Antibacterial potency of methanol extracts of lower plants

Antibacterial potency of methanol extracts of lower plants first-author †E-mail:walelugba@yahoo.com †E-mail:walelugba@yahoo.com walelugba@yahoo.com Department of Microbiology, Faculty of Science, University of Ado-Ekiti, Ado-Ekiti, Ekiti State 234030, Nigeria Department of Microbiology, Faculty of Science, University of Ado-Ekiti, Ado-Ekiti, Ekiti State 234030, Nigeria Antibacterial potency of methanol extracts of three green lower plants, Pneumatopteris afra , Platycerium bifurcatum and Nephrolepsis bisserata was determined using agar dilution method on clinical strains of Escherichia coli , Staphylococcus aureus , Klebsiella spp. and Salmomelia typhi . Antibacterial activities were observed at concentrations of 12.5, 25.0, 50.0 and 100.0 µg/ml. Their minimum inhibitory concentrations ranged from 12.5~100 μg/ml. Extracts of P . afra and P . bifurcatum were most active. Antibacterial activities observed with N . bisserata were less pronounced with no detectable activity at extract concentrations of 12.5 and 25.0 µg/ml. E . coli , together with S . aureus appeared to be the most susceptible of the test bacteria while Klebsiella spp. was least sensitive. The significance of our findings is discussed. Lower plants Extracts Antibacterial activity MIC (minimum inhibitory concentration) INTRODUCTION 1 In recent times, the search for potent antibacterial agents has been shifted to plants. However, the major part of the search has focused mainly on higher plants (Oyagade, 1998 1998 ; Olukoya et al., 2003 2003 ) while little attention is given to lower plants with possible antimicrobial properties. Most plants are medicinally useful in treating disease in the body and in most cases, the antimicrobial efficacy value attributed to some plants is beyond belief. Conservative estimates suggest that about 10% of all flowering plants on earth have at one time, been used by local communities throughout the world but only 1% have gained recognition by modern scientists (Kafaru, 1994 1994 ). Liverwort, mosses and hornwort are small, low-growing plants, which constitute the phylum bryophyta. They lack true stems, leaves and roots and modify their microclimate by conserving moisture, checking soil erosion on lilly slopes and serve as seedbed for forest cover. They are now increasingly used for diverse purposes including pollution control and as new sources of pharmaceuticals (Saxena and Saxena, 2002 2002 ). Liverworts and mosses have been tested and used as fuel in developed countries like Finland, Sweden, Ireland, Germany, Poland and the Soviet Union (Baker and Hawkinson, 1993 1993 ). Chinese traditional medicine named 40 kinds of bryophytes used to treat cardiovascular diseases, tonsillitis, bronchitis, cystitis and skin infections. It has also been shown that an extract of Giganteum can increase aorta blood transport by up to 30% in animals (Jorgnersteen, 1998 1998 ). The aim of this study is to determine the antimicrobial potencies of three lower plants with a view to ascertaining their possible medicinal values. MATERIALS AND METHODS 1 Plant material 2 Whole parts of Pneumatopteris afra , Platycerium bifurcatum and Nephrolepsis bisserata were collected in Ado-Ekiti, Ekiti State, Nigeria. Identification of plants was done at the herbarium unit, Department of Plant Science, University of Ado-Ekiti, Nigeria. Voucher specimens are deposited in this unit. Preparation of extracts 2 In each case, powdered air-dried plant material was extracted with methanol. The crude methanol extract was prepared by maceration of plant material (100 g) with methanol (300 ml) (Alanis et al., 2005 2005 ). The extract was filtered and evaporated to dryness in vacuum. Bacterial strains 2 All bacterial strains used in this study were clinical strains. They are Escherichia coli , Staphylococcus aureus , Klebsiella spp. and Salmonella typhi . Antibacterial testing 2 Antibacterial activity was measured using agar dilution technique. Briefly, the methanol extracts were dissolved in dimethyl sulfoxide (DMSO, Merck) and serially diluted in molten Mueller Hinton agar (MHA, Sigma) in petridishes (100 mm×15 mm) to obtain final concentrations: 100, 50, 25 and 12.5 µg/ml. The solvent did not exceed 1% concentration and did not affect the growth of the organisms. All bacterial strains were grown in Mueller Hinton broth (MHB, Sigma) for 4 h at 37 °C. Bacterial suspensions with 0.5 McFarland standard turbidity, which is equivalent to 108 cfu/ml, were prepared by dilution with Mueller Hinton broth. The diluted inoculum was added to a Steer’s replicator calibrated and incubated for 24 h at 37 °C. After incubation, all dishes were observed for microbial inhibition. The minimum inhibitory concentration (MIC) was determined as the lowest concentration that completely inhibited macroscopic growth of the organisms. RESULTS 1 The results obtained in this study showed that the organisms were susceptible to the methanol extracts of the lower plants based on their minimum inhibitory concentrations. In general, the organisms showed the same susceptibility to methanol extracts of P . afra and P . bifurcatum with minimum inhibitory concentrations ranging from 12.50~100 μg/ml (Table 1 1 ); while they were least sensitive to N . bisserata (Table 1 1 ). E . coli showed the highest sensitivity to methanol extracts of P . afra and P . bifurcatum , followed by S . aureus while Klebsiella spp. are the most insensitive of all the organisms tested. E . coli also showed the highest sensitivity to N . bisserata . Salmonella typhi did not show any sensitivity to all the concentrations of N . bisserata and none of the organisms tested showed sensitivity to N . bisserata at concentrations of 25 µg/ml and 12.5 µg/ml. P . afra P . bifurcatum N . bisserata E . coli 12.50 12.50 50.00 S . aureus 12.50 12.50 50.00 Klebsiella spp. 100.00 100.00 50.00 S . typhi 25.00 25.00 100.00 DISCUSSION 1 Due to the increasing prevalence of antibiotic-resistant pathogens in hospital and homes, deliberate search is in progress for alternative treatments to combat further spread of antibiotic resistant-pathogens (Olukoya et al., 2003 2003 ). Different parts of higher plants have been screened for their potential antimicrobial properties but studies on the antimicrobial properties and activities of lower plants are very scanty (Oyagade, 1998 1998 ; Sofowora, 1998 1998 ). This study has, however, revealed that lower plants also possess antimicrobial properties. The overall results showed that the organisms were susceptible to different concentrations of methanol extracts of the lower plants, which is a function of their antimicrobial property. This is consistent with other works (Oyagade, 1998 1998 ; Jorgnersteen, 1998 1998 ; Smith and Reynard, 1992 1992 ). The antibacterial properties of the lower plants observed in this study could be attributed to chemicals including polygodial, norpiquisone and lunularin, all of which constitute the phytochemical components of lower plants (Smith and Reynard, 1992 1992 ). P . afra and P . bifurcatum were more active than N . bisserata as observed by their minimum inhibitory concentrations (12.5~100 µg/ml). Both plants showed exactly the same minimum inhibitory concentrations for the individual organisms. This implies that P . afra and P . bifurcatum could be better options for use as alternative treatment for infections that could be caused by any of these organisms. No activity was detected for N . bisserata at 12.5 µg/ml and 25.0 µg/ml for all the organisms tested (Table 1 1 ). The different susceptibilities of the individual organisms to different concentrations of extracts were noted. It was observed that E . coli and S . aureus were most sensitive to methanol extract of P . afra and P . bifurcatum at all concentrations as evident in the enhanced minimum inhibitory concentrations against these organisms (12.5~100 µg/ml). S . typhi follows immediately. Klebsiella spp. was most insensitive of all the test organisms. This study also showed that S . typhi was least sensitive to N . bisserata ( MIC =50μg/ml). The variation in the susceptibilities of these organisms could be attributed to the intrinsic properties of these organisms, which related to the permeability of their cell surface to the extracts. Further studies are needed to look into this aspect. Finally, this study revealed that apart from higher plants, the lower plants also possess some antibacterial properties, which make them candidates for alternative drugs in the not too distant future. References AD Alanis , F Glazada , JA Cervantes , J Tarres , GM Ceballas . Antibacterial properties of some plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders . J Ethnopharmacol , 2005 . 100 ( 1-2 ): 153 - 157 . 16005589 DOI: 10.1016/j.jep.2005.02.022 http://doi.org/10.1016/j.jep.2005.02.022 . Antibacterial properties of some plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders CN Baker , RW Hawkinson . Inoculum standardization in antimicrobial susceptibility test evaluation of the overnight aquaculture and rapid innoculum standarization system . J Clin Microbiol World , 1993 . 17 451 - 457 . . 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