Development and application of a novel TaqMan qPCR assay targeting the <italic style="font-style: italic">gN</italic> gene for genotyping pseudorabies virus

Yulan JIN; Xuan JI; Weiren DONG; Yanming HUANG; Jiyong ZHOU; Jinyan GU

doi:10.1631/jzus.B2500729

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Correspondence | Updated：2026-08-13

Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus
Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus
基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用
Journal of Zhejiang University-SCIENCE B Vol. 27, Issue 8, Pages: 923-926(2026)
Affiliations：

1.The Experimental Teaching Center, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
2.MOA Key Laboratory of Animal Virology, Zhejiang University Center for Veterinary Sciences, Hangzhou 310058, China
Funds：
DOI：10.1631/jzus.B2500729
CLC：
CSTR：
Received：13 November 2025，

Revised：2026-02-24，

Online First：13 August 2026，

Published：13 August 2026
Accepted：

金玉兰,季暄,董伟仁等.基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(08):923-926.

Yulan JIN, Xuan JI, Weiren DONG, et al. Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(8): 923-926. DOI: 10.1631/jzus.B2500729.
金玉兰,季暄,董伟仁等.基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2026,27(08):923-926. DOI： 10.1631/jzus.B2500729.

Yulan JIN, Xuan JI, Weiren DONG, et al. Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus[J]. Journal of Zhejiang University-SCIENCE B, 2026, 27(8): 923-926. DOI: 10.1631/jzus.B2500729. DOI：

摘要

伪狂犬病是由伪狂犬病病毒（PRV）引起的一种严重危害全球养猪业的传染病，由于不同基因型毒株的致病性与流行特征存在差异，临床上需建立一种能够快速、准确区分PRV基因I型和基因II型的分型检测方法。本研究首次以

基因为靶标，基于其保守区域鉴别稳定差异序列，成功建立了一种TaqMan三重实时荧光定量聚合酶链式反应（PCR）检测方法。该方法不仅可检测PRV感染，而且能有效区分其基因型。该检测方法具有特异性强、灵敏度高、重复性好及操作简便等优点。该检测体系仅需一对引物和三个探针即可完成分型鉴定，极大简化了反应体系，降低了引物错配及非特异性扩增的风险。对实验动物来源样本初步应用的结果显示，该方法能准确检测PRV的感染情况及其基因型。本研究为PRV的分子流行病学监测提供了实用、高效的技术工具，也为猪群中PRV的净化工作提供了有力支持。

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Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus

Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus

DOI：10.1631/jzus.B2500729

摘要

Abstract

关键词

Keywords

references