Functional redundancy of three mitochondrial Mg<sup>2+</sup>/Mn<sup>2+</sup>-dependent protein phosphatases (PPMs) in <italic style="font-style: italic">Toxoplasma gondii</italic>

Kaiyin SHENG; Xueqiu CHEN; Yimin YANG; Jie XIA; Kaiyue SONG; Chaoqun YAO; Yi YANG; Aifang DU; Guangxu MA

doi:10.1631/jzus.B2400308

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Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii

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Correspondence | Updated：2025-04-23

Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii
Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii
刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Vol. 26, Issue 4, Pages: 405-408(2025)
Affiliations：

1.College of Animal Sciences, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China
2.ZJU-Xinchang Joint Innovation Centre (Tianmu Laboratory), Gaochuang Hi-Tech Park, Xinchang 312500, China
3.Department of Biomedical Sciences and One Health Center for Zoonoses and Tropical Infectious Diseases, Ross University School of Veterinary Medicine, Basseterre KN-0101, The Federation of Saint Kitts and Nevis
Funds：
DOI：10.1631/jzus.B2400308
CLC：
CSTR：
Received：18 June 2024，

Revised：06 September 2024，

Published：23 April 2025
Accepted：

盛楷茵,陈学秋,阳毅敏等.刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(04):405-408.

Kaiyin SHENG, Xueqiu CHEN, Yimin YANG, et al. Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(4): 405-408.
盛楷茵,陈学秋,阳毅敏等.刚地弓形虫三种线粒体Mg²⁺/Mn²⁺依赖性蛋白磷酸酶（PPMs）存在功能冗余[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(04):405-408. DOI： 10.1631/jzus.B2400308.

Kaiyin SHENG, Xueqiu CHEN, Yimin YANG, et al. Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(4): 405-408. DOI： 10.1631/jzus.B2400308.

摘要

Abstract

弓形虫（

Toxoplasma gondii

）是一种专性胞内寄生的顶复门原虫，可以感染包括人在内的几乎所有温血动物，引起全球性的人畜共患弓形虫病，从而危害人类健康和畜牧业发展，并造成经济损失。刚地弓形虫独特的线粒体是弓形虫病防控的理想靶细胞器。线粒体中的Mg

/Mn

依赖性蛋白磷酸酶（PPMs）在细胞生长、能量代谢和环境应激中发挥着重要的作用。然而，人们对弓形虫的线粒体PPMs知之甚少。在本研究中，我们鉴定出三种PPMs，即

PPM8（TGGT1_218590）、

PPM10（TGGT1_254410）和

PPM19（TGGT1_275840），均定位于弓形虫速殖子的线粒体基质中。这三个PPMs中任一PPM编码基因的缺失（Δ

ppm8

、Δ

ppm10

或Δ

ppm19

）均会导致弓形虫速殖子生长抑制与增殖能力减弱，以及另外两个PPMs编码基因信使RNA（mRNA）水平的升高，但不影响弓形虫速殖子在小鼠体内的毒力。Δ

ppm8

ppm10

或Δ

ppm8

ppm19

双重缺失可显著导致弓形虫速殖子体外生长抑制与增殖减缓。由于潜在的突变致死效应，本研究未获得Δ

ppm10

ppm19

和Δ

ppm8

ppm10

ppm19

缺失虫株。上述结果表明，弓形虫线粒体PPMs间存在功能冗余，是弓形虫线粒体生物学研究的新发现，为具有重要兽医和医学意义的弓形虫的防控靶点的发掘提供了新思路。

关键词

Keywords

references

Alday PH , Doggett JS , 2017 . Drugs in development for toxoplasmosis: advances, challenges, and current status . Drug Des Devel Ther , 11 : 273 - 293 . https://doi.org/10.2147/dddt.S60973 https://doi.org/10.2147/dddt.S60973

Dunn D , Wallon M , Peyron F , et al. , 1999 . Mother-to-child transmission of toxoplasmosis: risk estimates for clinical counselling . Lancet , 353 ( 9167 ): 1829 - 1833 . https://doi.org/10.1016/s0140-6736(98)08220-8 https://doi.org/10.1016/s0140-6736(98)08220-8

Guo X , Niemi NM , Hutchins PD , et al. , 2017 . Ptc7p dephosphorylates select mitochondrial proteins to enhance meta bolic function. Cell Rep , 18 ( 2 ): 307 - 313 . https://doi.org/10.1016/j.celrep.2016.12.049 https://doi.org/10.1016/j.celrep.2016.12.049

Macrae JI , Sheiner L , Nahid A , et al. , 2012 . Mitochondrial metabolism of glucose and glutamine is required for intracellular growth of Toxoplasma gondii . Cell Host Microbe , 12 ( 5 ): 682 - 692 . https://doi.org/10.1016/j.chom.2012.09.013 https://doi.org/10.1016/j.chom.2012.09.013

Melo EJ , Attias M , de Souza W , 2000 . The single mitochondrion of tachyzoites of Toxoplasma gondii . J Struct Biol , 130 ( 1 ): 27 - 33 . https://doi.org/10.1006/jsbi.2000.4228 https://doi.org/10.1006/jsbi.2000.4228

Montoya JG , Liesenfeld O , 2004 . Toxoplasmosis . Lancet , 363 ( 9425 ): 1965 - 1976 . https://doi.org/10.1016/s0140-6736(04)16412-x https://doi.org/10.1016/s0140-6736(04)16412-x

Niemi NM , Wilson GM , Overmyer KA , et al. , 2019 . Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis . Nat Commun , 10 : 3197 . https://doi.org/10.1038/s41467-019-11047-6 https://doi.org/10.1038/s41467-019-11047-6

Shi Y , 2009 . Serine/threonine phosphatases: mechanism through structure . Cell , 139 ( 3 ): 468 - 484 . https://doi.org/10.1016/j.cell.2009.10.006 https://doi.org/10.1016/j.cell.2009.10.006

Sidik SM , Huet D , Ganesan SM , et al. , 2016 . A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes . Cell , 166 ( 6 ): 1423 - 1435.e12 . https://doi.org/10.1016/j.cell.2016.08.019 https://doi.org/10.1016/j.cell.2016.08.019

Vercesi AE , Rodrigues CO , Uyemura SA , et al. , 1998 . Respir ation and oxidative phosphorylation in the apicomplexan parasite Toxoplasma gondii. J Biol Chem , 273 ( 47 ):‍ 31040 - 31047 . https://doi.org/10.1074/jbc.273.47.31040 https://doi.org/10.1074/jbc.273.47.31040

Wang ZD , Liu HH , Ma ZX , et al. , 2017 . Toxoplasma gondii infection in immunocompromised patients: a systematic review and meta-analysis . Front Microbiol , 8 : 389 . https://doi.org/10.3389/fmicb.2017.00389 https://doi.org/10.3389/fmicb.2017.00389

Yang C , Arrizabalaga G , 2017 . The serine/threonine phosphatases of apicomplexan parasites . Mol Microbiol , 106 ( 1 ): 1 - 21 . https://doi.org/10.1111/mmi.13715 https://doi.org/10.1111/mmi.13715

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Functional redundancy of three mitochondrial Mg2+/Mn2+-dependent protein phosphatases (PPMs) in Toxoplasma gondii

Functional redundancy of three mitochondrial Mg2+/Mn2+-dependent protein phosphatases (PPMs) in Toxoplasma gondii

DOI：10.1631/jzus.B2400308

摘要

Abstract

关键词

Keywords

references

Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii

Functional redundancy of three mitochondrial Mg²⁺/Mn²⁺-dependent protein phosphatases (PPMs) in Toxoplasma gondii