床旁即时检测分析仪在血清生化十三项指标与全血凝血五项指标的应用：评估呼吸与危重症疾病的理想工具

刘铭涛; 刘俐; 陈珈晞; 黄志锋; 朱惠清; 林盛铉; 戚维天; 程章恺; 李宁; 孙宝清

doi:10.1631/jzus.B2300433

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床旁即时检测分析仪在血清生化十三项指标与全血凝血五项指标的应用：评估呼吸与危重症疾病的理想工具

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Research Article | Updated：2025-02-25

床旁即时检测分析仪在血清生化十三项指标与全血凝血五项指标的应用：评估呼吸与危重症疾病的理想工具
Thirteen serum biochemical indexes and five whole blood coagulation indices in a point-of-care testing analyzer: ideal protocol for evaluating pulmonary and critical care medicine
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2025年26卷第2期页码：158-171
Affiliations：

1.Department of Clinical Laboratory, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, Guangzhou Laboratory, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510140, China
2.KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou 511495, China
3.College of Medical Technology, Guangdong Medical University, Dongguan 523121, China
4.Second Clinical Medical School, Southern Medical University, Guangzhou 510515, China
Funds：

the National Natural Science Foundation of China(82302607);the Guangdong Basic and Applied Basic Research Foundation(2022A1515110555;2023A1515010932);the State Key Laboratory of Respiratory Disease(SKLRD-Z-202410);the Precision Medicine Joint Foundation of Basic and Applied Basic Research Foundation of Guangdong Province(2021B1515230008);the Independent Project of Guangdong-Hong Kong-Macao Joint Laboratory for Respiratory Infectious Diseases(GHMJLRID-Z-202102);the Guangdong Zhong Nanshan Medical Foundation(ZNSXS-20220015)
DOI：10.1631/jzus.B2300433
中图分类号：
CSTR：
收稿日期：2023-06-12，

修回日期：2023-11-08，

网络出版日期：2025-01-18，

纸质出版日期：2025-02-25
Accepted：

刘铭涛,刘俐,陈珈晞等.床旁即时检测分析仪在血清生化十三项指标与全血凝血五项指标的应用：评估呼吸与危重症疾病的理想工具[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(02):158-171.

Mingtao LIU, Li LIU, Jiaxi CHEN, et al. Thirteen serum biochemical indexes and five whole blood coagulation indices in a point-of-care testing analyzer: ideal protocol for evaluating pulmonary and critical care medicine[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(2): 158-171.
刘铭涛,刘俐,陈珈晞等.床旁即时检测分析仪在血清生化十三项指标与全血凝血五项指标的应用：评估呼吸与危重症疾病的理想工具[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(02):158-171. DOI： 10.1631/jzus.B2300433.

Mingtao LIU, Li LIU, Jiaxi CHEN, et al. Thirteen serum biochemical indexes and five whole blood coagulation indices in a point-of-care testing analyzer: ideal protocol for evaluating pulmonary and critical care medicine[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(2): 158-171. DOI： 10.1631/jzus.B2300433.

摘要

生化和凝血指标的准确及时检测对呼吸及危重症患者至关重要。传统实验室检测虽可靠，但往往较为滞后。床边即时检测（POCT）已成为一种潜在的替代方法，但仍待严格验证。使用Beckman Coulter AU5821和PUSHKANG POCT生化分析仪以及Stago STAR MAX和PUSHKANG POCT凝血分析仪分别对广州医科大学附属第一医院的226份血清和350份全血样本进行包括白蛋白等十三项生化指标和凝血酶原时间等五项凝血指标进行比对检测。Bland-Altman图（MS100: 0.8206‒0.9995; MC100: 0.8318‒0.9911）一致性较好。Spearman相关分析显示，传统仪器和PUSHKANG POCT分析仪之间存在显著的线性相关性，且相关系数稳健（MS100: 0.713‒0.949; MC100: 0.593‒0.950）。PUSHKANG POCT作为一种可靠的血清、全血生化和凝血快速诊断工具，具有稳定的临床评价功能，可为呼吸与危重症临床医师在患者紧急病况时提供及时预示。

Abstract

The accurate and timely detection of biochemical coagulation indicators is pivotal in pulmonary and critical care medicine. Despite their reliability

traditional laboratories often lag in terms of rapid diagnosis. Point-of-care testing (POCT) has emerged as a promising alternative

which is awaiting rigorous validation. We assessed 226 samples from patients at the First Affiliated Hospital of Guangzhou Medical University using a Beckman Coulter AU5821 and a PUSHKANG POCT Biochemistry Analyzer MS100. Furthermore

350 samples were evaluated with a Stago coagulation analyzer STAR MAX and a PUSHKANG POCT Coagulation Analyzer MC100. Metrics included thirteen biochemical indexes

such as albumin

and five coagulation indices

such as prothrombin time. Comparisons were drawn against the PUSHKANG POCT analyzer. Bland-Altman plots (MS100: 0.8206‒0.9995; MC100: 0.8318‒0.9911) evinced significant consistency between methodologies. Spearman correlation pinpointed a potent linear association between conventional devices and the PUSHKANG POCT analyzer

further underscored by a robust correlation coefficient (MS100: 0.713‒0.949; MC100: 0.593‒0.950). The PUSHKANG POCT was validated as a dependable tool for serum and whole blood biochemical and coagulation diagnostics. This emphasizes its prospective clinical efficacy

offering clinicians a swift diagnostic tool and heralding a new era of enhanced patient care outcomes.

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