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Promising protective treatment potential of endophytic bacterium
Rhizobium aegyptiacum for ulcerative colitis in ratsPromising protective treatment potential of endophytic bacterium
Rhizobium aegyptiacum for ulcerative colitis in rats- 内生菌
Rhizobium aegyptiacum 对大鼠溃疡性结肠炎的潜在保护性治疗效果研究 - Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Vol. 26, Issue 3, Pages: 286-301(2025)
- Affiliations：
1.Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt
2.Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt
3.Proteomics and Metabolomics Research Program, Department of Basic Research, Children’s Cancer Hospital 57357, Cairo 11441, Egypt
4.Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
5.Department of Pharmaceutics, College of Pharmacy, Prince Sattam bin Abdulaziz University, P.O. Box 173, Alkharj 11942, Saudi Arabia
6.Human Anatomy and Embryology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt
7.Division of Microbiology, Immunology and Biotechnology, Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, University of Tabuk, Tabuk 47713, Saudi Arabia
- Funds：
DOI：10.1631/jzus.B2300777
CLC：- CSTR：
Received：27 October 2023，
Revised：20 February 2024，
Published Online：25 January 2025，
Published：13 March 2025
- Accepted：
Engy ELEKHNAWY,Duaa ELIWA,Sebaey MAHGOUB等.内生菌,Rhizobium aegyptiacum对大鼠溃疡性结肠炎的潜在保护性治疗效果研究[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(03):286-301.
Engy ELEKHNAWY, Duaa ELIWA, Sebaey MAHGOUB, et al. Promising protective treatment potential of endophytic bacterium
Rhizobium aegyptiacum for ulcerative colitis in rats[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(3): 286-301.Engy ELEKHNAWY,Duaa ELIWA,Sebaey MAHGOUB等.内生菌,Rhizobium aegyptiacum对大鼠溃疡性结肠炎的潜在保护性治疗效果研究[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2025,26(03):286-301. DOI： 10.1631/jzus.B2300777.
Engy ELEKHNAWY, Duaa ELIWA, Sebaey MAHGOUB, et al. Promising protective treatment potential of endophytic bacterium
Rhizobium aegyptiacum for ulcerative colitis in rats[J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2025, 26(3): 286-301. DOI： 10.1631/jzus.B2300777.
摘要
溃疡性结肠炎（UC）是由氧化应激和炎性介质增加所导致的一种非特异性的肠道炎症性疾病。本研究首次通过液相色谱-质谱联用技术制备了植物内生细菌
Rhizobium aegyptiacum
提取物，并通过大鼠模型验证其在体内对UC的保护潜力。实验共分为四组，分别为正常对照组（组I）、
R. aegyptiacum
组（组II）、经醋酸（AA）诱导的UC组（组III）和经
R. aegyptiacum
治疗的AA诱导UC组（组IV）。其中，组IV大鼠在UC诱导前一周和后两周每天给予
R. aegyptiacum
处理（0.2 mg/kg）。在实验的最后一天，我们收集各组大鼠结肠组织进行组织学、免疫组化和生化学检测。苏木精-伊红染色、马松染色和PAS染色结果显示，组IV大鼠的结肠出现明显的组织学改善。与对照组相似，其粘膜表面正常，覆盖着平直、完整且薄的刷状边缘；杯状细胞呈品红色，粘膜和黏膜下结缔组织中的胶原纤维分布显著减少。环氧合酶-2（COX-2）免疫染色显示大多数细胞出现弱免疫反应。结肠组织的扫描电镜检查结果与上述组织学评估一致，不同大小的隐窝孔和大量的杯状细胞显示出规律的多边形单元模式。不同组中大鼠结肠组织过氧化氢酶（CAT）、髓过氧化物酶（MPO）、一氧化氮（NO）、白介素-6（IL-6）和白介素-1β（IL-1β）水平的测定结果显示，与组III相比，组IV的CAT水平显著上升（
P
<
0.05），但NO、MPO和炎症细胞因子（IL-6和IL-1β）水平显著降低（
P
<
0.05）。逆转录定量聚合酶链反应（RT-qPCR）结果显示，经AA诱导的UC组（组III）中
TNF-α
基因表达上调，而在经
R. aegyptiacum
治疗的AA诱导UC组（组IV）中则呈显著下调（
P
<
0.05）；与此相反，组IV中的
HO-1
基因则显著上调。本研究实验结果表明，口服
R. aegyptiacum
能恢复和重建粘膜完整，进而改善AA诱导的大鼠UC，并具有抗氧化和抗炎作用。综上，
R. aegyptiacum
在人类UC治疗中可能具有潜在价值，但仍需进一步验证。
Abstract
Ulcerative colitis (UC) is an inflammatory condition of the intestine
resulting from an increase in oxidative stress and pro-inflammatory mediators. In this study
the extract of endophytic bacterium
Rhizobium aegyptiacum
was prepared for the first time using liquid chromatography-mass spectrometry (LC-MS). In addition
also for the first time
the protective potential of
R. aegyptiacum
was revealed using an in vivo rat model of UC. The animals were grouped into four categories: normal control (group I)
R. aegyptiacum
(group II)
acetic acid (AA)-induced UC (group III)
and
R. aegyptiacum
-treated AA-induced UC (group IV). In group IV
R. aegyptiacum
was administered at 0.2 mg/kg daily for one week before and two weeks after the induction of UC. After sacrificing the rats on the last day of the experiment
colon tissues were collected and subjected to histological
immunohistochemical
and biochemical investigations. There was a remarkable improvement in the histological findings of the colon tissues in group IV
as r
evealed by hematoxylin and eosin (H
&
E) staining
Masson’s trichrome staining
and periodic acid-Schiff (PAS) staining. Normal mucosal surfaces covered with a straight
intact
and thin brush border were revealed. Goblet cells appeared magenta in color
and there was a significant decrease in the distribution of collagen fibers in the mucosa and submucosal connective tissues. All these findings were comparable to the respective characteristics of the control group. Regarding cyclooxygenase-2 (COX-2) immunostaining
a weak immune reaction was shown in most cells. Moreover
the colon tissues were examined using a scanning electron microscope
which confirmed the results of histological assessment. A regular polygonal unit pattern was seen with crypt orifices of different sizes and numerous goblet cells. Furthermore
the levels of catalase (CAT)
myeloperoxidase (MPO)
nitric oxide (NO)
interleukin-6 (IL-6)
and interlukin-1β (IL-1β) were determined in the colonic tissues of the different groups using colorimetric assay and enzyme-linked immunosorbent assay (ELISA). In comparison with group III
group IV exhibited a significant rise (
P
<
0.05) in the CAT level but a substantial decline (
P
<
0.05) in the NO
MPO
and inflammatory cytokine (IL-6 and IL-1β) levels. Based on reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
the tumor necrosis factor-α (
TNF-α
) gene expression was upregulated in group III
which was significantly downregulated (
P
<
0.05) by treatment with
R. aegyptiacum
in group IV. On the contrary
the heme oxygenase-1 (
HO-1
) gene was substantially upregulated in group IV. Our findings imply that the oral consumption of
R. aegyptiacum
ameliorates AA-induced UC in rats by restoring and reestablishing the mucosal integrity
in addition to its anti-oxidant and anti-inflammatory effects. Accordingly
R. aegyptiacum
is potentially effective and
beneficial in human UC therapy
which needs to be further investigated in future work.
关键词
Keywords
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