis . Cell Rep , 37 ( 3 ): 109858 . https://doi.org/10.1016/j.celrep.2021.109858 https://doi.org/10.1016/j.celrep.2021.109858

Karki R , Sharma BR , Tuladhar S , et al. , 2021b . Synergism of TNF‍-‍α and IFN-‍γ triggers inflammatory cell death, tissue damage, and mortality in SARS-CoV-2 infection and cytokine shock syndromes . Cell , 184 ( 1 ): 149 - 168.e17 . https://doi.org/10.1016/j.cell.2020.11.025 https://doi.org/10.1016/j.cell.2020.11.025

Khan SA , Shah N , Williams R , et al. , 2006 . Acute liver failure: a review . Clin Liver Dis , 10 ( 2 ): 239 - 258 . https://doi.org/10.1016/j.cld.2006.05.010 https://doi.org/10.1016/j.cld.2006.05.010

Koch AW , Mathivet T , Larrivée B , et al. , 2011 . Robo4 maintains vessel integrity and inhibits angiogenesis by interacting with UNC5B . Dev Cell , 20 ( 1 ): 33 - 46 . https://doi.org/10.1016/j.devcel.2010.12.001 https://doi.org/10.1016/j.devcel.2010.12.001

Kuriakose T , Man SM , Subbarao Malireddi RK , et al. , 2016 . ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways . Sci Immunol , 1 ( 2 ): aag2045 . https://doi.org/10.1126/sciimmunol.aag2045 https://doi.org/10.1126/sciimmunol.aag2045

Leek JT , Johnson WE , Parker HS , et al. , 2012 . The sva package for removing batch effects and other unwanted variation in high-throughput experiments . Bioinformatics , 28 ( 6 ): 882 - 883 . https://doi.org/10.1093/bioinformatics/bts034 https://doi.org/10.1093/bioinformatics/bts034

Li AC , Yang Q , Lou GH , et al. , 2021 . 5-‍((7-Chloro-6-fluoro-1h-indol-3-yl) methyl)‍-3-methylimidazolidine-2,‍4-dione as a RIP1 inhibitor protects LPS/ d -galactosamine-induced liver failure . Life Sci , 273 : 119304 . https://doi.org/10.1016/j.lfs.2021.119304 https://doi.org/10.1016/j.lfs.2021.119304

Li XX , Li XC , Yang JL , et al. , 2023 . In situ sustained macrophage-targeted nanomicelle-hydrogel microspheres for inhibiting osteoarthritis . Research (Wash D C) , 6 : 0131 . https://doi.org/10.34133/research.0131 https://doi.org/10.34133/research.0131

Lin JF , Hu PS , Wang YY , et al. , 2022 . Phosphorylated NFS1 weakens oxaliplatin-based chemosensitivity of colorectal cancer by preventing panoptosis . Signal Transduct Target Ther , 7 : 54 . https://doi.org/10.1038/s41392-022-00889-0 https://doi.org/10.1038/s41392-022-00889-0

Liu L , Sakai T , Tran NH , et al. , 2009 . Nucling interacts with nuclear factor-‍κB, regulating its cellular distribution . FEBS J , 276 ( 5 ): 1459 - 1470 . https://doi.org/10.1111/j.1742-4658.2009.06888.x https://doi.org/10.1111/j.1742-4658.2009.06888.x

Long JS , Ryan KM , 2012 . New frontiers in promoting tumour cell death: targeting apoptosis, necroptosis and autophagy . Oncogene , 31 ( 49 ): 5045 - 5060 . https://doi.org/10.1038/onc.2012.7 https://doi.org/10.1038/onc.2012.7

Malireddi RKS , Gurung P , Mavuluri J , et al. , 2018 . TAK1 restricts spontaneous NLRP3 activation and cell death to control myeloid proliferation . J Exp Med , 215 ( 4 ):‍ 1023 - 1034 . https://doi.org/10.1084/jem.20171922 https://doi.org/10.1084/jem.20171922

Malireddi RKS , Kesavardhana S , Kanneganti TD , 2019 . ZBP1 and TAK1: master regulators of NLRP3 inflammasome/pyroptosis, apoptosis, and necroptosis (PAN-optosis) . Front Cell Infect Microbiol , 9 : 406 . https://doi.org/10.3389/fcimb.2019.00406 https://doi.org/10.3389/fcimb.2019.00406

Newton K , 2015 . RIPK1 and RIPK3: critical regulators of inflammation and cell death . Trends Cell Biol , 25 ( 6 ): 347 - 353 . https://doi.org/10.1016/j.tcb.2015.01.001 https://doi.org/10.1016/j.tcb.2015.01.001

Nguyen LN , Kanneganti TD , 2022 . PANoptosis in viral infection: the missing puzzle piece in the cell death field . J Mol Biol , 434 ( 4 ): 167249 . https://doi.org/10.1016/j.jmb.2021.167249 https://doi.org/10.1016/j.jmb.2021.167249

Ohkura T , Taniguchi SI , Yamada K , et al. , 2004 . Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves’ disease . Biochem Biophys Res Commun , 321 ( 2 ): 432 - 440 . https://doi.org/10.1016/j.bbrc.2004.06.162 https://doi.org/10.1016/j.bbrc.2004.06.162

Pan HD , Pan JX , Li P , et al. , 2022 . Characterization of PANoptosis patterns predicts survival and immunotherapy response in gastric cancer . Clin Immunol , 238 : 109019 . https://doi.org/10.1016/j.clim.2022.109019 https://doi.org/10.1016/j.clim.2022.109019

Petrik J , 2006 . Diagnostic applications of microarrays . Transfus Med , 16 ( 4 ): 233 - 247 . https://doi.org/10.1111/j.1365-3148.2006.00673.x https://doi.org/10.1111/j.1365-3148.2006.00673.x

Puthalakath H , Strasser A , 2002 . Keeping killers on a tight leash: transcriptional and post-translational control of the pro-apoptotic activity of BH3-only proteins . Cell Death Differ , 9 ( 5 ): 505 - 512 . https://doi.org/10.1038/sj.cdd.4400998 https://doi.org/10.1038/sj.cdd.4400998

Puthalakath H , Villunger A , O'Reilly LA , et al. , 2001 . Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis . Science , 293 ( 5536 ): 1829 - 1832 . https://doi.org/10.1126/science.1062257 https://doi.org/10.1126/science.1062257

Ramjaun AR , Tomlinson S , Eddaoudi A , et al. , 2007 . Upregulation of two BH3-only proteins, Bmf and Bim, during TGF β -induced apoptosis . Oncogene , 26 ( 7 ): 970 - 981 . https://doi.org/10.1038/sj.onc.1209852 https://doi.org/10.1038/sj.onc.1209852

Rebsamen M , Heinz LX , Meylan E , et al. , 2009 . DAI/ZBP1 recruits RIP1 and RIP3 through RIP homotypic interaction motifs to activate NF-κB . EMBO Rep , 10 ( 8 ): 916 - 922 . https://doi.org/10.1038/embor.2009.109 https://doi.org/10.1038/embor.2009.109

Sakai T , Liu L , Shishido Y , et al. , 2003 . Identification of a novel, embryonal carcinoma cell-associated molecule, nucling, that is up-regulated during cardiac muscle differentiation . J Biochem , 133 ( 4 ): 429 - 436 . https://doi.org/10.1093/jb/mvg056 https://doi.org/10.1093/jb/mvg056

Schwabe RF , Luedde T , 2018 . Apoptosis and necroptosis in the liver: a matter of life and death . Nat Rev Gastroenterol Hepatol , 15 ( 12 ): 738 - 752 . https://doi.org/10.1038/s41575-018-0065-y https://doi.org/10.1038/s41575-018-0065-y

Slowik A , Lammerding L , Zendedel A , et al. , 2018 . Impact of steroid hormones E2 and P on the NLRP3/ASC/Casp1 axis in primary mouse astroglia and BV-2 cells after in vitro hypoxia . J Steroid Biochem Mol Biol , 183 : 18 - 26 . https://doi.org/10.1016/j.jsbmb.2018.05.003 https://doi.org/10.1016/j.jsbmb.2018.05.003

Sowter HM , Ratcliffe PJ , Watson P , et al. , 2001 . HIF-1-dependent regulation of hypoxic induction of the cell death factors BNIP3 and NIX in human tumors . Cancer Res , 61 ( 18 ): 6669 - 6673 .

Speir M , Lawlor KE , 2021 . RIP-roaring inflammation: RIPK1 and RIPK3 driven NLRP3 inflammasome activation and autoinflammatory disease . Semin Cell Dev Biol , 109 : 114 - 124 . https://doi.org/10.1016/j.semcdb.2020.07.011 https://doi.org/10.1016/j.semcdb.2020.07.011

Stravitz RT , Lee WM , 2019 . Acute liver failure . Lancet , 394 ( 10201 ): 869 - 881 . https://doi.org/10.1016/S0140-6736(19)31894-X https://doi.org/10.1016/S0140-6736(19)31894-X

Su ZQ , Ning BT , Fang H , et al. , 2011 . Next-generation sequencing and its applications in molecular diagnostics . Expert Rev Mol Diagn , 11 ( 3 ): 333 - 343 . https://doi.org/10.1586/erm.11.3 https://doi.org/10.1586/erm.11.3

Takaoka A , Wang ZC , Choi MK , et al. , 2007 . DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response . Nature , 448 ( 7152 ): 501 - 505 . https://doi.org/10.1038/nature06013 https://doi.org/10.1038/nature06013

Vasudevan SO , Behl B , Rathinam VA , 2023 . Pyroptosis-induced inflammation and tissue damage . Semin Immunol , 69 : 101781 . https://doi.org/10.1016/j.smim.2023.101781 https://doi.org/10.1016/j.smim.2023.101781

Verbeke J , de Bolle X , Arnould T , 2023 . When mitophagy dictates the outcome of cellular infection: the case of Brucella abortus . Autophagy , 19 ( 11 ): 3022 - 3023 . https://doi.org/10.1080/15548627.2023.2246354 https://doi.org/10.1080/15548627.2023.2246354

Wang DY , Zhang JB , Jiang WK , et al. , 2017 . The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability . Autophagy , 13 ( 5 ): 914 - 927 . https://doi.org/10.1080/15548627.2017.1293766 https://doi.org/10.1080/15548627.2017.1293766

Wang Y , Chen Q , Jiao FZ , et al. , 2021 . Histone deacetylase 2 regulates ULK1 mediated pyroptosis during acute liver failure by the K68 acetylation site . Cell Death Dis , 12 : 55 . https://doi.org/10.1038/s41419-020-03317-9 https://doi.org/10.1038/s41419-020-03317-9

Yamamura Y , Lee WL , Inoue K , et al. , 2006 . RUNX3 cooperates with FoxO3a to induce apoptosis in gastric cancer cells . J Biol Chem , 281 ( 8 ): 5267 - 5276 . https://doi.org/10.1074/jbc.M512151200 https://doi.org/10.1074/jbc.M512151200

Yan WT , Zhao WJ , Hu XM , et al. , 2023 . PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons . Neural Regen Res , 18 ( 2 ): 357 - 363 . https://doi.org/10.4103/1673-5374.346545 https://doi.org/10.4103/1673-5374.346545

Yang X , Ma LM , Zhang J , et al. , 2023 . Hypofucosylation of Unc5b regulated by Fut8 enhances macrophage emigration and prevents atherosclerosis . Cell Biosci , 13 : 13 . https://doi.org/10.1186/s13578-023-00959-y https://doi.org/10.1186/s13578-023-00959-y

Yu XB , Liu X , Liu X , et al. , 2023 . Overexpression of CASP1 triggers acute promyelocytic leukemia cell pyroptosis and differentiation . Eur J Pharmacol , 945 : 175614 . https://doi.org/10.1016/j.ejphar.2023.175614 https://doi.org/10.1016/j.ejphar.2023.175614

Zheng YF , Sun WR , Shan C , et al. , 2022 . β‍-‍Hydroxybutyrate inhibits ferroptosis-mediated pancreatic damage in acute liver failure through the increase of H3K9bhb . Cell Rep , 41 ( 12 ): 111847 . https://doi.org/10.1016/j.celrep.2022.111847 https://doi.org/10.1016/j.celrep.2022.111847

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