Drug-assisted synthesis of copper nanoparticles and their induction of cuproptosis and necrosis for breast cancer therapy

Yuewen WANG; Jingjie GAO; Yuying CHENG; Hanling PAN; Hanxiao TANG; Chenguang LIU

doi:10.1631/jzus.B2400163

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Drug-assisted synthesis of copper nanoparticles and their induction of cuproptosis and necrosis for breast cancer therapy

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Correspondence | Updated：2025-12-31

Drug-assisted synthesis of copper nanoparticles and their induction of cuproptosis and necrosis for breast cancer therapy
Drug-assisted synthesis of copper nanoparticles and their induction of cuproptosis and necrosis for breast cancer therapy
药物辅助制备的铜纳米颗粒及其通过诱导细胞铜死亡和坏死的乳腺癌治疗的研究
Journal of Zhejiang University-SCIENCE B Vol. 26, Issue 12, Pages: 1260-1268(2025)
Affiliations：

1.Zhejiang Provincial Engineering Research Center of New Technologies and Applications for Targeted Therapy of Major Diseases, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
2.Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China
Funds：
DOI：10.1631/jzus.B2400163
CLC：
CSTR：
Received：25 March 2024，

Revised：2024-12-26，

Published：31 December 2025
Accepted：

王跃文,高晶杰,程宇莹等.药物辅助制备的铜纳米颗粒及其通过诱导细胞铜死亡和坏死的乳腺癌治疗的研究[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(12):1260-1268.

Yuewen WANG, Jingjie GAO, Yuying CHENG, et al. Drug-assisted synthesis of copper nanoparticles and their induction of cuproptosis and necrosis for breast cancer therapy[J]. Journal of Zhejiang University-SCIENCE B, 2025, 26(12): 1260-1268.
王跃文,高晶杰,程宇莹等.药物辅助制备的铜纳米颗粒及其通过诱导细胞铜死亡和坏死的乳腺癌治疗的研究[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(12):1260-1268. DOI： 10.1631/jzus.B2400163.

Yuewen WANG, Jingjie GAO, Yuying CHENG, et al. Drug-assisted synthesis of copper nanoparticles and their induction of cuproptosis and necrosis for breast cancer therapy[J]. Journal of Zhejiang University-SCIENCE B, 2025, 26(12): 1260-1268. DOI： 10.1631/jzus.B2400163.

摘要

铜离子在铜死亡中发挥着关键作用，过量的Cu（I）离子不仅可与脂酰化二氢脂酰胺

-乙酰转移酶（DLAT）结合，诱导其寡聚，还能降低铁-硫簇蛋白的稳定性，从而共同引发细胞铜死亡。在高氧化水平的肿瘤微环境中，单质铜纳米颗粒能够被氧化生成Cu（I）离子。因此，本研究以药物阿瑞匹坦作为稳定剂，辅助合成无载体的药物-铜纳米粒子。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X射线衍射谱（XRD）、X射线光电子能谱（XPS）和傅立叶变换红外光谱（FIIR）等表征手段，证实铜-阿瑞匹坦纳米颗粒的顺利制备。激光共聚焦显微镜成像显示，该纳米颗粒通过内吞作用进入细胞溶酶体。在溶酶体酸性环境中，铜-阿瑞匹坦纳米颗粒发生pH响应性降解，释放出药物。此外，通过阿瑞匹坦和铜死亡的双模作用，乳腺癌细胞增殖被显著抑制。综上，本研究提供了一种无载体药物-铜纳米颗粒的制备方法，为实现药物介导和铜死亡介导的协同肿瘤治疗提供了新策略。

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